Analysis method for multiplex amplification STR data

An analysis method and composite amplification technology, which are applied in the field of STR data analysis, can solve the problems of lack of specific processing of multiplex PCR sequencing data, inability of analysis data to match capillary electrophoresis data, and low accuracy.

Pending Publication Date: 2021-05-11
深圳百人科技有限公司
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Problems solved by technology

[0005] 1. Poor adaptability: the existing sequence analysis technology only targets whole-genome off-machine data, and lacks specific processing for multiple PCR sequencing data
[0006] 2. Accuracy: The STR database is based on the typing standard of the CODIS system, and the existing analysis data cannot m

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  • Analysis method for multiplex amplification STR data
  • Analysis method for multiplex amplification STR data
  • Analysis method for multiplex amplification STR data

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Embodiment Construction

[0036] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0037] The STR locus is an important genetic marker in human genetics, and there is one every 6000-10000 bases in the human genome on average. Because of its rich polymorphism, large number, easy amplification and convenient detection, it is widely used in individual identification and kinship identification. According to reports, more than 8,000 STR loci have been found in the human genome, and all of them have genetic polymorphisms. Therefore, the detection...

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Abstract

The invention discloses an analysis method for multiplex amplification STR data. The analysis method comprises the following steps: 1, site reference information: inputting data; 2, quality control (preprocessing): filtering data; 3, an STR typing method: a) searching for optimal matching, and b) referencing internal reference data. According to the method, high typing accuracy and timeliness can be guaranteed, CODIS system automatic conversion of STR loci can be achieved, the consistency of the STR loci and capillary electrophoresis data reaches up to 100% in the aspect of accuracy, and the analysis time is obviously shorter than that of mainstream analysis software in the market in the aspect of timeliness. Therefore, as an important supplementary method for whole genome sequence analysis, the process has great practical significance to forensic genetics.

Description

technical field [0001] The invention relates to the technical field of analysis of STR data, in particular to an analysis method for multiple amplification STR data. Background technique [0002] In recent years, deoxyribonucleic acid (DNA) sequencing technology has been developed by leaps and bounds, and the continuous development of next generation sequencing technology (Next generation sequencing) has made whole genome sequencing possible. However, due to the limitations of high cost and large data consumption, whole genome sequencing is not suitable for the analysis of complex repetitive DNA regions, including what we call short tandem repeats (STR). Therefore, taking multiplex PCR and probe capture as examples, many target sequence acquisition methods matching next-generation sequencing are also constantly maturing and developing. Compared with probe capture, multiplex polymerase chain reaction (PCR) has a better acquisition efficiency for STR sites. Therefore, the mu...

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Application Information

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IPC IPC(8): G16B30/00
CPCG16B30/00
Inventor 苗鑫垚于慧云李博文沈悦生赵梓丞李梦瑶贺小兰
Owner 深圳百人科技有限公司
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