Wild jujube seeds extract and preparation method and use thereof
A kind of jujube seed extract and technology of jujube seed, applied in the direction of drug combination, medical formula, plant raw materials, etc., can solve the problems of drug resistance of bacteria, difficulty in treating and preventing bacterial infectious diseases, etc., and achieve low toxicity, Good curative or preventive effect, effect of ensuring food safety
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Embodiment 1
[0025] The preparation of embodiment 1 Suanzaoren extract
[0026] Grind 100g of jujube kernels and pass through a 10-mesh sieve. Add 6 times the amount (ml / g) of petroleum ether to the screened matter and soak for 6 hours to degrease. Degrease 6 times. After the degreasing is complete, evaporate the petroleum ether to dryness. Then add 75% alcohol of 6 times of amount (ml / g) to the dregs to soak and extract, soak for 4 hours each time, extract 4 times continuously, add 3 times of amount (ml / g) of water after removing the alcohol and stir to mix. Uniform, then add 4 times of amount (ml / g) water-saturated n-butanol therein and extract continuously 4 times, and concentrate, make the Suanzaoren jelly paste of 5g, its extraction rate is 5%.
[0027] Qualitative and quantitative determination of jujuboside A and B in jujube seed extract
[0028] Qualitative experiment:
[0029] Take 2g of the above Jujube Seed Gum Paste, add 25ml of water-saturated n-butanol solution, heat to dis...
Embodiment 2
[0053] Example 2 Preparation of Jujube Seed Extract
[0054] Grind 100g of jujube kernels and pass through a 10-mesh sieve, add 2 times the amount (ml / g) of petroleum ether to the sieved matter, soak and degrease for 3 hours, degrease 8 times, evaporate the petroleum ether to dry after the degreasing is complete. Then add 73% alcohol of 4 times the amount (ml / g) of the dregs to soak and extract, soak for 1 hour each time, and extract 7 times continuously, add 3 times the amount (ml / g) of water after removing the alcohol and stir to mix. homogeneous, then add 2 times the amount (ml / g) of water-saturated n-butanol to extract continuously for 2 times, and concentrate to obtain 4.1g of Suanzaoren jelly paste, with an extraction rate of 4.1%.
[0055] Qualitative and quantitative determination of jujuboside A and B in jujube seed extract
[0056] Qualitative experiment:
[0057] The assay method of qualitative experiment is the same as embodiment 1, and in the obtained test sampl...
Embodiment 3
[0066] Example 3 Preparation of Jujube Seed Extract
[0067] Grind 150g of jujube kernels and pass through a 10-mesh sieve. Add 8 times the amount (ml / g) of petroleum ether to the screened matter and soak for 7 hours to degrease. Degrease 5 times. After the degreasing is complete, evaporate the petroleum ether to dryness. Then add 8 times the amount (ml / g) of 71% alcohol to the dregs to soak and extract, soak for 2 hours each time, and extract 2 times continuously, add 5 times the amount (ml / g) of water after removing the alcohol and stir to mix. homogeneous, then add 6 times of amount (ml / g) of water-saturated n-butanol to extract continuously for 6 times, and concentrate to obtain 9.6g Suanzaoren jelly paste, with an extraction rate of 6.4%.
[0068] Qualitative and quantitative determination of jujuboside A and B in jujube seed extract
[0069] Qualitative experiment:
[0070] The assay method of qualitative experiment is the same as embodiment 1, and in the obtained test...
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