Time resolution fluorescence method comprehensive detection pancreatic cancer kit and application thereof

A time-resolved fluorescence, comprehensive detection technology, applied in the field of biological and medical detection technology, can solve the problems of far different judgment results, errors in detection results, and many detection steps, and achieves fewer steps, shorter time, and improved specificity. and sensitivity effects

Inactive Publication Date: 2013-06-12
河南生生医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Conventional laboratories or medical units usually use a single kit for the detection of these four indicators, that is, one kit for the detection of one tumor marker. The disadvantages of this method are: 1. There are many detection steps, which are time-consuming and laborious; 2. , At present, the four markers are detected by four single-index kits, and then comprehensively judged. Due to differences in kits from different manufacturers, and different kits from the same manufacturer may have control due to different markers detected. Differences in conditions and parameters lead to errors in the detection results, which will have a greater impact on the specificity and sensitivity of the comprehensive analysis of the four markers, and may lead to very different judgment results

Method used

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  • Time resolution fluorescence method comprehensive detection pancreatic cancer kit and application thereof
  • Time resolution fluorescence method comprehensive detection pancreatic cancer kit and application thereof
  • Time resolution fluorescence method comprehensive detection pancreatic cancer kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The time-resolved fluorescence method comprehensive detection kit for pancreatic cancer provided in this example includes four monoclonal antibodies: anti-CA242 antibody C3918, anti-CA50 antibody AM09238PU-N, anti-CA199 antibody ab3982, and anti-CEA antibody MAM02-008 Composition of mixed antibody-coated microplates; with Eu 3+ Labeled C3624 antibody (anti-CA242 antibody), with Tb 3+ Labeled AM09239PU-N antibody (anti-CA50 antibody), with Sm 3+ Labeled ab116024 antibody (anti-CA199 antibody), with Dy 3+ Labeled MAM02-881 antibody (anti-CEA antibody) mixed antibody composed of four monoclonal antibodies; analysis buffer; common fluorescence enhancer; washing solution; quality control composed of four standard mixtures of CA242, CA50, CEA, and CA199 Taste. The kit also includes negative controls, positive controls, substrates and other related reagents.

[0034] In the kit provided in this example, anti-CA242 antibodies C3918 and C3624 were self-made; anti-CA199 antib...

Embodiment 2

[0047] The application of the kit provided in Example 1 of the present invention in the comprehensive detection of pancreatic cancer tumor markers CA242, CA50, CEA, and CA199, that is, the kit provided in Example 1 of the present invention is used to comprehensively detect pancreatic cancer tumor markers CA242, CA242, and CA199. CA50, CEA, CA199 time-resolved fluorescent immunoassay method, the reaction principle is as follows figure 1 As shown, the double-antibody sandwich method is used, including the following steps:

[0048] (1) Dilute the four protein standard mixtures of CA242, CA50, CEA, and CA199 with the analysis buffer, that is, the quality control product, and make a mixed solution with a series of concentration gradients containing the four protein standards of CA242, CA50, CEA, and CA199 ;

[0049](2) Take 100 μl human serum of the sample to be tested and the mixed solution of four protein standards prepared in step (1), add the mixed antibody-coated antibody com...

Embodiment 3

[0051] The numerical values ​​of CA242, CA50, CEA, and CA199 four indexes obtained by testing 40 serum samples with the test kit of the present invention and the same 40 serum samples are respectively tested with the single-index kit of CanAg Company for CA242 and Shenzhen New Industry Company. Regression correlation analysis was performed on the data obtained from the single-index kit test CA50, the single-index kit test CA199 of Shenzhen New Industry Company, and the single-index kit test CEA of Abbott Company, respectively, corresponding to the attached Figure 2-5 , as can be seen from the accompanying drawings, each index data obtained by the test kit of the present invention has a good correlation with the data obtained by the test of the single index kit respectively.

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Abstract

The invention relates to the technical field of biology and medical science detection, in particular to a detection kit of comprehensive detection pancreatic cancer tumor markers crux armature (CA) 242, carcinoma embryonic antigen (CEA), CA 50, CA 199 in the same reaction system and further relates to the application for detecting pancreatic cancer tumor markers CA 242, CEA, CA 50, CA 199 in the kit. The diction kit comprises micro-well plates and mixed antibodies and the like, wherein the micro-well plates coats respectively a first monoclonal antibody mixed antibodies for preventing CA 242, CEA, CA 50, CA 199, the mixed antibodies of a second monoclonal antibody for preventing CA 242, CEA, CA 50, CA 199are marked by Eu3+, Tb3+, Sm3+ and Dy3+. The detection kit can be used for clinical auxiliary diagnosis, curative effect observation and prognosis of the pancreatic cancer and capable of detecting for tumor markers at the same time and simplifying detecting steps and improving specificity and sensitivity of test data comprehensive analysis.

Description

technical field [0001] The invention relates to the technical field of biological and medical detection, in particular to a time-resolved fluorescent immunoassay kit for simultaneously comprehensively detecting pancreatic cancer tumor markers CA242, CA50, CEA, and CA199 in the same reaction system, and also relates to the kit Application in comprehensive detection of pancreatic cancer tumor markers CA242, CA50, CEA, CA199. Background technique [0002] Pancreatic cancer is one of the common malignant tumors of the digestive tract. Due to the lack of typical clinical manifestations in the early stage, more than 90% of the patients are already in the middle and late stage when they are diagnosed clinically, losing the best time for treatment, and their 5-year survival rate is only 5%. Among the common cancers in the world, pancreatic cancer ranks fourth in men and fifth in women, and its prognosis is poor. The current research hotspot is to explore specific tumor markers for ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/531G01N21/64
Inventor 王嘎程自卿
Owner 河南生生医疗器械有限公司
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