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Antigen-immobilized matrix membrane, kit comprising the same for detecting antinuclear antibody spectrum related to autoimmune diseases and purpose thereof

An autoimmune disease and antigen detection technology, applied in the field of biomedicine, can solve the problems of incomplete disease types, complex operations, low specificity and sensitivity, etc., and achieve intuitive and accurate result interpretation, high detection efficiency and high specificity Effect

Pending Publication Date: 2019-10-18
英诺诊断有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] The technical problem to be solved by the present invention is to provide an antigen-immobilized matrix membrane and The kit containing it for detecting the spectrum of antinuclear antibodies associated with autoimmune diseases and its application, the kit containing the antigen-immobilized matrix membrane can simultaneously detect 22 kinds of autoimmune antibodies, and is used to assist in the diagnosis of 8 common diffuse Connective tissue disease, and has extremely high specificity and sensitivity, the kit of the present invention including the reaction membrane strip that can detect at least 22 kinds of autoimmune antibodies is the first case at home and abroad

Method used

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  • Antigen-immobilized matrix membrane, kit comprising the same for detecting antinuclear antibody spectrum related to autoimmune diseases and purpose thereof
  • Antigen-immobilized matrix membrane, kit comprising the same for detecting antinuclear antibody spectrum related to autoimmune diseases and purpose thereof
  • Antigen-immobilized matrix membrane, kit comprising the same for detecting antinuclear antibody spectrum related to autoimmune diseases and purpose thereof

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Effect test

Embodiment 1

[0044] The preparation of embodiment 1 kit

[0045] 1. Antigen preparation

[0046] All autoantigen proteins were expressed in recombinant protein using mammalian cell expression system.

[0047] Entrust the biological company to carry out the sequence of different antigens (Jo-1: Gene ID 3035; PL-7: Gene ID 6897; PL-12: Gene ID 16; SnRNP-A: Gene ID 6627; SnRNP-C: Gene ID 6631; SnRNP68 / 70: Gene ID6625; SmD1: Gene ID 6632; SmD3: Gene ID 6634; SmB / B': Gene ID 6628; dsDNA: PMID: 6272877; H2A: Gene ID 92815; H2B: Gene ID 8349; H3: Gene ID 8350; H4: Gene ID 8359; PCNA: Gene ID 5111; P0: Gene ID 6175; SSA / Ro60: Gene ID 6738; SSA / Ro52: Gene ID 6737; SSB / La: Gene ID 6741; CENP-A: Gene ID 1058; CENP -B: Gene ID 1059; Scl-70: Gene ID 7150; PmScl-75: Gene ID 5393; RA33: Gene ID 3181; PR3: Gene ID 5657) were synthesized and the resulting sequence was cloned into the pcDNA3.1 (+) expression vector by Ampicillin (final concentration 0.1mg / ml) was used to screen the resistant positive cl...

Embodiment 2

[0066] The usage method of embodiment 2 kit

[0067] Sample preparation: human serum or anticoagulated plasma. The samples are stored at 2-4°C. If the test cannot be performed within 24 hours, the samples should be stored at -20 or -80°C. Specimens with severe hemolysis, floc or mold will affect the test results and should not be used.

[0068] Washing solution preparation: dilute 1 part of 20× concentrated washing solution with 19 parts of distilled water, mix well to prepare a standard washing solution (need to be prepared and used immediately).

[0069] (1) Put the reaction membrane strip into a semi-closed independent reaction groove in the reaction incubation plate, add 1ml of standard washing solution, shake gently until the membrane strip is completely wet, then discard the dilution solution.

[0070] (2) Dilute the sample to be tested with the sample diluent at a ratio of 1 (sample serum): 250 (diluent), and add 1ml of the diluted sample to the membrane strip reactio...

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Abstract

The invention discloses an antigen-immobilized matrix membrane, a kit comprising the same for detecting an antinuclear antibody spectrum related to autoimmune diseases and purpose thereof. The antigen-immobilized matrix membrane comprises but not only comprises following 22 mutually independent antigen detection lines including double-stranded DNA, histone, ribosome P0 protein, PCNA, SmD1, SmD3, SmB / B', snRNP-A, snRNP-C, snRNP68 / 70, SSA / Ro60, SSA / Ro52, SSB / La, Jo-1, PL-7, PL-12, PmScl, Scl-70, CENP-A, CENP-B, RA33 and PR3. When the kit containing the antigen-immobilized matrix membrane is usedfor detection, 22 kinds of autoimmune antibodies can be detected simultaneously; and thus diagnosis of eight kinds of common diffuse connective tissue diseases is assisted.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to an antigen-immobilized matrix membrane and a kit containing it for detecting antinuclear antibody spectrum related to autoimmune diseases and its application. Background technique [0002] Autoimmune diseases are a class of diseases in which the body's immune system reacts to its own antigens, resulting in damage to its own organs and tissues. Its pathogenesis involves the exposure of self-antigens, the pro-inflammatory microenvironment of the body, the activation of the innate immune system, the activation of the acquired immune system by antigen presentation, the activation of T cells, and the release of a large number of anti-autoantibodies by B cells. With the hierarchical disorder of the immune system and further attack and damage to the tissues or organs that release self-antigens, organic lesions, that is, autoimmune diseases, eventually occur. [0003] Based on the...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/535
CPCG01N33/6893G01N33/535G01N2800/24
Inventor 徐爱民钟铃韩灏凯·基恩周鹏程左陪军
Owner 英诺诊断有限公司
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