In-situ degrading bacteria in polycyclic aromatic hydrocarbons polluting soil and identification method of bioremediation capability

A technology for polycyclic aromatic hydrocarbons and contaminated soil, which is applied in biochemical equipment and methods, determination/inspection of microorganisms, and food testing, etc.
CN101034087BInactive Publication Date: 2011-08-31SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
Publication Date
2011-08-31
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

This invention refer to microbe restoring of polycyclic aromatic hydrocarbon ( PAHs) contaminated soil technology region. This invention utilizes phospholipid fatty acid analyzing ( PLFA) technology and stable isotope ratio mass-spectrometric technique ( IRMs) to judge normal position high performance degradation germ in PAHs contaminated soil. The invention takes phospholipid fatty acid as biological tag, combine with stable isotopic tracer technology, base on delta value difference of phospholipid fatty acid abstracted from soil to judge degradation bacterium or florae of PAHs in normal position soil, also can judge given strain whether is autochthon degradation bacterium of contaminated soil. This method can validly combine microbial community architecture with function. This inventionaim at supplying a method of verity reflecting degradation bacterium or florae in PAHs contaminated soil, thereby could analyze selected strain whether or not is autochthon degradation bacterium and its degradation ability.
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Description

technical field

[0001] The present invention relates to the technical field of environmental microbial remediation, specifically using the combination of phospholipid fatty acid analysis (PLFA) technology and stable isotope ratio mass spectrometry (IRMs) to identify in-situ highly efficient degradation bacteria in polycyclic aromatic hydrocarbons (PAHs) contaminated soil and its bioremediation A new approach to capacity. Background technique

[0002] PAHs are a large class of common organic pollutants, mainly found in oil-contaminated soils and rivers, and 16 PAHs have been listed as priority pollutants by the US Environmental Protection Agency (EPA). At present, there are physical methods, chemical methods and biological methods for pollution control. Microbial degradation is generally considered to be one of the most effective means of removing pollutants. In the past, the treatment method relying on the traditional screening of bacteria was a lot of work, with little su...

Claims

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