Preparation method of paper-based electrochemical luminescence converter and applications of paper-based electrochemical luminescence converter in carcino-embryonic antigen detection

A carcinoembryonic antigen and electrochemical technology, applied in the field of preparation of three-dimensional paper-based converters, can solve problems such as poor conductivity and lack of target recognition elements, and achieve the effects of simple preparation process, high accuracy of measurement results, and low cost

Inactive Publication Date: 2016-12-21
UNIV OF JINAN
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Problems solved by technology

[0005] In order to solve the problems of poor conductivity and lack of specific target recognition elements in the process of protein detection, and further expand the detection limit of tumor markers, we introduced molecular transformation technology into the detection of tumor markers, and transformed the input protein signal In order to output the signal of the aptamer chain, combined with the cheapness, portability, and easy modification of the paper chip, the detection of low-concentration tumor markers can be achieved by means of electrochemiluminescence.

Method used

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  • Preparation method of paper-based electrochemical luminescence converter and applications of paper-based electrochemical luminescence converter in carcino-embryonic antigen detection
  • Preparation method of paper-based electrochemical luminescence converter and applications of paper-based electrochemical luminescence converter in carcino-embryonic antigen detection
  • Preparation method of paper-based electrochemical luminescence converter and applications of paper-based electrochemical luminescence converter in carcino-embryonic antigen detection

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Embodiment 1

[0028] Preparation of paper-based electrochemiluminescence converter and its application in the detection of carcinoembryonic antigen:

[0029] (1) Design the batch printing pattern of the hydrophobic wax of the paper-based electrochemiluminescence converter on the computer, which are respectively defined as paper chip 1 and paper chip 2;

[0030] (2) Use a wax printer to print a batch pattern of hydrophobic wax on A4 size paper chip 1 and paper chip 2, and then place the paper chip printed with the batch pattern of hydrophobic wax in an oven and heat until the wax melts, making it on the paper chip. to form a hydrophobic region;

[0031] (3) The reference electrode and the counter electrode printing pattern are sequentially printed on the hydrophilic area on the left side of the front side of the paper core 1 obtained in step (2) by the method of screen printing, wherein the right side of the front side is the hydrophilic working area, The left side of the front side is the ...

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Abstract

The invention discloses a preparation method of a paper-based electrochemical luminescence converter and applications of the electrochemical luminescence converter in carcino-embryonic antigen detection. Through wax printing technology, screen printing technology and the functionalization of paper chips, an input signal of protein is converted into an output signal of a specific aptamer chain to further cause the change of luminol electrochemical luminescence intensity, so that the high-sensitive and portable detection of low-concentration tumor markers can be realized.

Description

technical field [0001] The invention relates to the technical field of electrochemiluminescence analysis and detection, in particular to the preparation of a three-dimensional paper-based converter suitable for electrochemiluminescence analysis. Background technique [0002] Under the action of carcinogenic factors in normal cells, intracellular genes will be changed, and cell growth regulation will be changed accordingly, which will lead to abnormal monoclonal hyperplasia and cause cancer of normal cells. In recent years, the incidence and mortality of cancer have been increasing day by day, and people are full of fear and concern about it. Therefore, early warning of cancer has become a major research goal of scientific researchers. [0003] Tumor markers are a class of substances that reflect the presence or absence of tumors, and their presence and quantitative changes can provide the properties of tumors. Therefore, the detection of tumor markers plays an important role...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N33/574
CPCG01N21/76G01N33/57473
Inventor 李丽张彦黄煜真梁琳琳葛慎光颜梅于京华
Owner UNIV OF JINAN
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