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Process using intestinal casing waste liquid as raw material for production of sodium heparin and intestinal membrane protein

A technology of intestinal membrane protein and heparin sodium, which is applied in the field of extraction technology of heparin sodium and intestinal membrane protein, can solve problems such as waste, and achieve the effects of improving environment, simple process and improving economic benefits.

Inactive Publication Date: 2014-10-29
GUANGYUAN HAITIAN IND
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0002] Casing waste liquid refers to a kind of waste liquid when animal intestines are scraped to remove mucous membranes (including attachments or mucus attached to mucous membranes) in the industrial process of preparing casings. The quality of mucous membranes and water in the waste liquid is The ratio is about 1:5. Many casing processing enterprises treat the casing waste liquid as wastewater and directly enter the subsequent sewage treatment process, resulting in unnecessary waste.

Method used

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Embodiment Construction

[0020] Below we will further illustrate the present invention in conjunction with specific examples.

[0021] The process of producing heparin sodium and intestinal membrane protein from waste liquid of casing comprises the following steps:

[0022] (1) Enzymatic hydrolysis: first add 2709 alkaline protease to the casing waste liquid, the amount of enzyme added is 1g of enzyme per 1L of casing waste liquid, then adjust the pH of the mixture to 8.8 with lye, raise the temperature to 55°C, and keep it warm for 3 hours , then add papain, the amount of enzyme added is 1g of enzyme per 1L of casing waste liquid, keep warm for 1 hour, and finally raise the temperature to 88°C, keep warm for 18 minutes, and filter with 100 mesh filter cloth to obtain the enzymatic solution;

[0023] (2) Adsorption and filtration: Cool the enzymolysis solution obtained in step (2) to 56°C, add ZGA398 resin, stir and adsorb for 8 hours, filter out the ZGA398 resin with an 80-mesh nylon cloth filter bag...

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Abstract

The invention discloses a process using intestinal casing waste liquid as a raw material for production of sodium heparin and intestinal membrane protein, and the process is characterized in that, the preparation process comprises the following steps: the intestinal casing waste liquid is taken as the raw material for enzymolysis, resin adsorption and filtration to respectively obtain resin and filtrate; the resin is washed, eluted, precipitated and dried to obtain the sodium heparin, and the filtrate is concentrated and dried to obtain the intestinal membrane protein. The process comprehensively uses the intestinal casing waste liquid, is simple, not only greatly improves the economic benefit, but also helps to improve the environment.

Description

technical field [0001] The invention relates to a process for extracting heparin sodium and intestinal membrane protein, in particular to a process for producing heparin sodium and intestinal membrane protein by using waste liquid of casing as raw material. Background technique [0002] Casing waste liquid refers to a kind of waste liquid when animal intestines are scraped to remove mucous membranes (including attachments or mucus attached to mucous membranes) in the industrial process of preparing casings. The quality of mucous membranes and water in the waste liquid is The ratio is about 1:5. Many casing processing enterprises treat the casing waste liquid as wastewater and directly enter the subsequent sewage treatment process, resulting in unnecessary waste. Contents of the invention [0003] In view of the above-mentioned shortcomings, the purpose of the present invention is to provide a process for producing heparin sodium and intestinal membrane protein using waste ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/10C12P21/06
Inventor 李海生
Owner GUANGYUAN HAITIAN IND
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