Method for enhancement of naturally occurring cytoplasmic male sterility and for restoration of male fertility and uses thereof in hybrid crop prodn.

A male sterility and cytoplasmic technology, applied in biochemical equipment and methods, cells modified by introducing foreign genetic material, applications, etc., can solve the problem of reducing the total yield of "hybrid" mixtures, leakage, CMS seed contamination, etc. question

Inactive Publication Date: 2000-08-30
MCGILL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cost-effectiveness ratio of producing hybrids using this system is limited by the fact that pol-induced male sterility has the potential to be incomplete or "leaky", especially when female lines are exposed to warmer growing conditions Time
As a result, pol hybrid seeds may be contaminated with CMS seeds produced by self-pollination of female lines
Since these CMS plants are male sterile and unable to set seeds spontaneously, their presence can significantly reduce the overall yield of "hybrid" mixtures

Method used

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  • Method for enhancement of naturally occurring cytoplasmic male sterility and for restoration of male fertility and uses thereof in hybrid crop prodn.
  • Method for enhancement of naturally occurring cytoplasmic male sterility and for restoration of male fertility and uses thereof in hybrid crop prodn.
  • Method for enhancement of naturally occurring cytoplasmic male sterility and for restoration of male fertility and uses thereof in hybrid crop prodn.

Examples

Experimental program
Comparison scheme
Effect test

Embodiment I

[0098] Use of the AP3 / A9-A6e construct as a dominant fertility restorer gene in Manqing

[0099] In this example, through gene transformation will Figure 4 The A9 / A6e construct shown is introduced into the genome of a species such as Westar, usually by (pol) cytoplasmic sterile. The gene was introduced into the pol cytoplasmic line and the resulting transgene obtained was expected to be partially male fertile. This line was self-pollinated to produce a partially male fertile restorer line homozygous for the A9 / A6e transgene. This line can then be crossed with a different pol CMS line to produce a restored fertile F1 hybrid.

Embodiment II

[0101] Use of AP3 / C4-ORF constructs to enhance pol cytoplasmic male sterility in Manqing

[0102] Induction of sterility by pol cytoplasm was incomplete for most of the Cranberry genotypes, especially at higher temperatures. In hybrid seed production operations, the production of residual pollen results in variable levels of selfing of the CMS lines. Plants grown from these seeds are not hybrids and will have a reduced ability to self-seed. Together, these factors can significantly reduce the yield of "hybrid" seed batches. In this example, using Figure 5 The indicated C4-ORF dominantly transmits the sterile property to reduce or eliminate residual pollen production in the CMS lines, thereby increasing the percentage of hybrid seed obtained and thus increasing the yield of hybrid seed batches. Enhanced Genetic Transformation The C4-ORF construct was introduced into a fully dominant fertile Westar (nap) line to generate a partially dominant fertile line containing the C4-OR...

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Abstract

The present invention relates to methods for enhancement of naturally occurring cytoplasmic male sterility and for restoration of male fertility and uses thereof in hybrid crop production. There is also disclosed a method for restoration of male fertility to cytoplasmic male sterile plants; which comprises the steps of: a) introducing into the nucleus of a plant cell a gene construct essentially consisting of a sequence encoding a mitochondrial transit peptide fused upstream of and in frame with an edited form of a normal mitochondrial gene that is co-transcribed with an unusual CMS-associated mitochondrial gene; b) selecting for plan cells that have acquired the gene construct in step a); and c) inducing regeneration of selected plant cells to produce a mature plant.

Description

Background of the invention [0001] (a) Field of Invention [0002] The present invention relates to methods of increasing naturally occurring cytoplasmic male sterility and methods of restoring male fertility and their use in the production of hybrid crops. [0003] (b) Description of prior art [0004] For many plants, hybrids formed by crossing two or more different plants provide higher yields than the parent plants themselves. For example, in Canada's most important crop, canda (Brassica napus, campestris), artificially produced hybrids have yields up to 50% higher than their parent lines. This phenomenon, called heterosis, has been most successfully applied in maize (Zea mays). Hybrid corn accounts for about 90 percent of North American crops. [0005] In order to produce hybrid seeds on a commercial scale, self-pollination of the hybrid seed parents must be prevented. This is a relatively simple matter in maize because the male or pollen-producing organs (stamens) a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/00C07K14/415C12N5/10C12N15/09C12N15/29C12N15/82
CPCC12N15/8289C07K14/415
Inventor 格雷戈里·G·布朗
Owner MCGILL UNIV
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