Method for fluorescence detection of biotoxicity of atrazine by using chlorella pyrenoidosa

A technology of chlorella pyrenoidosa and atrazine, which is applied in the direction of fluorescence/phosphorescence, measuring devices, and material analysis through optical means, can solve the problems of long measurement period, achieve short measurement period, improve the impact effect, The effect of increasing the contact area

Active Publication Date: 2022-05-06
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

[0005] The present invention proposes a method for detecting the biotoxicity of atrazine by using the fluorescence of Chlorella pyrenoidosa, which solves the problem of long measurement period of the method for detecting the biotoxicity of atrazine in the related art

Method used

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  • Method for fluorescence detection of biotoxicity of atrazine by using chlorella pyrenoidosa
  • Method for fluorescence detection of biotoxicity of atrazine by using chlorella pyrenoidosa
  • Method for fluorescence detection of biotoxicity of atrazine by using chlorella pyrenoidosa

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preparation example Construction

[0056] 3. Preparation of Chlorella pyrenoidosa liquid

[0057] The logarithmic phase of Chlorella pyrenoidosa obtained during the cultivation of Chlorella pyrenoidosa species, that is, the initial value OD during the transfer process 680 =0.1 Culture and growth in the incubator, then select OD 680 =1.0 means that the algae cell density is obtained through microscope inspection, and the cell density range is (1-1.2)×10 6 The concentration of Chlorella pyrenoidosa liquid ind / ml is used as the concentration of algae for detecting the biotoxicity of atrazine, and is used for the determination of the biotoxicity of atrazine.

[0058] 4. Preparation of atrazine standard solution:

[0059] 20000μg / L atrazine stock solution: take 1ml of atrazine standard solution with a concentration of 100μg / mL, add deionized water to dilute to 5mL, and obtain atrazine stock solution;

[0060] 200μg / L atrazine intermediate solution: take 2.0mL atrazine stock solution in a 100mL volumetric flask, a...

Embodiment 1

[0065] Determination of the effect of atrazine on the fluorescence effect of Chlorella pyrenoidosa:

[0066] In the logarithmic phase of Chlorella pyrenoidosa growth (ie OD 680 = 1.0) Take Chlorella pyrenoidosa liquid, atrazine standard solution of series concentration and culture medium to mix and shake evenly according to the volume ratio of 1:2:1, and set up a blank group at the same time (medium replaces Atrazine Razin) for comparison. After the mixture was placed in the dark for 10 min, the kinetic parameters of chlorophyll fluorescence in Chlorella pyrenoidosa liquid were measured with a water sample chlorophyll fluorescence meter, including the maximum light energy conversion efficiency (Fv / Fm) of photosystem II, the actual light intensity of photosystem II For energy conversion efficiency (Y(II)), photosynthetic electron transfer efficiency (ETR), photochemical quenching (qP) and non-photochemical quenching (QN), three parallel control experiments were carried out, an...

Embodiment 2

[0071] In the logarithmic phase of Chlorella pyrenoidosa growth (ie OD 680 = 1.0) Take Chlorella pyrenoidosa liquid, atrazine standard solution of series concentration and culture medium to mix and shake well at a volume ratio of 1:1:1, and set up a blank group at the same time (medium replaces Atrazine Razin) for comparison. After the mixture was placed in the dark for 10 min, the kinetic parameters of chlorophyll fluorescence in Chlorella pyrenoidosa liquid were measured with a water sample chlorophyll fluorescence meter, including the maximum light energy conversion efficiency (Fv / Fm) of photosystem II, the actual light intensity of photosystem II For energy conversion efficiency (Y(II)), photosynthetic electron transfer efficiency (ETR), photochemical quenching (qP) and non-photochemical quenching (QN), three parallel control experiments were carried out, and the average value was taken and recorded to obtain a blank The changes of each fluorescence parameter in the exper...

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Abstract

The invention relates to the technical field of chlorella pyrenoidosa, and provides a method for fluorescence detection of biotoxicity of atrazine by using chlorella pyrenoidosa, the biotoxicity of atrazine is detected by using chlorella pyrenoidosa under the condition of vegetable oil, the vegetable oil is methylated and ethylated vegetable oil, and the content of the methylated and ethylated vegetable oil is less than 1%. Comprising the following steps: taking atrazine standard solutions with a series of concentrations, adding a vegetable oil solution, then adding into a chlorella pyrenoidosa solution, uniformly mixing, controlling the volume ratio of the chlorella pyrenoidosa solution to the vegetable oil solution to the atrazine standard solution to be 1: (1-2): (1-2), darkly placing for 10 minutes, measuring each chlorophyll fluorescence kinetic parameter of the chlorella pyrenoidosa, and calculating the chlorophyll fluorescence kinetic parameter of the chlorella pyrenoidosa according to the chlorophyll fluorescence kinetic parameter of the chlorella pyrenoidosa. And determining the biotoxicity of atrazine. According to the technical scheme, the problem that a method for detecting the biotoxicity of atrazine in the prior art is long in determination period is solved.

Description

technical field [0001] The invention relates to the technical field of chlorella pyrenoidosa, in particular to a method for detecting the biological toxicity of atrazine by utilizing the fluorescence of chlorella pyrenoidosa. Background technique [0002] Atrazine (Simetryn) is a s-triazine herbicide with a chemical name of 2-methylthio-4,6-bis(ethylamino)-1,3,5-triazine and a molecular formula of C8H15N5S. It is a systemic conduction type selective pre-emergence soil treatment agent developed by Geigy in 1959. It can be absorbed through the roots and leaves of weeds, and then transmitted to the whole body of the plant, inhibiting the photosynthesis of weeds, so as to achieve Weeding purposes. [0003] Atrazine is suitable for field weeding of rice, corn, soybean, wheat, peanut, cotton and other crops. It is a systemic selective herbicide. It can be absorbed and transmitted through the roots and leaves. The whole plant has a special effect on the vicious weeds of rice fie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 崔建升王柳卜马小龙
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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