3results about How to "Avoid restrictive" patented technology

The application discloses a Pincer ligand rare earth metal catalyst and a preparation method thereof, and application of the Pincer ligand rare earth metal catalyst in preparation of primary amine borane compounds and secondary amine compounds. The Pincer ligand rare earth metal catalyst can be bonded with a nitrogen atom in a borane ammonia complex, thereby activating a B-H bond, so that the preparation of the primary amine borane compounds and the secondary amine compounds can be completed by using the borane ammonia complex. Compared with pinacol borane (HBpin), the borane ammonia complex (H3NBH3) has the characteristics of high stability, small toxicity and convenient storage as a boron source, and can reduce energy consumption and be environment-friendly in the reaction. In addition, the Pincer ligand rare earth metal catalyst has the advantages of simple source, small dosage and low preparation difficulty.

The present application belongs to the field of biology and chemistry, and relates to a method for visual detection of porcine pseudorabies virus (PRV) by using a CRISPR / Cas12a system. ‑ / TK ‑ The present application discloses a crRNA for differentiating a CRISPR / Cas12a-based visual porcine pseudorabies wild strain and gE ‑ / TK ‑ gene deletion vaccine strain, and corresponding primers and probes. The differential diagnosis method of the present application comprises the following steps: step one, extraction of a virus genome; step two, amplification of the extracted virus genome by using a constant temperature amplification primer through a constant temperature amplification technology; and step three, use of the amplified product for Cas12a detection. The method of the present application is simple to operate and has superior sensitivity and specificity.

This application relates to a management method for a power battery pack, belonging to the field of vehicle manufacturing technology. The method includes: for each cell in the power battery pack to be tested, performing AC internal resistance deviation diagnosis, capacity decay diagnosis, and voltage change rate trajectory anomaly diagnosis on the cell, obtaining the cell's AC internal resistance value, internal resistance anomaly judgment value, cell capacity estimate, capacity anomaly judgment value, and voltage cumulative anomaly judgment value, respectively. Based on the internal resistance anomaly judgment value, capacity anomaly judgment value, and voltage cumulative anomaly judgment value, the cell is determined to be faulty; the number and location of faulty cells are counted; if the number of faulty cells is less than or equal to a preset maximum allowable short-circuit number, the faulty cells are short-circuited based on their location, and the system parameters of the short-circuited power battery pack are reconstructed; if the number of faulty cells is greater than the maximum allowable short-circuit number, the power battery pack to be tested is replaced. Using this application can improve the accuracy of faulty cell identification.