Process using intestinal casing waste liquid as raw material for production of sodium heparin and intestinal membrane protein
A technology of intestinal membrane protein and heparin sodium, which is applied in the field of extraction technology of heparin sodium and intestinal membrane protein, can solve problems such as waste, and achieve the effects of improving environment, simple process and improving economic benefits.
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[0020] Below we will further illustrate the present invention in conjunction with specific examples.
[0021] The process of producing heparin sodium and intestinal membrane protein from waste liquid of casing comprises the following steps:
[0022] (1) Enzymatic hydrolysis: first add 2709 alkaline protease to the casing waste liquid, the amount of enzyme added is 1g of enzyme per 1L of casing waste liquid, then adjust the pH of the mixture to 8.8 with lye, raise the temperature to 55°C, and keep it warm for 3 hours , then add papain, the amount of enzyme added is 1g of enzyme per 1L of casing waste liquid, keep warm for 1 hour, and finally raise the temperature to 88°C, keep warm for 18 minutes, and filter with 100 mesh filter cloth to obtain the enzymatic solution;
[0023] (2) Adsorption and filtration: Cool the enzymolysis solution obtained in step (2) to 56°C, add ZGA398 resin, stir and adsorb for 8 hours, filter out the ZGA398 resin with an 80-mesh nylon cloth filter bag...
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