62results about How to "Short measurement period" patented technology

The method comprises: sampling coal core from underground mining; at underground mining, measuring and calculating the gas loss quantity of coal core; at the ground, measuring and calculating the desorption of the coal mining gas; measuring and calculation the grind gas desorption of partial coal core; calculating the content of gas capable of being desorbed.

The invention relates to an apparatus and method for measuring elastic turbulence characteristics of a viscoelastic polymer in a porous medium, wherein a side connector of a fluorescent microscope of the apparatus is connected to a CCD (couple charged device) camera, a saline container, a polymer solution container and a synthetic oil container that are connected with an oil / water container are parallelly connected between an inlet of a micro metering pump and an inlet of a two-dimensional mesh microscopic simulation model, the two-dimensional mesh microscopic simulation model is provided with an inlet pressure sensor and an outlet pressure sensor, and a light guide arm of a micro field particle image speed-measuring system is positioned right above the two-dimensional mesh microscopic simulation model; all the micro field particle image speed-measuring system, the fluorescent microscope, the inlet pressure sensor and the outlet pressure sensor are connected to a data graphic data station. The apparatus and method are capable of effectively recognizing elastic turbulence of viscoelastic polymer solution in porous medium seepage, and providing qualitative and quantitative description for elastic turbulence characteristics.

The invention relates to a device and a method for carrying out on-line detection of the biochemical oxygen demand (BOD) in a water body sample by utilizing a microbiological fuel cell. The detection device has the advantages of real-time and on-line continuous detection function, simple operation, long continuous working time, and lower use and maintenance costs. The upwelling microbiological fuel cell is taken as the core, a BOD sample enters the microbiological fuel cell directly or after being diluted, and a linear relation exists between the coulomb yield of the microbiological fuel cell and the BOD content of the sample, thereby obtaining the BOD content of the sample through detecting the electrical signal generated by the microbiological fuel cell and through analyzing the obtained data by a computer control system. The detection method includes detecting the oligotrophic BOD concentration with the BOD concentration value in the sample less than critical value, and the eutrophic BOD concentration with the BOD concentration value in the sample larger than critical value; the detection of the eutrophic BOD concentration can be completed by adopting a buffer solution dilution method or a pulse integration method.

The invention discloses a determination method for iron, aluminum, manganese, calcium, titanium, silicon and magnesium in casting powder. The method comprises the following steps: melting a test sample, which is subjected to pre-carbon removal treatment, with a sodium carbonate-boric acid mixed flux; dissolving a leached and cooled melted block with hydrochloric acid and heating at low temperature to decompose the melted block; diluting to a specified volume; taking one part of the solution, atomizing the solution and introducing the atomized solution into an inductive coupling plasma optical emission spectrometer; measuring relative intensity of an element to be detected in the solution to a yttrium internal standard element at a selected wavelength; calculating the mass percent of each element to be detected according to a calibration curve manufactured by a standard solution. The determination method provided by the invention is an ICP (Inductively Coupled Plasma) light spectrum method and the content of various components in the casting powder is determined in one step; the determination method has an accurate and reliable measured result and is simple to operate; the dosage of reagents is greatly reduced and the analysis speed is improved by 70 percent or more, so that a determination period is greatly shortened, and comprehensive utilization of the casting powder and requirements of actual working in a production field are met.

The invention discloses a method for measuring blood concentration of paraquat. The method comprises the following steps of: (1) pretreating a sample, namely adding aqueous solution of an internal standard substance and a protein precipitation agent acetonitrile into a plasma sample, performing whirl mixing, centrifuging and sampling supernatant; (2) separating the sample, namely adopting a universal C18 liquid chromatographic column, and using an ion pairing agent in an acid mobile phase, wherein the acid mobile phase is mixed solution of 3mmol.L<-1> aqueous solution of sodium dodecyl sulfate, 0.2 percent of aqueous solution of trifluoroacetic acid, acetonitrile and water; and (3) detecting by using a diode array detector, namely detecting by using the diode array detector at a detectionwavelength of 250 to 260nm, measuring peak areas of the internal standard substance and the paraquat, and calculating the blood concentration of the paraquat through least square method linear regression. The plasma sample is easy and convenient to pretreat, the detection process is sensitive and rapid, toxic substances and the blood concentration thereof can be rapidly determined in actual application, and the method is high in clinical application value.

The invention relates to a method for measuring tin elements, comprising the following steps of: deoxidating a sample; dissolving with the mixed acid of hydrochloric acid and nitric acid; adjusting acidity of the solution with ammonia water and the hydrochloric acid; quantificationally complexing the tin and other elements capable of complexing with EDTA with excessive EDTA; with xylenol orange as an indicator, titrating excessive EDTA with the standard titration solution; buffering with hexamethylene tetramine to ensure that the pH value is between 5 and 6; adding sodium fluoride for separating out EDTA which is equivalent to the amount of the tin; titrating separated EDTA with the standard titration solution until the solution becomes red from yellow; and calculating the percentage of the tin according to the volume of the standard titration solution consumed in the second titration. The method has the recovery rate of 95.54-104.76% and the relative standard deviation of 1.41-2.67%. The reagents used in the method are all common chemical reagents, and no special devices are needed. In addition, the method has the advantages of simple process, short measurement period and low cost.

The invention provides a method for detecting a phthalate plasticizer in an inner packing material of medicine. The method comprises the following steps: weighing the inner packing material of medicine in a measuring flask, adding methanol for dilution, filtering with a filter membrane, and taking a subsequent filtrate as liquid for testing; respectively weighing dimethyl phthalate, diethyl phthalate and dibutyl phthalate in a measuring flask, adding a methanol solvent to a scale for dilution, and taking a solution as a contrast liquid 1; weighing dioctyl phthalate in a measuring flask, adding a methanol solvent to a scale for dilution, and taking a solution as a contrast liquid 2; injecting the liquid for testing, the contrast liquid 1 and the contrast liquid 2 in order for detection by a HPLC, and finishing the detection of the phthalate plasticizer in the inner packing material of medicine. The method can be used for distinguishing whether a plasticizer component is added into the common packing materials of medicines, and an accurate quantitative analysis is carried out, so that product quality which is influenced by migration of plasticizer components of medicine coating powder and packing materials to medicine is controlled.

The present invention provides a method for detecting a nucleic acid by specific binding between ligand and receptor, in particular, a method of detecting a SNP by the specific binding between a ligand and receptor. The present invention also provides a method for detecting a nucleic acid that is simpler, requiring only a single measurement operation, and shorter in measurement period. The present invention also provides a highly sensitive and highly accurate method for detecting a nucleic acid by using a specific binding reaction between a ligand and receptor in a reaction at the interface of a liquid and solid. The present invention also detects a nucleic acid by using a coagulation reaction of dispersible particles.

The invention relates to a dilution device for determination of a high-concentration water sample through a water-quality on-line analyzer. The dilution device comprises a dilution sample pipe, wherein the dilution sample tube is communicated with a peristaltic pump A and a sample feeding pipe; a pipe clamping valve A is arranged at the communication part of the dilution sample pipe and the sample feeding pipe; a discharging pipe is arranged on the dilution sample pipe; a pipe clamping valve B is arranged at the top end of the discharging pipe of the dilution sample pipe; a pipeline is communicated with the side wall of the dilution sample pipe; an optical detector is arranged on the pipeline of the side wall of the dilution sample pipe; a peristaltic pump B is communicated with the pipeline of the side wall of the dilution sample pipe; a peristaltic pump C is communicated with the inlet of the sample feeding pipe; an overflow pipe is arranged on the sample feeding pipe; a two-position three-way electromagnetic valve is arranged between the peristaltic pump B and the pipeline of the side wall of the dilution sample pipe. The dilution device has the advantages that the dilution ratio is high, on-line monitoring is realized, and the dilution precision is high.

The invention discloses a method and device for rapidly determining the gas content of a coal seam, and belongs to the technical field of coal mine safety. A crushing device is adopted, and in the process of taking a coal sample underground, the time from coal drilling to loading of the coal sample into the crushing device, temperature and atmospheric pressure parameters are recorded through a pressure sensor; after well lifting, the crushing device filled with coal samples is installed on a weighing device for crushing, the weights of the coal samples in different stages are weighed through the weighing device, the gas density serves as a bridge, the gas volume is calculated through the weight change value, and the content of desorbed gas in the crushing process is calculated; the problems that an existing gas content measuring technology is complex in method and high in time cost are solved. The device is simple and convenient to operate, high in practicability, time-saving, labor-saving, low in cost and capable of quickly and accurately measuring the coal seam gas content.

The invention provides a method for determining electrode reaction parameters. The method comprises the following steps: accessing three electrodes of a three-electrode system reaction device to an electrochemical workstation, measuring initial data by utilizing a cyclic voltammetry, processing the initial data to obtain electrochemical reaction parameters and data relationship graph; processing the initial data, namely, firstly reading files in batch, storing the read data by adopting a four-layer data structure, reading the information of temperature and scanning rate from a file name, correcting and preprocessing the data after all data is read and stored, drawing according to a result after the correction, judging the reaction type, and combining a computational formula to obtain the diffusion coefficient, reaction activating energy and rate constant data, and finally displaying the calculation result and graph. By adopting the method, the reaction kinetics and the thermodynamics parameters of the electrode can be conveniently measured, the determination period is greatly shortened, the overall workload is alleviated, the overall price is low, and the final calculation result and drawn graph can meet the majority of requirements.

The invention provides a pretreatment method for directly measuring trace cadmium in soil by a solid sample introducing atomic fluorescence cadmium analyzer, and belongs to the field of analysis of trace pollutants in the environment. The method comprises the following steps: putting test soil which is ground through a ball mill and filtered by a 100-mesh sieve into a sample boat, adding absolute ethanol and a saturated potassium permanganate solution for soaking, and directly testing trace cadmium in the soil by the solid sample introducing atomic fluorescence cadmium analyzer. According to the method, the detection sampling amount is low; no complicated sample digestion process is required, so that the sample pollution and the element loss can be avoided; the test soil is directly obtained, absolute ethanol and KMnO4 are added into the test soil, the reaction is instantly finished, and the time for the whole one-time test process does not exceed 3 minutes, so that the test cycle is shortened; the method has the characteristics of high accuracy and sensitivity. The invention provides a new effective pretreatment method for quick field detection analysis of trace heavy metal cadmium in the soil.

The invention discloses a HPLC (High Performance Liquid Chromatography) analysis method for sodium 3,3'-dithiodipropane sulfonate and belongs to the technical field of analysis. The HPLC analysis method is characterized in that a high performance liquid chromatograph with an ultraviolet detector is used for determining; octadecyl silane is taken as a filling column, the size of a chromatographic column is 250mm (length)*4.6mm (inner diameter) and the grain size is 5 microns; methyl alcohol and a buffer solution in volume ratio of (0-50):(100-50) is taken as a flowing phase; the detection wavelength is 190-330nm, and the column temperature is at 10-60 DEG C; the speed of the flowing phase is 0.3-1.2mL / min; the feeding size of a sample is 20 micro liter; the buffer solution contains 0.17mol / L acetic acid, 0.142mol / L triethylamine and ultrapure water. The content of sodium 3,3'-dithiodipropane sulfonate in a to-be-detected sample can be quickly and accurately measured according to a standard curve established via a standard solution; the determination result error is small; the repeated operability is strong; the precision is less than 2.8%; the recovery rate is 96.7%-99.3%; the HPLC analysis method can be widely applied to the production process detection and product quality detection for industrial sodium 3,3'-dithiodipropane sulfonate.

The invention provides an underground coal mine coal seam gas-bearing capacity and pressure rapid measuring instrument and measuring method, the underground coal mine coal seam gas-bearing capacity and pressure rapid measuring instrument comprises a main machine and a coal sample tank, the main machine comprises a measuring pipeline, a flow sensor, a display screen, a keyboard, a battery and a charging data sharing serial port and a controller, the flow sensor is set on the measuring pipeline, one end of the measuring pipeline is connected to the coal sample tank through a conveying pipeline,the controller is in signal connection with the flow sensor, the display screen, the keyboard and the charging data sharing serial port, and the battery is in connection with the charging data sharingserial port. The underground coal mine coal seam gas-bearing capacity and pressure rapid measuring instrument has the advantages of convenient operation and strong practicability, coal seam gas pressure P and coal seam true gas content W can be accurately measured in a site of an underground coal mine within 10 min only by sieving of 50 g of a coal sample of 1 to 3 mm, the measurement period of the coal seam gas pressure P and the coal seam true gas content W can be greatly shortened, and the principle and the method of the coal seam gas pressure and the coal seam gas content can be revolutionized.

The invention discloses a monoclonal cell strain. The monoclonal cell strain is a human renal epithelial 293T cell carrying an SIE-regulated luciferase gene and capable of expressing luciferase stably and is named as 293T-SIE (preservation No. CGMCC No. 13816, and the depositary unit is the General Microbiology Center of the China General Microbiological Culture Collection Center); the invention further relates to a construction method and application for the monoclonal cell strain and a method for measuring relative biological activity of IL-6R inhibitors by adopting the monoclonal cell strain. According to the Monoclonal cell strain and application for measuring relative biological activity of the IL-6R inhibitors, the activation situation of IL-6 related signal pathway is taken as a research object, a human renal epithelial cell strain capable of expressing SIE-luciferase reporter gene stably is designed, culturing is simple, repeatability is good, actual action situation of sample in a body is closer, and stability and accuracy of the measuring result are guaranteed; meanwhile, the established method for measuring the activity of the IL-6R inhibitor established is short in measuring period, researching and developing, quality controlling and clinical application of IL-6R inhibitor medicine are facilitated, and high application value is obtained.

The invention belongs to the technical field of medical detection systems, and particularly discloses a conveying system for quickly rechecking a specimen. The system comprises a workbench and a conveyor belt, the workbench is provided with a waiting area and a rechecking track leading to the waiting area, the waiting area comprises a mounting plate and a mounting table, and a push plate is slidably arranged at the end, close to the waiting area, of the rechecking track. A first driving unit for driving the push plate to slide back and forth along the mounting table is arranged on the mountingtable. A baffle is arranged between the push plate and the mounting table, a first elastic unit is arranged between the baffle and the mounting table, and a sliding groove is formed in the end, closeto the waiting area, of the rechecking track. A movable plate is slidably arranged in the sliding groove, and a second elastic unit is arranged between the side, away from the waiting area, of the sliding groove and the movable plate. A second driving unit for driving the movable plate to push the to-be-rechecked specimen to a centrifugal chamber is arranged on the workbench. By means of the system, the to-be-rechecked specimen can be rapidly conveyed, and the conveying period is shortened.

The embodiment of the invention provides a substrate moisture absorbing-holding characteristic measuring device and a using method thereof. According to the method, a holding force pressure-stabilizing module is used for generating a stable negative pressure. A substrate moisture sensing module includes a substrate storage and drainage unit and a moisture sensor; the substrate storage and drainageunit is used for placing a test substrate; and the moisture sensor is used for measuring the water content of the test substrate and sending the water content to the processing module. A holding force adjusting module enables the suction forces of the test substrate and the holding force pressure-stabilizing module to be equal. The test substrate can reach a preset water flow by adjusting the negative pressure generated by a holding force balance unit; the holding force balance unit is used for enabling the test substrate to keep the preset water flow. The processing unit calculates a moisture absorbing-holding characteristic curve of the test substrate in an optimized manner. According to the invention, the measuring device has advantages of simple operation, low cost and the like; the loose structure of the substrate is not protected; and thus the basic technical staff or production front line staff can promote and apply the device conveniently.

The invention provides a method for measuring content of silicon dioxide in gold ore and belongs to the field of analytical chemistry. The method comprises steps as follows: after a sample is subjected to alkali fusion, leaching and acidification, potassium chloride and potassium fluoride are added to form potassium fluosilicate precipitates with silicon ions, potassium fluosilicate is hydrolyzed in hot water, hydrofluoric acid with isoequivalent weight is separated out, and content of silicon dioxide is measured by adopting phenolphthalein as an indicator and a sodium hydroxide standard solution for titration. The method for rapidly and accurately measuring the content of silicon dioxide in gold ore is established, the measuring period is short, the operation is simple, degree of precision and degree of accuracy are high, and the blank in the field of analysis for silicon dioxide in gold ore is filled up.

The invention discloses a method for cleaning reactants and a sample analyzer. According to the method for cleaning the reactants, a mixing chamber and a measuring chamber which controllably communicate with each other are provided, when the measuring chamber and the mixing chamber are cleaned, a replacement type cleaning mode is adopted, so that a sample in the measuring chamber is replaced by a cleaning liquid firstly, and then the cleaning fluid in the measuring chamber is replaced by a diluent, so that cleaning is completed, the mixing chamber also has the similar condition, wherein a sample in the mixing chamber is replaced by the cleaning liquid firstly, and then the cleaning fluid in the mixing chamber is replaced by the diluent, so that cleaning is completed, therefore, the problem that the dilution concentration of the cleaning fluid needs to be accurately controlled so as to prevent the influence caused by the dilution concentration on the cleaning result is solved, accordingly, in the whole cleaning process of the method, the process of diluting the cleaning liquid is also avoided, so that the whole measuring period is directly shortened, and the measuring speed is increased.

The invention discloses a method for rapidly determining lithium oxide, potassium oxide and sodium oxide in continuous casting mold fluxes. The method comprises the following steps: 1) performing decomposition treatment on the continuous casting mold fluxes; 2) preparing a mold flux sample solution; 3) preparing a standard solution of to-be-determined components; 4) drawing a calibration curve; and 5) determining the content of each component in the mold flux sample. The method disclosed by the invention has the advantages of being wide in linearity range, high in accuracy, simple in operation, rapid, sensitive, small in usage amount of reagents and the like, laboratory contamination can be reduced, the determination period is shortened by using a plasma spectrometer, and the requirementson incoming inspection of the continuous casting mold fluxes and on analysis of actual application in the production field can be met.

The invention discloses a precise fertilizing system which comprises a sample feeding device, a nutrient detection device, a control device and a fertilizing device. The sample feeding device, the nutrient detection device and the fertilizing device are all connected with the control device, the sample feeding device is used for sucking sample feeding liquid for reaction, the sample feeding liquidcomprises a nitrogen reagent, a phosphorus reagent, a potassium reagent, a nutrient solution and a diluents, the nutrient detection device is connected with the sample feeding device and used for color comparison of reaction liquid to obtain absorbance, the control device is used for controlling flow-in of the sample feeding liquid in the sample feeding device and adjusting wavelength of a lightsource in the nutrient detection device and further used for calculating concentration of fertilizer in the sample feeding liquid according to the absorbance and sending a fertilizing signal to the fertilizing device according to the concentration of the fertilizer, and the fertilizing device is used for fertilizing according to the fertilizing signal. The precise fertilizing system can realize in-situ automatic quick monitoring of nutrients and realize precise fertilizing according to nutrient monitoring data.

The invention relates to the technical field of chlorella pyrenoidosa, and provides a method for fluorescence detection of biotoxicity of atrazine by using chlorella pyrenoidosa, the biotoxicity of atrazine is detected by using chlorella pyrenoidosa under the condition of vegetable oil, the vegetable oil is methylated and ethylated vegetable oil, and the content of the methylated and ethylated vegetable oil is less than 1%. Comprising the following steps: taking atrazine standard solutions with a series of concentrations, adding a vegetable oil solution, then adding into a chlorella pyrenoidosa solution, uniformly mixing, controlling the volume ratio of the chlorella pyrenoidosa solution to the vegetable oil solution to the atrazine standard solution to be 1: (1-2): (1-2), darkly placing for 10 minutes, measuring each chlorophyll fluorescence kinetic parameter of the chlorella pyrenoidosa, and calculating the chlorophyll fluorescence kinetic parameter of the chlorella pyrenoidosa according to the chlorophyll fluorescence kinetic parameter of the chlorella pyrenoidosa. And determining the biotoxicity of atrazine. According to the technical scheme, the problem that a method for detecting the biotoxicity of atrazine in the prior art is long in determination period is solved.

The invention discloses a measuring device for influences of different fertilizers on same plant root system. The measuring device comprises a culture vessel. A baffle plate is fixed on the inner wallof the culture vessel, and the baffle plate divides the interior of the culture vessel into three equal parts; a hollow section is provided as an observation area for the influences of the differentfertilizers on the same plant root system after the baffle plate divides into three equal parts; placing holes are formed in the center position of the culture vessel by a sterile hole punch; culturemediums embedded with plant seeds are contained inside the placing holes; circular holes formed by the sterile hole punch are provided at the bottom surface of the observation area; fertilizer cakes made of the different fertilizers are placed inside the circular holes. Individual differences in the test, interferences of the fertilizers and a medium are avoided, so that the experiment result is more accurate; meanwhile, the growth condition of the plant root system can be monitored in real time due to the fact that the culture mediums are transparent; the method can be applied to the solid fertilizers and the liquid fertilizers at the same time, is a good innovation scheme, and has a good market popularization prospect.

The invention relates to a method for determining a silver content in crude copper, which belongs to the field of analytical chemistry. According to the invention, lefort aqua regia is used for digestion of a sample, hydrochloric acid is added for complexation of silver in the crude copper, and a flame atomic absorption spectrometry is used for determining the silver content. The method uses two reagents of the hydrochloric acid and nitric acid for digestion of the sample, the reagent is saved, and the experiment cost is reduced; the experiment steps are simple, and are easily grasped by inspectors, and are adapted to detection of large-batch samples; the accuracy is high, and the precision is good. The method avoids the poisonous and harmful medicines such as lead oxide, health of the inspectors can be protected, a purpose of protecting environment can be achieved, the method provides technical support and guarantee for the crude copper industry analysis tests, and popularization andapplication of the method in the industry can be promoted, the method has the advantages of high accuracy, short determination period, and environmental protection, and achieves a purpose of reducingcost and improving effect for enterprises.

The method comprises: sampling coal core from underground mining; at underground mining, measuring and calculating the gas loss quantity of coal core; at the ground, measuring and calculating the desorption of the coal mining gas; measuring and calculation the grind gas desorption of partial coal core; calculating the content of gas capable of being desorbed.

The invention discloses a determination method for storage life of emulsion explosive. The determination method comprises the following steps: step one, carrying out high and low temperature cycle experiments for known emulsion explosive, and then measuring the detonation velocity of the known emulsion explosive changing with number of cycles till the detonation velocity reaches a critical value 3,200m.s-1; step two, measuring the detonation velocity of the known emulsion explosive changing with number of storage days under natural storage conditions; step three, establishing a corresponding relation graph of the detonation velocity changing with the number of high and low temperature cycles and the number of storage days for the known emulsion explosive; step four, measuring a detonation velocity value after high and low temperature cycles of an explosive sample to be evaluated, and determining the number of cycles corresponding to the detonation velocity value; and step five, finding out the storage time of the explosive to be evaluated under natural conditions through the corresponding relation graph obtained in step three. The determination method disclosed by the invention selects the detonation velocity with small influence factors of experimental environment and manual operation as an index of detonation property so as to enable measured data to be stable and reliable, and high in accuracy, thus being a rapid and accurate method for detecting the storage life of the emulsion explosive.

The invention relates to a coal seam interior gas parameter measuring probe and a using method thereof. The coal seam interior gas parameter measuring probe comprises a conical head, a pressure measuring pipe, a first probe main pipe and a plurality of second probe main pipes, wherein the conical head, the pressure measuring pipe, the first probe main pipe and the multiple second probe main pipes are detachably connected in sequence from left to right. During construction of the coal seam interior gas parameter measuring probe, the workload is few, due to extruding of a coal body and the probe, the self-sealing effect can be achieved after the probe is nailed into the coal body for a certain distance, it is guaranteed that the real gas pressure of the position where the probe pressure measuring pipe is located can be measured, and the time needed for measuring is short. According to the coal seam interior gas parameter measuring probe and the using method thereof, the gas pressure measuring period can be shortened, the pressure measuring success rate can be increased, the destroyed types of coal can further be judged through the nailing difficult and easy level in the process that the measuring probe is knocked into the coal body to provide a basis for gas relevant parameter measuring, and the coal seam interior gas parameter measuring probe and the using method thereof are high in practicability and worthy of being popularized.

The present invention discloses a method for rapidly determining the reaction performance of a dissolving pulp, and relates to the field of pulp preparation-papermaking and textile. According to the invention, cellulose precipitated from a viscose liquid is rapidly digested and oxidized by potassium dichromate, the absorbance of the solution after the reaction is determined by an ultraviolet spectrophotometer, and finally the mass of the cellulose participating in the yellowing reaction is calculated through mathematical conversion, such that the reaction performance of the dissolving pulp is rapidly determined; and with the method, the influence of the related factors is reduced, the stability of the dissolving pulp reaction performance determination result is increased, and the problems of complex operation process, long determination period and poor repeatability of the traditional dissolving pulp reaction performance determination method are solved.

The invention relates to a primer and method for recognizing zoysia japonica varieties. The nucleotide sequences of the primer are shown as the SEQ ID No.1 and the SEQ ID No.2. The invention further provides a method for recognizing the zoysia japonica varieties. The method comprises the steps that a genome DNA of a sample to be tested is used as a template DNA, and PCR amplification is carried out by means of the primer. Compared with an existing three-nature testing and recognizing method (namely the appearance, morphological characteristic description method) for recognizing zoysia japonica varieties, testing results are not affected by environment factors, operation is convenient and rapid, the testing period is obviously shortened, and the testing results are more accurate and reliable.