A next-generation sequencing-based method for STR typing of the locus D8S1179

Abstract

The invention relates to the field of molecular biology and particularly discloses a specific primer pair for amplifying a gene D8S1179. The specific primer pair comprises D8S1179-FP:5'-TTTGTATTTCATGTGTACATTCGTAATTC-3' and D8S1179-RP:5'-ACCTATCCTGTAGATTATTTTCACTGATTG-3'. The invention further provides an STR typing method for a loca D8S1179 based on next generation sequencing and particularly provides a primer combination for being connected with a proper adaptor. The STR typing method is applied to the STR typing of the loca D8S1179. According to the primer pair capable of amplifying the gene D8S1179, after concentrations of different sample products subjected to PCR amplification, the equivalent mixing is carried out, and a genome library is constructed according to a BIOO RAPID DNA kit; the library is input into a computer by virtue of a next generation sequencer, data is subjected to bioinformatics analysis, and the typing results such as times of repetition of NDA molecules with same length, SNP of the loca and the difference among side wings of the loca of D8S1179 can be found.

Description

technical field [0001] The present invention relates to the field of molecular biology, in particular to a method for typing STRs based on the gene locus D8S1179 of next-generation sequencing. Background technique [0002] One of the basic tasks of forensic physical evidence is to solve the problem of individual identification. Forensic DNA analysis plays a vital role in clarifying the nature of the case and identifying the suspects, parties and victims. Forensic DNA analysis is a science and technology that uses modern DNA technology to analyze the distribution and transmission of DNA genetic markers in the population, to determine the consistency and genetic relationship of the analyzed samples, and to provide evidence for the investigation and judicial trial. Since 1985, Jeffereys and others established the first generation of typing technology - DNA fingerprinting technology, together with the later development of PCR amplified fragment length polymorphism analysis techn...

AI Technical Summary

Problems solved by technology

[0003] However, with the continuous improvement of DNA extraction ability and detection technology sensitivity, forensic physical evidence detection has developed from constant detection to trace or even trace detection. In the practice of physical evidence detection, the inherent shadow peaks of STR typing technology, Heterozygous locus amplification imbalance (heterozygote peak imbalance), allele loss (allelic drop-out), and low copy number template low copy number) make the genetic information obtained by traditional STR typing technology limited. There are application limitations in physical evidence inspection, and it is difficult to satisfactorily solve trace or complex problems in individual identification

Images