Digital microfluidic autoimmune antibody multiple detection system

An autoimmune antibody and multiple detection technology, applied in the field of digital microfluidics, can solve the problems of difficulty in meeting the needs of autoimmune detection, high cost of immune analyzers, and limited use scenarios, achieving low cost, low equipment cost, and high detection flux effect

Inactive Publication Date: 2021-07-30
JIANGSU LOGILET BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Thermo Fisher Phadia TM The 250 immunoassay analyzer is expensive, bulky and has limited usage scenarios, making it difficult to meet the huge demand for autoimmune testing

Method used

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  • Digital microfluidic autoimmune antibody multiple detection system
  • Digital microfluidic autoimmune antibody multiple detection system
  • Digital microfluidic autoimmune antibody multiple detection system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1: Autoimmune detection of 8 antigens and 1 secondary antibody in 1 sample

[0045] (1) Load the chip on the detection equipment and fill the chip with biocompatible medium oil, according to image 3 Load samples and reagents according to the specified volume at the corresponding inlet (in this embodiment, a single reaction requires 20 microliters of samples, so at least 160 microliters of samples need to be injected into the chip);

[0046] (2) if Figure 4 As shown, the sample 10 is quantitatively divided into 8 sample droplets 13 according to the direction of the arrow in the figure and transferred to the positions of the 8 immobilized antigens respectively, and the incubation reaction is carried out for 3 minutes. The reaction temperature is usually 37 degrees Celsius. After the incubation reaction is completed, the sample The droplet 13 is transferred to the waste liquid area 9 and discarded;

[0047] (3) if Figure 5 As shown, the cleaning solution 11 is ...

Embodiment 2

[0051] Example 2: Autoimmune detection of 16 antigens and 2 secondary antibodies in 1 sample

[0052] Figure 9 It shows the design of the digital microfluidic autoimmune antibody detection chip that can carry out two kinds of secondary antibodies at the same time, including multiple immobilized antigens on the lower surface of the upper cover 2, reagent and sample distribution area 8, waste liquid area 9, sample solution 10, Cleaning solution 11 , the first fluorescently labeled secondary antibody solution 12 , and the second fluorescently labeled secondary antibody solution 16 .

[0053] (1) Load the chip on the detection equipment and fill the chip with biocompatible medium oil, according to Figure 9 Load samples and reagents according to the specified volume at the corresponding inlet (in this embodiment, a single reaction requires 20 microliters of samples, so at least 320 microliters of samples need to be injected into the chip);

[0054] (2) Figure 9 Contains two c...

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Abstract

The invention provides a digital microfluidic autoimmune antibody detection system. According to the method, the whole-process autoimmune antibody detection of a sample is carried out on a single chip, all operations related in an experiment process, including sample quantitative distribution, reagent quantitative distribution, solution transfer, incubation reaction, antigen cleaning and the like, are automatically completed by a digital micro-fluidic chip, and finally, a detection result is judged through fluorescence detection. A user only needs to load a sample to the chip, and the whole-process multiple autoimmune antibody detection can be realized.

Description

technical field [0001] The invention belongs to the technical field of digital microfluidics, and relates to a digital microfluidic multiple detection system for autoimmune antibodies. Background technique [0002] Autoimmune antibody detection is one of the important detection methods for autoimmune diseases. At present, the domestic autoimmune detection technology is still in its infancy, and the detection process mainly relies on manual operations or large workstations based on robotic arms. At present, the domestic auto-immune testing market is mainly occupied by international companies, such as Thermo Fisher Phadia TM The 250 Immunoanalyzer is a robotic arm-based workstation for medium-sized laboratories that can deliver 400 to 2000 test results per week. [0003] Since the self-exemption detection process is relatively complicated and time-consuming and the detection efficiency is low, it is easy to fail the self-exemption detection due to misoperation in the manual o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00G01N33/53G01N33/564G01N33/58
CPCB01L3/5027B01L3/502784G01N33/564G01N33/582G01N33/5304
Inventor 苏阳张研
Owner JIANGSU LOGILET BIOTECH CO LTD
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