5 results about "Genetic Technique" patented technology

The application belongs to the technical field of aquatic genetics, and particularly relates to application of a group of SNP sites in detection of anti-NNV traits of Hippocampus kuda. The application provides a group of molecular marker combinations containing 680 core SNP sites, and sequences of the SNP sites are shown as SEQ ID NO. 1 to SEQ ID NO. 680. The SNP sites are obtained by whole genome resequencing of a reference population of H. kuda, and are screened by using MLE-rank algorithm to be significantly associated with anti-NNV traits. Based on the SNP combinations, the application further provides corresponding liquid phase chips, genomic selection breeding methods and kits. The technology can realize early and accurate evaluation of anti-NNV traits of H. kuda, significantly improve breeding efficiency, reduce detection cost, and provide an effective molecular breeding tool for breeding of H. kuda disease-resistant fine varieties.

The application belongs to the technical field of biology, and particularly relates to a 3D protein mutant O foot-and-mouth disease recombinant virus strain and construction and application thereof. The application mutates methionine at the 403th position of the 3D protein of the O foot-and-mouth disease virus into alanine, and successfully constructs and rescues a 3D protein mutant O foot-and-mouth disease recombinant virus strain rFMDV-M403A by using reverse genetic technology. The amino acid of the recombinant virus strain can be stably mutated in the process of subculture, and the growth speed of the mutant strain is slower than that of the wild strain, and the virus titer is significantly reduced. In individual experiments, the infection of the mutant strain to suckling mice shows that the mutant strain can improve the survival rate of the suckling mice, and significantly reduce the viral load in the liver and the tissue lesions. The application provides an attenuated foot-and-mouth disease recombinant virus strain, which has important significance for in-depth understanding of the pathogenic mechanism and prevention and control strategy of the foot-and-mouth disease virus, and provides an important theoretical basis for further development of the foot-and-mouth disease genetic engineering vaccine.

The application provides a construction method of a recombinant canine distemper virus strain expressing mink parvovirus VP2 protein and relates to the technical field of genetic engineering. The construction method uses a mink canine distemper virus attenuated vaccine CDV3 strain as a basis, clones mink parvovirus VP2 genes into a canine distemper virus genome cDNA, and obtains a recombinant canine distemper virus rCDV3-mVP2 strain capable of simultaneously expressing CDV proteins and MEV VP2 proteins through virus reverse genetic technology, so as to provide a candidate vaccine strain for the research and development of a mink canine distemper and parvovirus enteritis double recombinant live vaccine.

The present invention relates to the field of biological vaccine research and development technology, and in particular, to a VSV vector-based highly pathogenic H5 subtype avian influenza inactivated vaccine and a method for preparing same. The method comprises: using a vesicular stomatitis virus with the deletion of the surface envelope protein gene as a vector, inserting the NA gene and the HA gene of an H5 subtype highly pathogenic avian influenza virus strain between the M gene and the L gene of the vector in the form of an expression cassette, and acquiring a recombinant virus by means of rescue via reverse genetic technology; inoculating the recombinant virus into susceptible cells, culturing for replication and proliferation, and then harvesting a viral solution; inactivating the viral solution to acquire an inactivated viral solution; and adding the inactivated viral solution to a pharmaceutically or veterinarily acceptable carrier, excipient, medium, or adjuvant to acquire an inactivated vaccine. The provided H5 subtype avian influenza inactivated vaccine can induce a high hemagglutination inhibition titer of avian influenza virus on day 21 after chick immunization. The provided inactivated vaccine has good safety and immunogenicity.

The invention discloses a genotype filling optimization method for spruce norvegensis and a reference panel, and belongs to the technical field of forest heredity. The method comprises the following steps: constructing a haplotype reference panel based on large-scale picea norvegensis re-sequencing data; acquiring low-density genotype data; carrying out genotype filling by using IMPUTE5 software and integrating a genetic map of the picea norvegensis; and carrying out quality control on a filling result, and reserving a high-quality SNP site with Rsqsoft greater than or equal to 0.8. According to the method, key parameters and optimal configuration influencing filling accuracy are evaluated and determined for the spruce norveyi system for the first time, and the technical bottleneck that the species are lack of a reliable filling process is solved. Through the method and the special reference panel provided by the invention, high-quality whole genome filling data can be stably and efficiently obtained, and key technical support is provided for genetic research and breeding practice of picea norvegensis.