Method for employing double indicatrix immunochromatography by semi-quantity to diagnose carcinoembryonic antigen
A technology of immune chromatography and carcinoembryonic antigen, applied in the field of bioengineering, can solve the problems of distinguishing benign and malignant tumors, and achieve the effect of easy promotion and application, high accuracy and single detection object
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[0048] 1. Prepare CEA monoclonal antibodies 1 and 2. And clean the glassware required for the experiment.
[0049] 2. Preparation of colloidal gold
[0050] First heat the 0.02% chloroauric acid solution to boil, then quickly add 2% trisodium citrate 2mL;
[0051] The color of the solution changed from light blue to dark blue. Continue heating to turn into wine red, continue to boil for 10 minutes; turn to transparent wine red, stop heating and continue to stir to room temperature. In this way, a 20 nm colloidal gold solution was prepared. Then use an electron microscope to check to ensure that the prepared gold particles are as consistent and uniform in size as possible, and the particle diameter is about 20nm, otherwise it will be remade.
[0052] 3. Preparation of colloidal gold markers
[0053] Take two 1.5mL test tubes, add 1mL colloidal gold solution respectively; add an appropriate amount of borax buffer solution to adjust the pH to 8.8; add 20μg / mL CEA monoclonal ant...
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