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Analysis method based on heterojunction composite material for detecting photoelectric chemical adaptor of carcino-embryonic antigen

A composite material, carcinoembryonic antigen technology, applied in material excitation analysis, analysis material, measurement device and other directions, can solve the problems of long detection time and high detection cost, and achieve low cost, good selectivity, simple structure and preparation process. Effect

Inactive Publication Date: 2018-11-23
FUZHOU UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, these methods require certain instruments and equipment, and its preparation process requires high detection costs due to the need for enzymes, and the detection time is long, which is suitable for the detection of a large number of specimens in well-equipped laboratories

Method used

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  • Analysis method based on heterojunction composite material for detecting photoelectric chemical adaptor of carcino-embryonic antigen
  • Analysis method based on heterojunction composite material for detecting photoelectric chemical adaptor of carcino-embryonic antigen
  • Analysis method based on heterojunction composite material for detecting photoelectric chemical adaptor of carcino-embryonic antigen

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Embodiment 1

[0025] 1. g-C 3 N 4 preparation of

[0026] Weigh 2.0 g of dicyandiamide and place it in a crucible, and heat it to 550 °C for 4 h in a muffle furnace at 1.0 °C / min. After the reaction is over, the crucible is naturally cooled to room temperature, and ground to powder for use.

[0027] 2. g-C 3 N 4 / Bi 2 MoO 6 Preparation of complex

[0028] Weigh 0.5 g g-C 3 N 4 Dispersed to contain 2 mmol Bi(NO 3 ) 5 ·5H 2 O in 25 mL of distilled water. Under vigorous stirring, 0.121g Na 2 MoO 4 2H 2 O was added to the above solution, and then the resulting solution was transferred to 50 mL of polytetrafluoroethylene and heated to 160 °C for hydrothermal reaction for 12 hours. After cooling to room temperature naturally, the yellow solid product was collected by centrifugation, washed three times with water and ethanol, and dried. available from figure 1 See g-C 3 N 4 and Bi 2 MoO 6 Well combined together, enhance the separation of electron holes.

[0029] 3. Preparati...

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Abstract

The invention discloses an analysis method based on a heterojunction composite material for detecting a photoelectric chemical adaptor of a carcino-embryonic antigen. The analysis method comprises thefollowing steps: firstly, synthesizing a g-C3N4 / Bi2MoO6 heterojunction photoelectric material, loading the g-C3N4 / Bi2MoO6 heterojunction photoelectric material on the surface of a heterojunction by capturing a gold nano modified adaptor nano probe by virtue of the interaction with pi-pi, and finally using the composite as a photoelectric sensing element for detecting CEA. The photoelectric DNA established by the invention is low in sensing cost, environmentally friendly, good in stability, simple in preparation process and high in sensitivity.

Description

technical field [0001] The invention relates to the technical field of photoelectrochemical aptamer sensing, in particular to an analysis method for detecting carcinoembryonic antigen photoelectrochemical aptamers based on heterojunction composite materials. Specifically g-C 3 N 4 / Bi 2 MoO 6 The heterojunction optoelectronic material is used as the base material, and the gold nano-modified CEA aptamer nanoprobe is captured by π-π interaction, which is used as a photoelectrochemical aptamer analysis method for detecting carcinoembryonic antigen. Background technique [0002] Carcinoembryonic antigen CEA is a tumor-associated antigen. Colon cancer, gastric cancer, lung cancer, and bile duct cancer are common when CEA is significantly elevated; it is also elevated in liver cancer, breast cancer, ovarian cancer, and pancreatic cancer. CEA detection is of great value in monitoring patients with persistently elevated blood circulation CEA after treatment, which can indicate l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/63G01N33/574
CPCG01N21/63G01N33/574
Inventor 唐点平邱桢丽舒健张康耀吕姝臻
Owner FUZHOU UNIV
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