51 results about "Genetic Technique" patented technology

One embodiment of the present invention provides a system that optimizes a regression model which predicts a signal as a function of a set of available signals. During operation, the system receives training data for the set of available signals from a computer system during normal fault-free operation. The system also receives an objective function which can be used to evaluate how well a regression model predicts the signal. Next, the system initializes a pool of candidate regression models which includes at least two candidate regression models, wherein each candidate regression model in the pool includes a subset of the set of available signals. The system then optimizes the regression model by iteratively: (1) selecting two regression models U and V from the pool of candidate regression models, wherein regression models U and V best predict the signal based on the training data and the objective function; (2) using a genetic technique to create an offspring regression model W from U and V by combining parts of the two regression models U and V; and (3) adding W to the pool of candidate regression models.

The present invention relates generally to recombinant genetic technology. More particularly, the present invention relates to compositions and methods for use in selection and isolation of nucleic acid molecules. The invention further relates to methods for the preparation of individual nucleic acid molecules and populations of nucleic acid molecules, as well as nucleic acid molecules produced by these methods. The invention also relates to screening and/or selection methods for identifying and/or isolating nucleic acid molecules which have one or more common features (e.g., characteristics, activities, etc) and populations of nucleic acid molecules which share one or more features.

The invention discloses a core primer composition for Brassica SSR (simple sequence repeats) and belongs to the technical field of molecular inheritance. The core primer composition comprises 190 core SSR primer pairs for 19 chromosomes in Brassica A and C genomes; and 10 SSR primer pairs are uniformly distributed on each chromosome, which have high polymorphism levels, substantially consistent annealing temperature and clear amplification band patterns. The core SSR primer composition is applied to increasing the molecular marking efficiency by 3 to 5 times without increasing the working costs, and is particularly suitable for the identification, the genetic diversity analysis, the nullisome and abnormal chromosome individual identification and the seed purity determination of the Brassica germplasm resources. The core SSR primer composition has the advantages of simpleness in operation, high stability and good reproducibility.

The invention relates to application of a set of SNP loci in screening of populus tomentosa with characters of high photosynthesis and quick growth and a kit, and belongs to the technical field of molecular heredity. According to the application of the set of SNP loci in screening of the populus tomentosa with the characters of high photosynthesis and quick growth, the SNP loci are basic groups in the positions of 2068, 2347 and 2457 of the populus tomentosa PetC gene. Through the application of the SNP loci, quick screening of the populus tomentosa high in photosynthesis and quick in growth can be achieved, and the breeding progress is greatly accelerated.

The method of identifying Date Palm gender using SCAR primers includes using modern genetics techniques for detecting a novel sex-linked marker (SEQ ID NO: 1) in a Date Palm sample. The presence of the Date Palm sex-linked marker (SEQ ID NO: 1) in the sample is indicative that the sample is from a male Date Palm plant. The method of identifying Date Palm gender to determine the gender of Date Palms can be used to determine the gender of Date Palms of any age.

The invention discloses a method for genetic modification of intestinal protobacteria based on microencapsulation treatment. The method mainly includes utilizing the method of synthetic biology to genetically transform the selected intestinal primary escherichia coli and then perform microencapsulation treatment. The biosafety synthetic biology technology is combined with the non-invasive optogenetic technique for the treatment of intestinal diseases, and the method has many advantages compared with the traditional methods for treating the intestinal diseases. The intestinal primary escherichia coli are subjected to genetic modification, and the intestinal colonization compliance of the microencapsulated protobacteria is improved in the later stage. A single functional bioactive molecule,that is, a transforming growth factor (TGF-beta1) secreted by the primary escherichia coli is taken as an example to study the application in treatment of inflammatory bowel disease (IBD). The methodprovides a new idea for studying the interaction between enteric microorganisms and the body.

The invention provides a recombinant Newcastle disease virus (NDV) for expressing a VP3 gene of a new duck parvovirus and application of the recombinant NDV. The ORF of the VP3 gene of the new duck parvovirus is inserted into a full-length transcription vector pLMV-RFP of the NDV by utilizing a reverse genetic manipulation platform of an established NDV PHY.LMV42 strain, so as to construct a recombinant plasmid pLMV-NDPV-VP3, and the recombinant plasmid and three auxiliary plasmids are together transfected with BHK-21 cells to obtain a recombinant vaccine strain rLMV-NDPV-VP3 which is higher in reproductive performance and capable of expressing NDPV-VP3 protein. The NDPV-VP3 gene is located in a non-coding region between a P gene and an M gene of the NDV, and a recombinant virus rLMV-NDPV-VP3 is obtained through reverse genetic technique. The recombinant NDV for expressing the VP3 gene of the new duck parvovirus can be used for preventing duck new parvovirus disease and duck Newcastledisease.

The invention relates to a cloning method of an influenza virus gene, in particular to PCR (polymerase chain reaction) amplification primers of an influenza A virus gene and a rapid cloning method of the influenza A virus gene. According to the PCR amplification primers of the influenza A virus gene and the rapid cloning method of the influenza A virus gene, the PCR amplification primers of eight gene segments of the influenza A virus and primers of linearized influenza virus reverse genetic vectors are provided and the rapid cloning method of the influenza A virus gene is further provided, the influenza virus gene segments and the linearized influenza virus reverse genetic vectors are amplified by the primers through PCR, and then the influenza virus gene segments and the linearized influenza virus reverse genetic vectors are recombined and cloned in vitro by recombinase and transformed to escherichia coli for cloning. According to the invention, the gene cloning process of a traditional influenza virus reverse genetic technology is greatly simplified, and the problems of complex influenza virus gene cloning process and low efficiency of the traditional influenza virus reverse genetic technology are solved.

The invention provides a vaccine strain rSHA-[delta]200, and a construction method and application thereof, relates to that field of influenza vaccine. The vaccine strain rSHA-[delta]200 includes a HAgene that does not contain an NRT glycosylation site. The invention finds out the potential glycosylation sites on the HA protein through analysis, deletes glycosylation sites by site-directed mutagenesis technology, constructs HA expression plasmids with multiple glycosylation sites deleted, obtains recombinant vaccine strains by using 8 plasmid reverse genetic technology, and prepares antiserum. The rSHA-[delta]200 obtained in the invention is an ideal candidate for inactivated vaccine against H9 subtype AIV because of its good cross-immunity and serum cross-neutralization ability.

The invention aims to provide a recombinant canine measles virus expressing luciferase NLuc. The structure of a recombinant CMV nucleic acid fragment for rescuing and expressing NLuc is 5'-N-P-NLuc-M-F-H-L-3', wherein N, P, M, F, H and L are structural genes of the virus; NLuc is ORF of luciferase, the upstream of the ORF contains an M gene initiation sequence and a Not I restriction enzyme cutting site, and the downstream contains a Pme I restriction enzyme cutting site and a P gene termination sequence. The rCMV-NLuc rescued by a reverse genetic technology has a high expression level on NLucin cells, and can emit macroscopic light blue fluorescence under the action of a luminescent substrate furimazine. The insertion of an exogenous gene does not significantly affect the growth kineticsof the virus, and the exogenous gene is relatively stable in the virus passage process.

The method to modify wild NPV and construct the new for Lepidoptera pest with anti-sense- genetic technique is presented and comprises the scheme as: (1) cloning CS conservative sequence of geometrid; (2) constructing the transfer carrier for the anti-sense CS gene of Lepidoptera pest; (3) recombining NPV; (4) validating the NPV with PCR or other methods; (5) contrast with wild NPV, evaluating the insect disinfectation capacity of recombined NPV for screening the virus strain with higher virulence and faster diffusion and secondary infection speed.

The invention provides a mink enteritis parvovirus whole genome infectious clone and a construction method and application thereof. The method comprises the following steps of: carrying out enzyme digestion on a complete genome in an MEV replication form, and sequentially and directionally cloning to a vector pUC18 M in a segmented form to obtain a recombinant plasmid; and mixing the recombinantplasmid with a transfection reagent, and transfecting CRFK cells to obtain a rescue virus. The mink enteritis parvovirus infectious clone constructed by utilizing a reverse genetic technology transfects the CRFK cells in vitro, and can induce the cells to generate cytopathic effects and growth trends the same as those of a parent virus. The method is simple, rapid, time-saving and labor-saving, and has higher accuracy compared with a traditional PCR method, and base mutation can be rapidly and conveniently carried out at any position of the MEV genome by applying the cloning system, so that aneffective way and means are provided for subsequent research and development of molecular biology of MEV and research and development of vaccines.

In the present invention, a recombination of gene groups of nemadectin aglycon biosynthesis is performed for obtaining C-13 hydroxylnemadectin, to which sugar groups can be attached, and a production strain which produces C-13 hydroxylnemadectin is produced. Further, C-13 glycosylnemadectin producing strain is prepared by introducing aveBI-BVIII genes involving glycosidation of avermectin and biosynthesis of oleandrose. As described, C-13 hydroxylnemadectin and C-13 glycosidated nemadectin can be obtained effectively by using the producing strain prepared by means of the molecular genetic technology, and improvement in the biological activity thereof can be expected.

The method of identifying Date Palm gender using SCAR primers includes using modern genetics techniques for detecting a novel sex-linked marker (SEQ ID NO: 1) in a Date Palm sample. The presence of the Date Palm sex-linked marker (SEQ ID NO: 1) in the sample is indicative that the sample is from a male Date Palm plant. The method of identifying Date Palm gender to determine the gender of Date Palms can be used to determine the gender of Date Palms of any age.