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Application of KRT84 self-immune antibody as diagnosis marker for diagnosing and detecting rheumatoid arthritis

A KRT84, 2. KRT84 technology is applied in the application field of KRT84 autoimmune antibody as a diagnostic marker for diagnosing and detecting rheumatoid arthritis, which can solve problems such as not being systematically studied and screened

Inactive Publication Date: 2014-06-25
常晓天 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although citrullinated proteins like filaggrin, fibrin, and vimentin have been identified as citrullinated protein antigens for RA autoimmune antibodies, so far, citrullinated proteins as RA autoimmune antigens have not been identified. Systematic research, screening

Method used

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  • Application of KRT84 self-immune antibody as diagnosis marker for diagnosing and detecting rheumatoid arthritis
  • Application of KRT84 self-immune antibody as diagnosis marker for diagnosing and detecting rheumatoid arthritis
  • Application of KRT84 self-immune antibody as diagnosis marker for diagnosing and detecting rheumatoid arthritis

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Embodiment Construction

[0010] Below in conjunction with experiment the present invention will be further described.

[0011] experiment

[0012] 1. Design principle

[0013] The histopathological features of RA are synovial inflammation and hyperplasia, and the secretion of a large number of autoimmune antibodies by B cell clusters under the stimulation of autoimmune antigens. We first separated the total protein of RA synovium by two-dimensional gel electrophoresis, and then transferred the protein to polyvinylidene fluoride (PVDF) membrane, and then used purified RA patient serum and anti-citrulline antibody as probes, respectively. needle hybridization. Proteins recognized by both citrullinated antibodies and in the blood of RA patients are considered RA citrullinated autoimmune antigens. Finally, matrix-assisted laser desorption ionization time-of-flight mass spectrometry was used to analyze and identify RA citrullinated autoimmune antigens to determine which proteins these proteins were. Fo...

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Abstract

The invention discloses application of a KRT84 self-immune antibody as a diagnosis marker for diagnosing and detecting rheumatoid arthritis (RA). According to the application disclosed by the invention, the reality that the expression level of the KRT84-resistant self-immune antibody in the blood of an RA patient is remarkably improved compared with that of a normal person through experimental research (a two-dimensional western blotting method, a proteomic technology and an ELISA (Enzyme-Linked Immuno Sorbent Assay) technology), so that the KRT84-resistant self-immune antibody can be used as the diagnosis marker of RA. When the KRT84 self-immune antibody is applied, immunochemical methods combining an ELISA technology, a colloidal gold test paper and general antigen and antibody specificity can be respectively used for detecting the KRT84 self-immune antibody.

Description

technical field [0001] The invention relates to the application of KRT84 autoimmune antibody as a diagnostic marker for diagnosing and detecting rheumatoid arthritis. Background technique [0002] Rheumatoid arthritis (rheumatoidarthritis, RA) is an autoimmune disease. Clinically, the method of detecting the existence and expression level of autoimmune antibodies provides a basis for the diagnosis of RA. Autoimmune antibodies commonly used in the detection of RA include rheumatoid factor (RF), anti filaggrin autoantibody (AFA), anti keratin antibody (AKA), anti-nuclear perinuclear factor ( anti perinuclear factor (APF), anti vimentin (anti vimentin) and anti cyclic citrullinated peptide antibody (anti cyclic citrullinated peptide antibody, anti-CCP). However, the intellectual property rights of the above RA diagnostic markers all belong to western developed countries. These diagnostic reagents are expensive in the Chinese medical market, and the price of a test kit for 96 ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68C07K14/47
Inventor 常晓天孟红
Owner 常晓天
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