Low serum efficiency mycoplasma gallisepticam attenuated strain culture medium and preparation method thereof

A technology of Mycoplasma gallisepticum and culture medium, applied in the field of veterinary biology, can solve the problems of affecting the immune efficacy of vaccines, increasing allergic stress reactions, etc., and achieve the effect of reducing allergic stress reactions and increasing the titer of live bacteria

Active Publication Date: 2013-05-01
兆丰华生物科技(南京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The amount of serum required for the above-mentioned traditional cultivation of Mycoplasma gallisepticum culture medium is 5%-25% higher than the serum amount of the present invention, an

Method used

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  • Low serum efficiency mycoplasma gallisepticam attenuated strain culture medium and preparation method thereof
  • Low serum efficiency mycoplasma gallisepticam attenuated strain culture medium and preparation method thereof
  • Low serum efficiency mycoplasma gallisepticam attenuated strain culture medium and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The preparation of culture medium of the present invention

[0025] Basal medium:

[0026]

[0027] Sterilize at 116°C for 30 minutes. After cooling, add the following auxiliary medium components under aseptic conditions to make a culture medium for Mycoplasma gallisepticum.

[0028] Auxiliary medium:

[0029]

[0030]

[0031] Dissolve the components of the basal medium (1)-(8) one by one into 500ml distilled water for high-pressure sterilization, add auxiliary medium after cooling, and adjust the pH to 7.7 with 1mol / L sodium hydroxide solution to obtain low-serum and high-efficiency Mycoplasma gallisepticum culture base.

Embodiment 2

[0033] The preparation of culture medium of the present invention

[0034] Basal medium:

[0035]

[0036] Sterilize at 116°C for 30 minutes. After cooling, add the following auxiliary medium components under aseptic conditions to make a medium for cultivating attenuated strains of Mycoplasma gallisepticum.

[0037] Auxiliary medium:

[0038]

[0039] Dissolve the components of the basic medium (1)-(8) one by one in 800ml distilled water and sterilize under high pressure, add auxiliary medium after cooling, and adjust the pH to 7.7 with 1mol / L sodium hydroxide solution to obtain low-serum and high-efficiency Mycoplasma gallisepticum culture base.

Embodiment 3

[0041] Use Mycoplasma gallisepticum (F36) to carry out comparison culture test to Mycoplasma gallisepticum (F36) by Mycoplasma medium of the present invention, improved Frey's medium, Mycoplasma medium respectively:

[0042] 1 Preparation of culture medium of the present invention

[0043] Basal medium:

[0044]

[0045]

[0046] Sterilize at 116°C for 30 minutes. After cooling, add the following auxiliary medium components under aseptic conditions to make a culture medium for Mycoplasma gallisepticum.

[0047] Auxiliary medium:

[0048]

[0049] Dissolve the components of the basic medium (1)-(8) one by one in 1000ml distilled water and sterilize under high pressure, add auxiliary medium after cooling, and adjust the pH to 7.7 with 1mol / L sodium hydroxide solution to obtain low-serum and high-efficiency Mycoplasma gallisepticum culture base.

[0050] 2 Preparation of Modified Frey's Medium

[0051] base fluid

[0052]

[0053] Dissolve in 1000ml deionized wat...

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Abstract

The present invention relates to a low serum efficiency mycoplasma gallisepticam attenuated strain culture medium and a preparation method thereof, and belongs to the technical field of veterinary biology. The culture medium comprises: (1) a base culture medium; and (2) an auxiliary culture medium, wherein the auxiliary culture medium mainly comprises MEM, yeast extract powder, tryptone, glucose, an inorganic salt, and the like, and growth, high titer and stability of the semi-finished product can be ensured with the auxiliary culture medium. According to the present invention, a titer of the semi-finished product bacterial liquid prepared by using the preparation method is up to 10<11> CCU/ml; and the culture medium adopts reduced pig serum to culture mycoplasma gallisepticam so as to reduce allergic stress reactions on chicken by heterologous pig serum, consider animal biosafety, improve antigen titer, and reduce production cost.

Description

technical field [0001] The invention belongs to the technical field of veterinary biology, and relates to a low-serum high-efficiency culture medium for attenuated mycoplasma gallisepticum and a preparation method thereof. Background technique [0002] Mycoplasma gallisepticum mainly causes chronic respiratory diseases in chickens and turkeys. It is a pathogen that seriously endangers poultry production. The development of growing chickens is hindered and the feed remuneration is reduced; due to the secondary or concomitant occurrence of other diseases caused by the immunosuppression of infected chickens, the main loss to the chicken industry is that the death rate of diseased chickens has increased significantly. The combined use of live Mycoplasma gallisepticum vaccine and inactivated vaccine can reduce the horizontal and vertical transmission of Mycoplasma gallisepticum, but the premise of a high-quality vaccine requires a good culture medium to support it. [0003] The ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/35
Inventor 不公告发明人
Owner 兆丰华生物科技(南京)有限公司
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