Virus expression veclor of tomato flower-leaf virus low virulent strain K

Abstract

The present invention relates to the field of plant virus gene engineering technology. It utilizes gene insertion recombination mode to make the cDNA genome of low virulent strain K of tomato mosaic virus with low virulent protecting function implement recombination to construct recombinant virus vector capable of high-effectively and stably expressing exogenous gene, after it is transcripted into RNA, it can directly infect plant or use transformed agrobacterium to infect plant to obtain recombinant virus. Said recombinant virus is separated and purified, then can be inoculated into host plant again, and can continuously and high-effectively express exogenous gene in plant body for several generations. The target protein expressed by natural form can be made into oral preparation.

Description

Technical field [0001] The invention relates to the field of plant virus genetic engineering, in particular to a virus expression vector of the tomato mosaic virus attenuated strain K of the tobacco mosaic virus genus and a construction method thereof. Background technique [0002] The construction of expression vectors using plant viruses began in the 1980s. In 1980, Shepherd et al. discovered that the DNA clone of cauliflower mosaic virus (CaMV) can infect plants by mechanical inoculation to have the characteristics of an expression vector; after obtaining the cDNA clone of plant RNA virus infectivity, it was also used for development Expression vector (Ahlguist et al., 1983; Kumagai et al., 2000). In the past ten years, the use of plant viruses as vectors to express foreign proteins has received considerable attention, and some RNA virus vectors have made considerable progress. (Hendy et al., 1999; McCormick et al., 1999; Gopinath et al., 2000; Zhang et al., 2000). [0003] Pl...

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Problems solved by technology

So far, plant virus vectors are all constructed with strong viruses, so they cannot be used to improve the traits of agricultural crops or increase their nutritional and medicinal value. The main reason for this is that most of the known plant viruses can cause host plant yields significant drop in

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