Photoluminescent nano particle as well as preparation method and application thereof

Abstract

The invention discloses a photoluminescent nano particle as well as a preparation method and an application thereof. The photoluminescent nano particle is composed of a carboxyl-containing copolymer matrix material and a rare earth fluorescent dye dispersed in the matrix material. The preparation method of the fluorescent nano particle comprises the following steps: dissolving the rare earth compound fluorescent dye and the copolymer in an organic solvent which is capable of being miscible with water; and adding the solution in water, so that the fluorescent nano particle is formed by utilizing a coprecipitation-self assembly process. The fluorescent nano particle has the advantages of excellent long wave excitation luminescence property and good stability, and has a surface carboxyl group for coupling biomolecules. A biological probe based on the fluorescent nano particle has a wide application prospect in the aspects of high-sensitivity fluorescent immunoassay, biological imaging and the like.

Description

technical field [0001] The present invention relates to a class of photoinduced nanoparticles and a class of rare earth complex fluorescent dyes that can be used to prepare such nanoparticles and their preparation methods and applications, especially a class of fluorescent nanoparticles based on rare earth complex fluorescent dyes and their preparation Methods and applications. Background technique [0002] Fluorescent probes of rare earth complexes have important applications in the fields of biological imaging, DNA detection, and immunoassay. Its main advantage is that rare earth complexes have the characteristics of longer luminescence lifetime, larger Stokes shift, and sharp line emission peaks. These characteristics make it possible to effectively filter background signal interference through time-resolved technology in biochemical analysis, achieve high-sensitivity detection, and avoid the biosafety problems caused by traditional radiolabeling methods. [0003] Since...

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Problems solved by technology

However, nanoparticles based on common hydrophobic polymers are prone to agglomeration in aqueous solution, and their particle size is large, and the scattering signal is strong.

The silica matrix also often leads to the leakage of fluorescent dyes in the formed nanoparticles due to the difficulty in forming a dense encapsulation layer

[0007] The inventors of the present invention have used the reported rare earth complex-based fluorescent nanoparticle synthesis method (such as microemulsion polymerization, alkoxysilane hydrolysis embedding method, etc.), to design and synthesize Eu(tta) 3 Rare earth complexes with excellent visible and near-infrared light-excited luminescent properties such as dpbt are nano-embedded to synthesize new rare earth complex-based fluorescent nanoparticles, but it is difficult to obtain satisfactory results.

The main reason is that the coordination between ligands and rare earth ions of such rare earth complexes with excellent visible light and near-infrared excitation and luminescence properties is weak, and the usual complex embedding methods easily lead to ligand dissociation or fluorescence quenching. Destroyed, lost the original optical properties

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