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Bisphenol A molecularly imprinted electrochemical sensor and preparation method and application thereof

A molecular imprinting and electrochemical technology, applied in the field of electrochemical detection, can solve the problems of poor recognition of target molecules, long antibody preparation time, large operating errors, etc., and achieve easy promotion, good reproducibility and stability, and wide linear range Effect

Inactive Publication Date: 2016-10-12
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this type of instrument detection technology has the disadvantages of expensive, time-consuming operation, and the need for professional skills to operate; while immunoassay methods such as enzyme-linked immunoassay (ELISA) and fluorescence polarization immunoassay (FDIA) have strong recognition performance and high sensitivity. , but there are also disadvantages such as long antibody preparation time, poor recognition of target molecules with similar structures, and large operational errors.

Method used

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  • Bisphenol A molecularly imprinted electrochemical sensor and preparation method and application thereof
  • Bisphenol A molecularly imprinted electrochemical sensor and preparation method and application thereof
  • Bisphenol A molecularly imprinted electrochemical sensor and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A kind of preparation method of gold and reduced graphene oxide nanocomposite material:

[0030] Weigh 20 mg of graphene oxide, ultrasonically disperse in 20 mL of water for 1 hour, add 9.6 μM HAuCl 4 , the final volume of the solution is 40mL, magnetically stirred for 12 hours overnight; then heated to 80°C in an oil bath, continued to stir and added 60μL of hydrazine hydrate, and reacted at constant temperature for 3 hours; stopped the reaction, continued to stir and cooled to room temperature; centrifuged A black solid was obtained and vacuum-dried to obtain a gold and reduced graphene oxide nanocomposite product. Its reaction process is as figure 1 shown. The obtained product was identified by scanning electron microscopy, and the results were as follows: image 3 As shown in A and B, the modified reduced graphene oxide has gold nanoparticles on its surface.

Embodiment 2

[0032] A method for preparing a molecularly imprinted electrochemical sensor:

[0033] (1) Use 0.3μm and 0.05μm Al on the glassy carbon electrode in sequence 2 o 3 The powder was polished on suede, and after cleaning, it was ultrasonically washed in absolute ethanol and ultrapure water, and then dried in a beaker.

[0034] (2) Weigh 5 mg of the gold obtained in Example 1 and the reduced graphene oxide nanocomposite material, and ultrasonically disperse them in DMF / water (volume ratio 1:1) for 1.5 hours to prepare a dispersion.

[0035] (3) Take 5 μL of the dispersion obtained in step (2) with a pipette gun, drop it on the surface of the electrode, dry it in an oven at 50°C, rinse it with ultrapure water and dry it in the air to obtain Au / rGO / GCE.

[0036] (4) Au / rGO / GCE is used as the working electrode, the platinum sheet is used as the counter electrode, and saturated calomel is used as the reference electrode to form a three-electrode working electrolytic cell; the three e...

Embodiment 3

[0039] Using the bisphenol A molecularly imprinted electrochemical sensor obtained in Example 2 to detect bisphenol A comprises the following steps:

[0040] The bisphenol A molecularly imprinted electrochemical sensor was placed in a bisphenol A phosphate buffer solution (pH 6 .9), after soaking for 15 minutes, take it out and rinse it with ultrapure water, put it into the electrolytic cell for differential pulse voltammetry scanning, the scanning potential is 0.3-0.8V, the potential increment is 0.004V, and the pulse width is 0.05V , with an amplitude of 0.2V. After each scan, the bisphenol A molecularly imprinted electrochemical sensor was placed in the blank bottom solution for cyclic scanning until there was no peak, rinsed with water and dried, and the operation was repeated. Its operation diagram is as figure 2 As shown, the result is as Figure 5 , the detection linear range of the molecularly imprinted electrochemical sensor to bisphenol A is 2.50×10 -7 M-1.75×10...

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Abstract

The invention belongs to the electrochemical detection technical field, and discloses a molecularly imprinted electrochemical sensor for detecting bisphenol A and a preparation method and an application thereof. A glassy carbon electrode modified by a gold and reduced graphene oxide nano composite material is used as a working electrode, a poly-o-phenylenediamine and bisphenol A mixed film is prepared by cyclic voltammetry, and finally, the bisphenol A is eluted to obtain the molecularly imprinted electrochemical sensor. The content of bisphenol A in a soil sample is detected by differential pulse voltammetry, and compared with a high performance liquid chromatography method, the methods have good correlation. The imprinted electrochemical sensor has the characteristics of fast response, high sensitivity, strong selectivity, wider linear range, high practicability, easy popularization and the like.

Description

technical field [0001] The invention belongs to the technical field of electrochemical detection, in particular to a molecularly imprinted electrochemical sensor for detecting bisphenol A, a preparation method and application thereof. Background technique [0002] Bisphenol A (BPA) is an important organic chemical raw material synthesized from phenol and acetone in an acidic medium. During the manufacturing process of hundreds of daily necessities. Studies have proved that bisphenol A has endocrine disrupting effects, mainly manifested in the effect of simulating estrogen, and even a very low dose can cause animals to have precocious puberty, decreased sperm count, and prostate hyperplasia. In addition, it has biological toxicity, such as embryotoxicity and teratogenicity, which can significantly increase the occurrence of cancers such as ovarian cancer, prostate cancer, and leukemia in animals. [0003] There are a variety of analysis and testing methods for bisphenol A, ...

Claims

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Application Information

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IPC IPC(8): G01N27/48
CPCG01N27/48
Inventor 丁金龙刘洁赵肃清崔锡平吴盼盼胡青青焦洛莹陈莹珊沈定
Owner GUANGDONG UNIV OF TECH
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