Method for employing double indicatrix immunochromatography by semi-quantity to diagnose carcinoembryonic antigen

A technology of immune chromatography and carcinoembryonic antigen, applied in the field of bioengineering, can solve the problems of distinguishing benign and malignant tumors, and achieve the effect of easy promotion and application, high accuracy and single detection object

Inactive Publication Date: 2011-10-19
正元盛邦(天津)生物科技有限公司
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  • Abstract
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Problems solved by technology

However, the clinical standard of 5ng/mL alone cannot directly and quickly distinguish benign and malignant tumors, so it is necessary to develop semi-quantitative

Method used

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  • Method for employing double indicatrix immunochromatography by semi-quantity to diagnose carcinoembryonic antigen
  • Method for employing double indicatrix immunochromatography by semi-quantity to diagnose carcinoembryonic antigen
  • Method for employing double indicatrix immunochromatography by semi-quantity to diagnose carcinoembryonic antigen

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Experimental program
Comparison scheme
Effect test

Embodiment

[0048] 1. Prepare CEA monoclonal antibodies 1 and 2. And clean the glassware required for the experiment.

[0049] 2. Preparation of colloidal gold

[0050] First heat the 0.02% chloroauric acid solution to boil, then quickly add 2% trisodium citrate 2mL;

[0051] The color of the solution changed from light blue to dark blue. Continue heating to turn into wine red, continue to boil for 10 minutes; turn to transparent wine red, stop heating and continue to stir to room temperature. In this way, a 20 nm colloidal gold solution was prepared. Then use an electron microscope to check to ensure that the prepared gold particles are as consistent and uniform in size as possible, and the particle diameter is about 20nm, otherwise it will be remade.

[0052] 3. Preparation of colloidal gold markers

[0053] Take two 1.5mL test tubes, add 1mL colloidal gold solution respectively; add an appropriate amount of borax buffer solution to adjust the pH to 8.8; add 20μg / mL CEA monoclonal ant...

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Abstract

The invention discloses a detection method in the technical field of biological engineering and relates to a method for employing double indicatrix immunochromatography by semi-quantity to diagnose carcinoembryonic antigen in particular. The carcinoembryonic antigen is a broad-spectrum tumor marker, can reflect the existences of multi-tumors to people and is a better tumor marker for curative effect judgment, disease development, monitoring and preoperative prognosis on colorectal cancer, breast cancer and lung cancer. Clinic detection on CEA (carcinoembryonic antigen) is usually served as a detection criterion for the early aided diagnosis and cancer postoperation recrudescence of colon cancer, gastric cancer, esophagus cancer, rectal cancer, etc. At present, common CEA detection methods on clinic detection comprise ELISA (enzyme-linked immuno sorbent assay), CLIA and so on, waste time and labor, is not beneficial to popularization and has high cost. The technology of immune collaurum develops rapidly and is applied widely to the detection on infectious diseases, early pregnancy, cancer and the like. The method adopts the collaurum immune chromatography to build a method for quickly detecting the CEA, and can detect the range of a CEA gray area from 5ng/mL-20ng/mL with semi quantity. The CEA can be taken as the basis for differential diagnosis of benign and malignant tumors.

Description

technical field [0001] The invention relates to a detection method in the technical field of bioengineering, in particular to a method for semi-quantitative diagnosis of carcinoembryonic antigen by double indicator line immunochromatography. Background technique [0002] Malignant tumors have become a major disease that endangers human health, and the incidence of malignant tumors of the digestive system ranks first. According to relevant domestic clinical surveys, the incidence of malignant tumors of the digestive system such as colorectal cancer, colon cancer, liver cancer and pancreatic cancer has increased in the population in recent years, and malignant tumors of the digestive system account for about 60-70% of all tumors, ranking first First, it is also "top" in the "ranking list" of mortality, and it is showing a younger trend. Therefore, the importance of carrying out research on the diagnosis and treatment of digestive system tumors is self-evident. This shows that...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N21/78G01N33/52G01N33/532
Inventor 霍五奎岳晓燕马雪明刘寅王晶罗云萍张有青
Owner 正元盛邦(天津)生物科技有限公司
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