Mycoplasma canis culture medium and application thereof
A technology of Mycoplasma canis and liquid culture medium, applied in the field of microorganisms, can solve the problems of slow growth, difficult separation, and high nutritional requirements of the culture medium, and achieve the effects of rapid separation and analysis, and high titer of viable bacteria
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Embodiment 1
[0016] Example 1 Mycoplasma canis high-efficiency medium and its preparation
[0017] The invention provides two mediums suitable for rapid and effective cultivation of Mycoplasma canis, which are respectively QG2 and QG5 mediums, each of which includes a liquid medium and a solid medium.
[0018] The formula of QG2 liquid medium is: 60%-64% of FM-4 basic culture medium, 10%-12% of yeast extract (30%), 5%-8% of calf serum, 1.0%-2.0% of dog serum, polypeptide T2 (4ng / ml) 1.0%-1.5%, L-asparagine (1g / ml) 0.02%-0.05%, NaCl (5g / ml) 0.12%-0.16%, penicillin (1×10 5 IU / ml) 1.2%, phenol red (1%) 0.003%, pH adjusted to 7.6-7.8. Preferably, the optimal QG2 liquid medium formula is: FM-4 basal culture medium 62%, yeast extract (30%) 10%, calf serum 6%, canine serum 1.5%, polypeptide T2 (4ng / ml) 1.2 %, L-asparagine (1g / ml) 0.04%, NaCl (5g / ml) 0.13%, penicillin (1×10 5 IU / ml) 1.2%, phenol red (1%) 0.003%, pH adjusted to 7.6-7.8.
[0019] The formula of QG5 liquid medium is: FM-4 basic c...
Embodiment 2
[0024] Example 2 Effectiveness of Mycoplasma canis high-efficiency medium
[0025] Then test the effect of QG medium on the cultivation of Mycoplasma canis. In a pet breeding base in Fuzhou City, Fujian Province, anticoagulant venous blood was collected from dogs with suspected mycoplasma disease (polymorphic spheroid microorganisms can be seen on blood smear Giemsa staining), and 0.1ml of each was added to 100ml of QG liquid culture medium (including QG2 and QG5) and three conventional mycoplasma liquid media (including FM-4, PPLO and SP4 medium, as a control) (the first inoculation), placed at 37°C and 6% CO 2 Cultivate under the same conditions until the color of the medium changes from red to yellow, and then take 10ml of the culture solution and add it to a new 90ml of the respective liquid medium. Cultivate under the same conditions until the medium changes color (the second inoculation), and repeat the transfer once (the second inoculation). Three inoculations), and fi...
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