Magnetic bead time resolution immunofluorescence quantitative detection H-FABP (heart-type fatty acid binding protein) kit

A time-resolved fluorescence, H-FABP technology, used in fluorescence/phosphorescence, measurement devices, analytical materials, etc., can solve the problems of long detection time, low sensitivity, poor stability, etc., to achieve simple and fast operation, high quantum yield, good stability

Inactive Publication Date: 2018-06-19
GUANGZHOU BIOKEY HEALTH TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, methods for detecting H-FABP mainly include enzyme-linked immunoassay, fluorescent immunochromatography, and chemiluminescence, but these methods have disadvantages such as low sensitivity, long detection time, high cost, and poor stability. For early diagnosis and treatment monitoring, it is necessary to provide a simple, fast, accurate and reliable detection reagent and detection instrument

Method used

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  • Magnetic bead time resolution immunofluorescence quantitative detection H-FABP (heart-type fatty acid binding protein) kit
  • Magnetic bead time resolution immunofluorescence quantitative detection H-FABP (heart-type fatty acid binding protein) kit
  • Magnetic bead time resolution immunofluorescence quantitative detection H-FABP (heart-type fatty acid binding protein) kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] In this embodiment, the magnetic bead time-resolved fluorescence immunoquantitative detection kit for H-FABP consists of reagent strips and H-FABP calibrator.

[0029]Wherein, the shape of the reagent strip is fan-shaped, and 1 to 13 holes are arranged sequentially from left to right. The 1st and 2nd holes are test holes, the 3rd and 4th holes are fluorescent marker holes, the 6th and 7th holes are cleaning solution holes, the 8th and 9th are sample diluent holes, the 12th are enhancement solution holes, the 5th and 10th holes are , 11,13 are preparatory holes. A magnet can be stored between the 1st and 2nd wells for magnetic separation experiments. The 1st and 2nd wells can store a liquid volume of 300 μl; the 3rd and 4th wells can be disassembled from the entire reagent strip to become independent components, which is convenient for fluorescent markers. Pack and store. The 3rd, 4th, and 5th wells can store a liquid volume of 400 μl; the 6th and 7th wells can store a...

Embodiment 2

[0059] The preparation method of the H-FABP calibrator is: dilute the H-FABP antigen to 0, 1, 5, 10, 50, 100 , 160ng / ml. The making and detection of embodiment 2 reagent strips

[0060] The semi-finished reagent strips in this example are assembled through the following procedures: 50 μL of H-FABP immunomagnetic beads, 200 μL of europium-labeled H-FABP antibody, 2000 μL of washing solution, 2000 μL of Washing solution, 400 μL enhancement solution, and then seal with a film sealing machine. The sealing film is coated with product information identification that can be scanned and recognized by an automatic fluorescence detection analyzer, including enterprise standard curve, batch, production date, and expiration date. The finished reagent strips, the packaged H-FABP calibrator and other accessories are assembled into a kit.

[0061] Sample testing:

[0062] ①Add sample:

[0063] Put the sample or calibrator to be tested into the loading system of the automatic instrument, ...

Embodiment 3

[0074] The comparison of embodiment 3 and commercially available kit

[0075] Adopt commercially available heart-type fatty acid-binding protein assay kit (immunoturbidimetric method) (Padding Bioengineering (Beijing) Co., Ltd.) and the method detection sample in embodiment 1 (in the described sample, the concentration range of H-FABP is 2.5 ~160ng / mL) was repeated three times to verify the correctness of the detection results of the kit of the present invention, and the results are shown in Table 1 below.

[0076] Table 1 Sample test results

[0077]

[0078] Compared with commercially available reagents, the deviation of this reagent is less than ±10%, and the detection result of this reagent is accurate and reliable.

[0079] The commercially available heart-type fatty acid binding protein assay kit (immunoturbidimetric method) (Padin Bioengineering (Beijing) Co., Ltd.) and the reagents in Example 1 were used to detect 200 clinical samples, and the test results are show...

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Abstract

The invention discloses a magnetic bead time resolution immunofluorescence quantitative detection H-FABP (heart-type fatty acid binding protein) kit. The kit comprises an H-FABP monoclonal antibody-coated immunomagnetic bead, an H-FABP calibration product solution, a europium-marked H-FABP monoclonal antibody solution, cleaning liquid and an enhancement solution. The H-FABP monoclonal antibody-coated immunomagnetic bead is super paramagnetic bead with functional group modification and the diameter of 1 to 3 [mu]m and an H-FABP monoclonal antibody covalent conjugate. The kit is high in sensitivity, the sensitivity of H-FABP is 2 ng/mL, and a serum (plasma) sample does not need to be diluted; the detection time is short and a report can be output within 30 minutes; the demand quantity of samples is small and one-time sample loading only needs 50 [mu]L; the matched fully-automatic time resolution immunoanalyzer is simple in operation and labor-saving.

Description

technical field [0001] The invention relates to the technical field of in vitro reagent detection, in particular to a magnetic bead time-resolved fluorescence immunoquantitative detection kit for H-FABP. Background technique [0002] Acute Coronary Syndrome (ACS) includes acute myocardial infarction (AMI) and unstable angina (UA), and its morbidity and mortality are high. Perfusion therapy, the mortality and prognosis will be significantly improved. Therefore, it is of great significance to select early markers with high specificity to improve the accuracy of early diagnosis and timely treatment for ACS patients. Myoglobin (MYO), troponin (troponin I, cTnI or troponin T, cTnT) and creatine kinase isoenzyme (creatine kinasemass, CK-MB) are currently commonly used clinical biochemical markers of myocardial injury. Among them, MYO appears earlier, but its specificity is poor; while CK-MB and cTnI have lower sensitivity and appear later in the early stage of ACS. Heart-Type F...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N33/577G01N33/533G01N33/543
CPCG01N21/6486G01N33/533G01N33/54326G01N33/577
Inventor 李根平
Owner GUANGZHOU BIOKEY HEALTH TECH CO LTD
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