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Preparation method of nylon-shell glycan compound film for affinity microfilter

A chitosan, composite membrane technology, applied in chemical instruments and methods, membrane technology, semi-permeable membrane separation, etc., can solve the problems of the number of active groups on the membrane need to be further increased, the non-specific adsorption is low, etc. Mechanical properties and chemical stability, low non-specific adsorption, good hydrophilic properties

Inactive Publication Date: 2003-04-30
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned composite membrane has good hydrophilicity and low non-specific adsorption, but the number of active groups on the membrane needs to be further improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Embodiment one: 1. the activation of nylon membrane

[0010] Add 5 ml of epichlorohydrin to 10 ml of 1M NaOH solution. Put 3 pieces of nylon membranes with a pore size of 0.45 μm and a diameter of 47 mm into the solution, shake and react for 20 hours at 70° C., and wash with distilled water for 30 minutes at room temperature. 2. Coupling Chitosan

[0011] Get 1 piece of activated membrane prepared in step 1, place in 2% chitosan acetic acid solution, the concentration of acetic acid solution is 10% (volume). The reaction was shaken for 5 hours at 40°C. The membrane was taken out and placed on a vacuum filter, the solution on the surface of the membrane was sucked dry, and dried in an oven at 100° C. for 1 hour. 3. Membrane Cleaning and Preservation

[0012] Take the composite membrane prepared in step 2, and wash it successively with 1% (volume) acetic acid solution for 1 hour and distilled water for 24 hours. The film was taken out and dried in an oven at 80° C. f...

Embodiment 2

[0013] Embodiment two: 1. the activation of nylon membrane

[0014] Take 1 piece of nylon membrane with a pore size of 2 μm and a diameter of 47 mm, put it in 10 ml of 1.5% carbonyldiimidazole in acetone solution, shake it for 1 hour at room temperature, wash it with acetone for 3 times, and place it in a 60°C oven Dry in medium for 30 minutes. 2. Coupling Chitosan

[0015] The activated membrane prepared in step 1 is placed in 2% chitosan acetic acid-sodium acetate solution, and the pH value of the acetic acid-sodium acetate buffer solution is 5.4. The reaction was shaken for 5 hours at room temperature. The membrane was taken out and placed on a vacuum filter, the solution on the surface of the membrane was blotted dry, and dried in an oven at 60° C. for 45 minutes.

[0016] Clean and preserve according to the method of Example 1 to obtain a nylon-chitosan composite membrane.

Embodiment 3

[0017] Embodiment three: 1. the activation of nylon membrane

[0018] Take 3 pieces of nylon membranes with a pore size of 1 μm and a diameter of 47 mm, put them in 10 ml of 36.5% formaldehyde solution, add 0.1 ml of 85% phosphoric acid dropwise, shake and react for 7 hours at 60°C, and wash with distilled water at 50°C 45 minutes. 2. Coupling Chitosan

[0019] Place the activated membrane prepared in step 1 in 2% chitosan acetic acid solution of pH 2, the concentration of acetic acid solution is 1% (volume), at 30° C., shake and react for 30 minutes. The membrane was taken out and placed on a vacuum filter, the solution on the surface of the membrane was sucked dry, and dried in an oven at 80° C. for 30 minutes.

[0020] Clean and preserve according to the method of Example 1 to obtain a nylon-chitosan composite membrane.

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Abstract

A process for preparing the nylon-chitosan membrane used for affinity microfilter includes such steps as activating the nylon membrane with 0.4-3 microns of pore diameter by the solution of formaldehyde, biepoxyane, etc at 20-90 deg.C, coupling reaction acidic chitosan solution at 20-50 deg.C to obtain nylon-chitosan membrane, and then washing. The said membrane features high mechanical performance, chemical stability, hydrophilicity and biocompatibility, low non-specific adsorpativity, and rich active groups.

Description

technical field [0001] The invention relates to a preparation method of a nylon-chitosan composite membrane for affinity microfiltration, which belongs to the preparation technology of the microfiltration composite membrane. Background technique [0002] Affinity microfiltration is mainly based on the biospecific interaction between the substance to be separated and the affinity ligand bonded to the membrane to achieve the separation of the target substance. The filter membrane used in affinity microfiltration should have good chemical stability, excellent chemical reaction performance, and not affect the activity of biological substances. Modified nylon membrane is currently an ideal filter membrane for affinity microfiltration. Beeskow et al. covalently bonded hydroxyethyl cellulose to the surface and pore walls of nylon membranes, and introduced active -OH groups into the composite membranes, which were 3-5 times higher than the number of acti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D69/12B01D71/08
Inventor 石巍张凤宝张国亮王淑兰赵永江
Owner TIANJIN UNIV
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