Unlock instant, AI-driven research and patent intelligence for your innovation.
Detection kit for budesonide metabolism marker and detection method and application thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology for detection kits and metabolic markers, used in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of cumbersome operation and time-consuming, and simplify the sequencing process and time Effect
Pending Publication Date: 2021-11-19
菲思特(上海)生物科技有限公司
View PDF6 Cites 2 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0005] At present, the methods of obtaining blood DNA mainly include traditional column extraction and magnetic bead extraction, both of which are time-consuming and cumbersome to operate
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0042] Embodiment 1, preparation and use of kit
[0043] (1) Specific primer design
[0044] The kit of the present invention designs specific amplification primers and sequencing primers for the polymorphism of the GLCCI1 (A-1106G) gene, and is used for pyrophosphate PCR detection. The gene polymorphism sequence is subject to the public sequence in Genebank, and the primer sequences are shown in Table 1 below.
[0045] Table 1
[0046]
[0047] (2) Kit composition
[0048] The detection kit includes the components shown in Table 2 below:
[0062] The instruments adopted in the present invention are as follows: thermostat, pyrosequencer (Wuhan First Biotechnology Co., Ltd.).
[0063] 1) Take 30 μL EDTA anticoagulated whole blood sample in a PCR amplification tube;
[0064] 2) Add 100 μL of sample treatment solution and 4ul of magnetic beads, and let stand for 5 minutes;
[0065] 3) Place the PCR amplification tube on the magnetic stand, and suck out the mixed solution from the opposite side of the magnetic beads after all the magnetic beads are adsorbed to one side;
[0066] 4) Add amplification reagent 1 into the dry powder of amplification reagent 2, fully dissolve and mix;
[0067] 5) Add 25ul of the prepared PCR reaction solution to the PCR amplification tube obtained in step 4), fully mix the magnetic beads with the PCR reaction solution, centrifuge, and perform a constant temperature reaction;
[0068] 6) Amplify using a PCR instrument, the reaction system is 25...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention discloses a detection kit for a budesonidemetabolism marker and a detection method and application thereof. The kit designs a specific amplification primer and a sequencing primer aiming at the polymorphism of GLCCI1A-1106G, and the kit comprises the following components: a sample treating fluid, magnetic beads, an amplification reagent 1, an amplification reagent 2, a GLCCI1A-1106G sequencing primer and a positive control. The detection kit is mainly optimized from two aspects: on one hand, a mode of extracting and amplifying in the same tube is adopted, so that the risk of nucleic acid loss due to repeated tube transfer during extraction is avoided; on the other hand, rapid isothermal amplification is carried out by adding an anti-inhibitor; the invention aims to detect the gene polymorphism of the curative effect of budesonide by combining rapid DNA (deoxyribonucleic acid) preparation, constant-temperature PCR (polymerasechain reaction) amplification and pyrosequencing technologies, and provides suggestions from the gene perspective for clinical personalized medication.
Description
technical field [0001] The invention relates to a detection kit for budesonidemetabolic markers and a detection method and application thereof, belonging to the field of gene detection. Background technique [0002] Budesonide is a glucocorticoid with highly effective local anti-inflammatory effects, which can enhance the stability of endothelial cells, smooth muscle cells and lysosomal membranes, inhibit immune reactions and reduce antibody synthesis, thereby preventing the production of allergic active media such as histamine. The release and activity are reduced, and it can alleviate the enzymatic process stimulated by the combination of antigen and antibody, inhibit the synthesis and release of bronchoconstriction substances, and reduce the contraction response of smooth muscle. It is clinically used in patients with glucocorticoid-dependent or non-dependent bronchial asthma and asthmatic chronic bronchitis. Refractoryasthma refers to asthma that cannot be well contro...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More 
