Low-serum culture medium for efficiently culturing mycoplasma hyopneumoniae and preparation method thereof
A technology of mycoplasma hyopneumoniae and culture medium, which is applied in the field of high-efficiency mycoplasma hyopneumoniae culture medium and its preparation, can solve the problems of increasing allergic stress response of pigs, low titer of viable bacteria, and affecting vaccine immune efficacy, etc., so as to reduce allergic reactions The effect of stimulating the reaction and increasing the titer of live bacteria
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Embodiment 1
[0033] 1, the preparation of culture medium of the present invention
[0034] 1.1A solution:
[0035] (1) MEM 6g
[0036] (2) Yeast extract powder 2g
[0037] (3) Tryptone 6g
[0038] (4) Glucose 6g
[0039] (5) 1ml of phenol red solution with a mass concentration of 1%
[0040] (6) Na 2 HPO 4 12H2O 1g
[0041] (7) KH 2 PO4 0.05g
[0042] (8) NaCl 2g
[0043] Liquid B:
[0044] (9) Fresh yeast powder 120g
[0045] (10) Glutamine 0.2g
[0046] (11) Arginine monohydrochloride 0.15g
[0047] (12) Penicillin 200u / ml
[0048] Healthy Pig Serum 100ml
[0049] 2. The method for preparing a high-efficiency Mycoplasma hyopneumoniae culture medium, comprising the steps of:
[0050] (1) Preparation of liquid A:
[0051] Take the above-mentioned liquid A (1)~(8) and dissolve them in 500ml distilled water one by one, autoclave at 116°C for 30 minutes and set aside;
[0052] (2) Preparation of liquid B:
[0053] Weigh the components (9) to (12) in claim 1 according to the...
Embodiment 2
[0057] 1, the preparation of culture medium of the present invention
[0058] 1.1A solution:
[0059] (2) MEM 7g
[0060] (3) Yeast extract powder 4g
[0061] (4) Tryptone 8g
[0062] (5) Glucose 8g
[0063] (5) 2ml of phenol red solution with a mass concentration of 1%
[0064] (6) Na 2 HPO 4 12H2O 2g
[0065] (7) KH 2 PO 4 0.09g
[0066](8) NaCl 3g
[0067] Liquid B:
[0068] (9) Fresh yeast powder 140g
[0069] (10) Glutamine 0.3g
[0070] (11) Arginine monohydrochloride 0.15g
[0071] (12) Penicillin 400u / ml
[0072] Healthy Pig Serum 150ml
[0073] 2. The method for preparing a high-efficiency Mycoplasma hyopneumoniae culture medium, comprising the steps of:
[0074] (1) Preparation of liquid A:
[0075] Take the above-mentioned liquid A (1)~(8) and dissolve them in 500ml distilled water one by one, autoclave at 116°C for 30 minutes and set aside;
[0076] (3) Preparation of liquid B:
[0077] Weigh the components (9) to (12) in cl...
Embodiment 3
[0081] Using Mycoplasma hyopneumoniae to carry out comparative culture test on Mycoplasma hyopneumoniae through the Mycoplasma culture medium of the present invention, KM2 culture medium, PPLO Mycoplasma culture medium respectively.
[0082] 1, the preparation of culture medium of the present invention
[0083] Liquid A:
[0084] (1) MEM 6.3g
[0085] (2) Yeast extract powder 2.7g
[0086] (3) Tryptone 6.7g
[0087] (4) Glucose 6.7g
[0088] (5) 1.5ml of phenol red solution with a mass concentration of 1%
[0089] (6) Na 2 HPO 4. 12H 2 O 1.2g
[0090] (7) KH 2 PO 4 0.08g
[0091] (8) NaCl 2.3g
[0092] Liquid B:
[0093] (9) Fresh yeast powder 130g
[0094] (10) Glutamine 0.25g
[0095] (11) Arginine monohydrochloride 0.17g
[0096] (12) Penicillin 300u / ml
[0097] Healthy Pig Serum 120ml
[0098] 2. The method for preparing a high-efficiency Mycoplasma hyopneumoniae culture medium, comprising the steps of:
[0099] (1) Prepa...
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