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Fermentation process for preparing intestinal membrane protein

A fermentation process and intestinal membrane protein technology, which is applied in the field of intestinal membrane protein extraction technology, can solve the problems of ineffective use of resources, high cost, low efficiency, etc., and achieve high raw material utilization rate, improved production efficiency, and perfect process.

Inactive Publication Date: 2017-09-22
NANTONG RENSHOU FOOD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In the past, the intestinal membrane protein production process was not perfect enough to effectively utilize resources, resulting in insufficient utilization of raw materials, and a large amount of waste water was generated, resulting in higher costs and low efficiency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] A fermentation process for the preparation of intestinal membrane protein, which is specifically in the following steps:

[0013] (1) Enzymatic hydrolysis: firstly add Bacillus licheniformis protease to the waste casing liquid, the amount of enzyme added is 2g per 10L waste liquid casing, then adjust the pH of the mixture to 9 with alkaline solution, raise the temperature to 60°C, and keep the temperature for 2 hours , and then add water citrullin, the amount of enzyme added is 2g per 10L of waste casing liquid, kept for 2 hours, and finally heated to 70 °C, kept for 2 minutes, and filtered through a 100-mesh stainless steel filter to obtain the enzymatic hydrolysis solution; (2) Adsorption and filtration: Cool the enzymatic hydrolysis solution obtained in step (2) to 50°C, add resin, stir and adsorb for 10 hours, filter out the resin with 80-mesh fiber cloth filter bag, and collect the filtrate; (3) Washing and elution: ①Put the fiber bag containing the resin into clea...

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PUM

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Abstract

The invention relates to an intestinal membrane protein extracting process, in particular to a fermentation process for preparing intestinal membrane protein. Waste casing liquid is used as a raw material, and resin and filtrate are respectively obtained through enzymolysis, resin adsorption and filtration; the resin is subjected to washing, elution, precipitation and drying to obtain heparin sodium salt; the filtrate is concentrated and dried to obtain intestinal membrane protein. The fermentation process has the advantages that the process is perfect, the cost is low, the raw material utilization rate is high, no waste is produced, energy is saved, emission is reduced, and the production efficiency is greatly improved.

Description

technical field [0001] The invention relates to an extraction process of intestinal membrane protein, in particular to a fermentation process for preparation of intestinal membrane protein. Background technique [0002] In the past, the production process of intestinal membrane protein was not perfect enough to effectively utilize resources, resulting in insufficient utilization of raw materials, and also produced a large amount of waste water, increased cost and low efficiency. SUMMARY OF THE INVENTION [0003] In order to solve the above problems, the present invention provides a fermentation process for the preparation of intestinal membrane protein, which is mature in technology, low in cost and high in efficiency. [0004] In order to achieve the above object, the present invention adopts the following technical solutions: a fermentation process for the preparation of intestinal membrane protein, which is specifically in the following steps: [0005] (1) Enzy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/30C07K1/22
CPCC12P21/06C07K1/22C07K1/30C07K1/34C07K1/36
Inventor 冯爱军刘春捷
Owner NANTONG RENSHOU FOOD CO LTD
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