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Biological chip aldehyde glass carrier

A biochip and aldehydization technology, applied in biochemical equipment and methods, biological testing, microbial determination/inspection, etc., can solve the problems affecting the preparation and overall quality of microarray chips, irregular spotting, and hybridization resolution no problem

Active Publication Date: 2009-07-22
GUANGZHOU DARUI BIOTECH
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  • Abstract
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Problems solved by technology

However, for any of the above surface chemical modification methods, the preparation technology and process of each company are different, and the overall technical method is still immature. The prepared glass slides have high background, insufficient surface uniformity, combined Insufficient power, poor hybridization resolution, and irregular spots, etc., affect the subsequent preparation and overall quality of microarray chips, and hinder the development of microarray chip technology

Method used

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  • Biological chip aldehyde glass carrier
  • Biological chip aldehyde glass carrier

Examples

Experimental program
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Effect test

Embodiment 1

[0018] Example 1: Preparation of microarray chip by spotting on aldylated glass slide

[0019] Such as figure 1 As shown in the present invention, get one piece of aldylated glass slide of the present invention, affix separation fence as the hybridization area, be equipped with HLA-DR4 genotyping oligonucleotide probes (17), and spotting solution components, pass spotting The sampler spots the probes on the glass slide. The probe matrix is, each probe repeats 3 points horizontally, 9 points per row, 6 rows in total. The probe spots prepared by spotting the aldehydized glass slides of the present invention are uniform in size and regular in shape.

Embodiment 2

[0020] Embodiment 2: Check the signal characteristic of microarray chip by hybridization reaction

[0021] Place the chips prepared above overnight, wash with 2×SSC and distilled water for 1 minute each, and dry in the air. Take other components of the HLA-DR4 genotyping reagent, and amplify a DNA sample of known genotype to obtain a hybridization template. Take 2ul of the PCR product and add it to the hybridization area of ​​the chip, then add 8ul of hybridization solution (5×SSC), put the chip into the tank of the automatic hybridization washing machine, set the hybridization temperature to 52°C, and the hybridization time to 40 minutes. Automatic washing and air drying after hybridization.

[0022] Use the GnenPix4100A scanner to scan the hybridization signal to obtain a hybridization result scan (such as figure 2 shown), and convert the images into data, and use analysis software to convert the above data into genotypes. The positive hybridization points and negative h...

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Abstract

The invention relates to a biochip glass slide, in particular to a biochip glass slide prepared by employing an aldehyde group method. The surface of the glass slide is decorated by aldehyde group, thus being convenient for the chemical combination between a nucleic acid probe decorated by amino-group, the antigen-antibody of protein property and other matters. The invention is suitable for the probes of nucleic acids, proteins, and other types, as well as template segment point system microarray chips, has firm and specific chemical bond combining characteristics, and provides the reliable carrier for preparing biochips.

Description

Technical field [0001] The invention relates to a biological chip carrier slide, in particular to a biological chip slide prepared by an aldehyde method. The aldehyde modification on the surface of the glass slide facilitates the chemical combination of amino-modified nucleic acid probes, protein-based antigens, antibodies, and other substances. The invention is suitable for dotting microarray chips with nucleic acid, protein and other types of probes and template fragments, has strong and specific chemical bond combination characteristics, and provides a reliable carrier for the preparation of biochips. Background technique [0002] With its high-throughput, multi-target detection mode, biochips are being increasingly used in the field of biological sciences. Microarray chips based on glass slides have been widely used in basic research, clinical screening, and auxiliary diagnosis due to their high efficiency, high throughput, and low price. [0003] Microarray chips incl...

Claims

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Application Information

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IPC IPC(8): C03C17/00C03C21/00C12Q1/68G01N33/53G02B21/34
Inventor 胡守旺张帆李明程钢何蕴韶
Owner GUANGZHOU DARUI BIOTECH
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