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Phenylboronic-acid-functionalized graphene oxide composite nano material and preparation and application thereof

A graphene composite, nanomaterial technology, applied in the directions of alkali metal compounds, chemical instruments and methods, alkali metal oxides/hydroxides, etc., can solve the problem of low glycoprotein selectivity, low phenylboronic acid monomer affinity, binding Weak ability and other problems, to achieve the effects of environmental friendliness, simple preparation and mild conditions

Active Publication Date: 2017-03-08
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional phenylboronic acid monomer (PBA) has low affinity and weak binding ability to cis-o-diol, resulting in low selectivity to glycoproteins.

Method used

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  • Phenylboronic-acid-functionalized graphene oxide composite nano material and preparation and application thereof
  • Phenylboronic-acid-functionalized graphene oxide composite nano material and preparation and application thereof
  • Phenylboronic-acid-functionalized graphene oxide composite nano material and preparation and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0028] 1. Fe 3 o 4 Preparation of / PDA / GO / BPEI / CBX: (1) Weigh 1.08g of FeCl 3 ·6H 2 O and 0.2g of trisodium citrate, add 20ml of ethylene glycol, and ultrasonically disperse evenly. Add 1.2 g of anhydrous sodium acetate, and stir magnetically for at least 30 min. Finally, it was transferred to the reactor and reacted at 200°C for 10h. After the reaction was completed, the product was washed three times with deionized water and absolute ethanol, and vacuum-dried to obtain Fe 3 o 4 . (2) Take 100mg of Fe 3 o 4 , add 50ml of 10mM Tris-HCl buffered saline solution with pH=8.5, and disperse evenly by ultrasonic. Quickly add 100 mg of dopamine hydrochloride (DA), and bathe in water at 25°C for 2 hours to obtain Fe 3 o 4 / PDA. After the reaction was completed, the product was washed three times each with deionized water and absolute ethanol, and dried in vacuum. (3) Prepare 1mg / ml GO aqueous solution, and evenly dispersed Fe 3 o 4 / PDA aqueous solution, the mass ratio ...

Embodiment 2

[0031] 1. Fe 3 o 4 Preparation of / PDOPA / GO / PAMAM / CBX: (1) Weigh 1.08g of FeCl 3 ·6H 2O and 0.2g of trisodium citrate, add 20ml of ethylene glycol, and ultrasonically disperse evenly. Add 1.2 g of anhydrous sodium acetate, and stir magnetically for at least 30 min. Finally, it was transferred to the reactor and reacted at 200°C for 10h. After the reaction was completed, the product was washed three times with deionized water and absolute ethanol, and vacuum-dried to obtain Fe 3 o 4 . (2) Take 100mg of Fe 3 o 4 , add 50ml of 10mM Tris-HCl buffered saline solution with pH=8.5, and disperse evenly by ultrasonic. Quickly add 100 mg of 3,4-dihydroxyphenylalanine (DOPA), and bathe in water at 25°C for 2 hours to obtain Fe 3 o 4 / PDOPA. After the reaction was completed, the product was washed three times each with deionized water and absolute ethanol, and dried in vacuum. (3) Prepare 1mg / ml GO aqueous solution, and evenly dispersed Fe 3 o 4 / PDOPA aqueous solution, the...

Embodiment 3

[0035] 1. Fe 3 o 4 Preparation of / PDA / GO / BPEI / CPBA: (1) Weigh 1.08g of FeCl 3 ·6H 2 O and 0.2g of trisodium citrate, add 20ml of ethylene glycol, and ultrasonically disperse evenly. Add 1.2 g of anhydrous sodium acetate, and stir magnetically for at least 30 min. Finally, it was transferred to the reactor and reacted at 200°C for 10h. After the reaction was completed, the product was washed three times with deionized water and absolute ethanol, and vacuum-dried to obtain Fe 3 o 4 . (2) Take 100mg of Fe 3 o 4 , add 50ml of 10mM Tris-HCl buffered saline solution with pH=8.5, and disperse evenly by ultrasonic. Quickly add 100 mg of dopamine hydrochloride and bathe in water at 25°C for 2 hours to obtain Fe 3 o 4 / PDA. After the reaction was completed, the product was washed three times each with deionized water and absolute ethanol, and dried in vacuum. (3) Prepare 1mg / ml GO aqueous solution, and evenly dispersed Fe 3 o 4 / PDA aqueous solution, the mass ratio of th...

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Abstract

The present invention relates to a new phenylboronic-acid-functionalized graphene oxide composite nano material and preparation and application thereof. The material is prepared by immobilization of polydopamine-packed magnetic nanoparticles onto polyethyleneimine-modified graphene oxide and further introduction of phenylboronic acid monomers with carboxyl by amino of polyethyleneimine, and finally the material is used for enrichment of glycoproteins. The specific process is as follows: firstly, Fe3O4 magnetic nanoparticles are prepared by a solvothermal method, and auto polymerization of polydopamine can be performed on surface of the Fe3O4 magnetic nanoparticles under alkaline conditions; the magnetic graphene oxide composite nano material can be prepared by hydrogen bond and PI-PI interaction between the polydopamine and graphene oxide, then the positively charged polyethyleneimine is immobilized onto the surface of the negatively charged magnetic graphene oxide by electrostatic self-assembly; and finally the phenylboronic-acid-functionalized magnetic graphene oxide composite nano material can be prepared by introduction of the phenylboronic acid monomers by amidation reaction, and the phenylboronic-acid-functionalized magnetic graphene oxide composite nano material is successively used in the enrichment of the glycoproteins in bioanalysis.

Description

technical field [0001] The invention relates to a graphene oxide composite nanometer material functionalized with phenylboronic acid and its preparation and application to the specific enrichment of glycoproteins. Background technique [0002] The boron affinity strategy has attracted more and more attention in the separation and enrichment of glycosylated proteins because of its simple operation, unbiasedness, and non-destructive glycoform structure. However, the traditional phenylboronic acid monomer (PBA) has low affinity and weak binding ability to cis-o-diols, resulting in low selectivity to glycoproteins. According to literature reports, a new type of phenylboronic acid (2-hydroxymethylphenylboronic acid cyclic monoester and its derivatives) has a low pKa value (pKa=7.3), and its binding ability with cis-o-diol is higher than that of traditional phenylboronic acid Higher, so it is a very potential new monomer, which has been successfully applied to the enrichment of g...

Claims

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Application Information

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IPC IPC(8): B01J20/26B01J20/28B01J20/30
Inventor 张丽华吴琼江波翁叶靖张玉奎
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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