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Method for establishing electrochemical immunosensor for detecting carcino-embryonic antigen

A technology of immunosensor and construction method, which is applied in the field of electrochemical immunosensor, and can solve problems such as long analysis time, poor biocompatibility, and radiation hazards

Inactive Publication Date: 2015-05-13
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these methods are sensitive and reliable, there are some disadvantages: radiation hazards, long analysis time, complex electrical instruments, etc.
However, the biocompatibility of pure silver nanomaterials is not good, so it is necessary to prepare materials with high catalytic activity and good biocompatibility to construct enzyme-free sensors.

Method used

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  • Method for establishing electrochemical immunosensor for detecting carcino-embryonic antigen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Prepare an electrochemical immunosensor for detecting CEA standard samples.

[0039] Step 1. Preparation of PDDA-functionalized GR

[0040] a Preparation of graphene oxide: Add 100 mL of a mixed solution of phosphoric acid and concentrated sulfuric acid with a volume ratio of 1:9 into a three-neck flask, then weigh 0.75 g of graphite powder and add it to the above solution, heat to 30 °C, and add to the solution After adding 4.5 g of potassium permanganate, adjust the temperature of the water bath to 50 °C, and react for 12 h under magnetic stirring conditions. After the reaction stops, pour the sample into 300 mL of ice water with 4 mL of hydrogen peroxide added in advance, and stir thoroughly to obtain The mixture was separated by centrifugation, washed 7 times with ultrapure water, and then dried in a vacuum oven at 60 °C to obtain a brown-yellow graphene oxide solid;

[0041] b Preparation of GR: Dissolve 25 mg of the graphene oxide prepared in the above ...

Embodiment 2

[0054] Example 2 Prepare an electrochemical immunosensor for detecting serum samples containing CEA.

[0055] Step 1. Preparation of PDDA-functionalized GR

[0056] a Preparation of graphene oxide: Add 100 mL of a mixed solution of phosphoric acid and concentrated sulfuric acid with a volume ratio of 1:9 into a three-neck flask, then weigh 0.75 g of graphite powder and add it to the above solution, heat to 30 °C, and add to the solution After adding 4.5 g of potassium permanganate, adjust the temperature of the water bath to 50 °C, and react for 12 h under magnetic stirring conditions. After the reaction stops, pour the sample into 300 mL of ice water with 4 mL of hydrogen peroxide added in advance, and stir thoroughly to obtain The mixture was separated by centrifugation, washed 7 times with ultrapure water, and then dried in a vacuum oven at 60 °C to obtain a brown-yellow graphene oxide solid;

[0057] b Preparation of GR: Dissolve 25 mg of the graphene oxide prepared in th...

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Abstract

The invention relates to a method for establishing an electrochemical immunosensor for detecting carcino-embryonic antigen, in particular to a method for establishing an electrochemical immunosensor for detecting carcino-embryonic antigen based on a graphite / bovine serum albumin stabilized silver nanoparticle (GR / Ag@BSA) composite material. The method is characterized by comprising the following steps: firstly, depositing a layer of gold nanoparticles on the surface of a glassy carbon electrode by using a potential deposition method, sequentially immobilizing primary antibodies and antigen on the surface of the electrode, and finally immobilizing a second antibody solution marked by GR / Ag@BSA through specific reaction of antigen antibodies. The gold nanoparticles are excellent in conductivity, the used GR / Ag@BSA composite material not only integrates excellent conductivity of GR and good biocompatibility of BSA, but also is high in catalytic activity to hydrogen peroxide reduction, and the electrochemical immunosensor which is established based on the material is relatively high in detection sensitivity on carcino-embryonic antigen and relatively in detection limit.

Description

technical field [0001] The invention belongs to the technical field of electrochemical immunosensors, and in particular relates to a construction method of an electrochemical immunosensor for detecting carcinoembryonic antigens. Background technique [0002] Carcinoembryonic antigen (CEA) is a broad-spectrum tumor marker, which can reflect the existence of a variety of tumors, and is a good tool for judging the curative effect, disease development, monitoring and prognosis of colorectal cancer, breast cancer and lung cancer. tumor markers. Currently, immunoassay methods for detecting CEA mainly include radioimmunoassay, enzyme-linked immunoassay, piezoelectric immunoassay, and fluorescence immunoassay. Although these methods are sensitive and reliable, there are some disadvantages: radiation hazards, long analysis time, complex electrical instruments, etc. [0003] In recent years, electrochemical immunosensors have received extensive attention due to their inherent advant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N27/26G01N33/532
CPCG01N33/57473G01N27/3275G01N33/5438
Inventor 于京华孙国强张彦葛慎光颜梅刘海云杨红梅马超
Owner UNIV OF JINAN
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