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Preparation method of electrode for detection of dopamine (DA)

A dopamine and electrode technology, which is applied in the field of biochemical analysis, can solve the problems of complicated electrode preparation and expensive glassy carbon electrodes, and achieves the effects of good selectivity, low preparation cost and high sensitivity

Inactive Publication Date: 2014-07-09
HOHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the electrodes used in these electrochemical detection DA methods are glassy carbon electrodes, which are relatively expensive and require complex pretreatment and activation steps, making the preparation of electrodes very complicated.

Method used

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  • Preparation method of electrode for detection of dopamine (DA)
  • Preparation method of electrode for detection of dopamine (DA)
  • Preparation method of electrode for detection of dopamine (DA)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1, Preparation of SPE / rGO / β-CD / GOx

[0039] Activation treatment of SPE: In the electrochemical workstation, the reference electrode of SPE is used as the reference electrode, the counter electrode of SPE is used as the counter electrode, and the working electrode of SPE is used as the working electrode. Scan the 0.5M sulfuric acid solution at a scanning speed for 10 cycles. The activated electrodes were left to dry at room temperature.

[0040] Add 260 mg of GO into 130 ml of absolute ethanol and ultrasonically disperse until GO is completely dispersed in absolute ethanol to obtain a yellow-brown homogeneous liquid. Add 50ml of hydroiodic acid with a mass fraction of 55% to 58% to the dispersed GO, and heat in a water bath at a constant temperature of 85°C for 1h. After the reaction, the solution was centrifuged and dried to obtain rGO.

[0041] 100 mg of rGO was sonicated in 100 ml of absolute ethanol for 3 h until rGO was completely dispersed to obtain ...

Embodiment 2

[0044] Example 2 , SPE / rGO / β-CD / GOx electrode DA detection sensitivity, detection limit and detection range

[0045] Prepare DA solutions of 5, 10, 15, 20, 30, 40, 50, 60, 65, 70, 80, and 100 μM respectively, and use the reference electrode of SPE / rGO / β-CD / GOx prepared in Example 1 as The reference electrode, the counter electrode of SPE / rGO / β-CD / GOx was used as the counter electrode, and the working electrode of SPE / rGO / β-CD / GOx was used as the working electrode, and the 12 different concentrations were scanned by differential pulse voltammetry (DPV) DA solution, the scanning potential range is -0.2 ~ 0.6V. Take the peak flow value of DA at each concentration and the DA concentration to make a fitting curve.

[0046] Figure 6 The fitting curve of the peak current value and DA concentration of different concentrations of DA scanned by DPV method for SPE / rGO / β-CD / GOx electrode ( I (DA)=-0.0788 C (DA)-7.8173). According to the graph analysis, the linear range of SPE / rGO / ...

Embodiment 3

[0047] Example 3 Selective detection of DA by SPE / rGO / β-CD / GOx under the interference of UA

[0048] Mixtures of DA and UA with DA concentrations of 5, 10, 20, 30, 40, 50, and 55 μM were prepared respectively, wherein the concentration of UA was always 10 times the concentration of DA. According to the DPV method adopted in Example 2, the detection limit, sensitivity and linear range of the SPE / rGO / β-CD / GOx electrode to DA were obtained in the case of UA interference.

[0049] Figure 7 When the UA interference concentration is 10 times the DA concentration, the SPE / rGO / β-CD / GOx electrode scans the fitting curve of the DPV peak current value and the DA concentration of different concentrations of DA ( I (DA)=-0.0596 C (DA)-2.6134). According to the graph analysis, under UA interference, the linear range of SPE / rGO / β-CD / GOx electrode to DA is 5-55μM, and the sensitivity is 0.0596μAμM -1 , the limit of detection was 2.06 μM. It shows that the electrode has high selectivi...

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Abstract

The invention discloses a preparation method of an electrode for detection of dopamine (DA). The preparation method of the electrode comprises the following steps: dispensing reduction graphene oxide (rGO) onto the screen-printed electrode (SPE) surface to obtain SPE / rGO, using a cyclic voltammetry method for electrolytic deposition of a beta-cyclodextrin (beta-CD) and glucose oxidase (GOx) mixture onto the SPE / rGO electrode to obtain an modified electrode SPE / rGO / CD / GOx. The preparation method has the advantages of low cost and simple steps, and the prepared electrode is fast in response speed, high in sensitivity and good in selectivity.

Description

technical field [0001] The invention belongs to the field of biochemical analysis, and relates to a preparation method of a fast electrode for detecting dopamine. Background technique [0002] Dopamine (DA) is an important neurotransmitter in the central nervous system, which can regulate various functions and mental activities of the central nervous system in the brain, such as perception, emotion, behavior and movement. DA imbalance in the body will lead to diseases such as schizophrenia and Parkinson's syndrome. Therefore, the determination of its content is of great significance in both physiological function research and clinical application. [0003] In recent years, the methods and applications of different types of detection of DA have become a research hotspot. The methods commonly used at home and abroad to detect DA include high performance liquid chromatography, ultraviolet-visible spectrophotometry, fluorescence spectrophotometry, capillary electrophoresis, an...

Claims

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Application Information

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IPC IPC(8): G01N27/327
Inventor 华祖林白雪秦琴徐露竹顾莉褚克坚程浩淼
Owner HOHAI UNIV
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