Method for semi-quantitatively diagnosing alpha-fetoprotein by using double-indicatrix immunochromatography

A technology of immunochromatography and alpha-fetoprotein, applied in the field of bioengineering, can solve problems such as rapid diagnosis of liver cancer, and achieve the effects of easy promotion and application, fast detection speed, and single detection object

Inactive Publication Date: 2012-01-18
正元盛邦(天津)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, clinical abnormalities of liver cells and liver cancer cannot be distinguished, and the standard of 20ng / mL alone cannot directly and quickly diagnose liver cancer. Therefore, a semi-quantitative colloidal gold test strip that can detect AFP 20-400ng / mL, which is the gray area of ​​AFP diagnosis, was developed. is also very necessary
At present, there are no relevant test strips for sale at home and abroad

Method used

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  • Method for semi-quantitatively diagnosing alpha-fetoprotein by using double-indicatrix immunochromatography
  • Method for semi-quantitatively diagnosing alpha-fetoprotein by using double-indicatrix immunochromatography
  • Method for semi-quantitatively diagnosing alpha-fetoprotein by using double-indicatrix immunochromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0047] 1. Prepare AFP monoclonal antibodies 1 and 2. And clean the glassware needed for the experiment.

[0048] 2. Preparation of colloidal gold

[0049] First heat the 0.02% chloroauric acid solution to boil, then quickly add 2% trisodium citrate 2mL;

[0050] The color of the solution changed from light blue to dark blue. Continue heating to turn into wine red, continue to boil for 10 minutes; turn to transparent wine red, stop heating and continue to stir to room temperature. In this way, a 20 nm colloidal gold solution was prepared. Then use an electron microscope to check to ensure that the prepared gold particles are as consistent and uniform in size as possible, and the particle diameter is about 20nm, otherwise it will be remade.

[0051] 3. Preparation of colloidal gold markers

[0052] Take two 1.5mL test tubes, add 1mL colloidal gold solution respectively; add an appropriate amount of borax buffer solution to adjust the pH to 8.8; add 20μg / mL AFP monoclonal antib...

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Abstract

The invention discloses a detection method in the technical field of biological engineering, in particular to a method for semi-quantitatively diagnosing an alpha-fetoprotein (AFP) by using a double-indicatrix immunochromatography. The alpha-fetoprotein is a protein generated by embryonic cells and a yolk sac and is peculiar to a fetus. The content of the AFP in blood serum of a normal adult person is extremely low (less than 20ng / ml), and when hepatic cells cancerize, the content is greatly increased (larger than 400ng / ml), therefore the detection of abnormal increase of the content of the AFP in the blood serum is a very effective basis for clinical judgment of early discovery and diagnosis of primary liver cancer. At present, the clinically used AFP detection methods are ELISA (Enzyme Linked Immunosorbent Assay), CLIA (Chemiluminescent Immunoassay) and other methods, which are time-consuming and labor-wasting, are not favorable for popularization and have expensive costs. An immune colloidal gold technique develops fast in recent years and is widely applied to the detection of communicable diseases, early pregnancy, cancer and the like. In the invention, a colloidal gold immunochromatography is adopted, and the method for quickly detecting the AFP is established, and an AFP gray zone range of 20ng / mL-400ng / mL can be semi-quantitatively detected.

Description

technical field [0001] The invention relates to a detection method in the technical field of bioengineering, in particular to a method for semi-quantitative diagnosis of alpha-fetoprotein by double indicator line immunochromatography. Background technique [0002] Hepatocellular carcinoma (HCC) is one of the most common malignant tumors in my country, and it is the third most common malignant tumor in terms of mortality after gastric cancer and esophageal cancer. Liver cancer has a high degree of malignancy and develops rapidly. The average age of the disease is 44 years old. If the treatment is not timely or the treatment plan is improperly selected, the average survival time is half a year. About 110,000 people die from liver cancer every year in my country, accounting for 45% of the world's liver cancer deaths. However, with the wide application of AFP and other detection methods, the incidence and detection rate of liver cancer are gradually increasing. This shows that ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/577G01N33/574G01N33/558
Inventor 霍五奎岳晓燕马雪明刘寅王晶罗云萍张有青
Owner 正元盛邦(天津)生物科技有限公司
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