Kit and method for detecting autoimmune antibody of type-I diabetes mellitus

Abstract

The invention provides a kit and method for detecting an autoimmune antibody of the type-I diabetes mellitus and belongs to the technical field of biochemical medicine detection. The method comprises the following steps of: adding a luciferase as an antibody fusion protein, wherein the luciferase as the antibody fusion protein is generated by 293 cell culture and can be specifically combined with a diabetes mellitus autoantibody in serum of a patient; then, adding protein-A agarose, depositing a fusion protein as an antibody compound, centrifuging, and then, absorbing the uncombined fusion protein from a supernatant liquid; and then, adding a luciferase substrate, and detecting the fluorescence intensity by using a fluorescence detection instrument to finally measure the content of the diabetes mellitus autoantibody in a sample to be detected. Compared with the traditional HPLC (High Performance Liquid Chromatography), a micro-quantitative fluorescence detection instrument used for detecting in the method has the advantages of simplicity in operation, no need of uncovering to block a pollution way, high sensitivity and signal to noise ratio, stable and reliable measured value and capability of ensuring reliable experiment result and safety of operating personnel and meeting the requirements of micro quantity and regent saving.

Description

[0001] technical field [0002] The invention relates to the technical field of biochemical medical testing, in particular to a test kit for detecting type I diabetes autoimmune antibodies and a detection method thereof, which can be used for the prediction and diagnosis of type I diabetes. Background technique [0003] Generally, type I diabetes is an autoimmune disease. The body is induced by infection (especially viral infection), poison and other factors to produce abnormal self-humoral and cellular immune responses, resulting in damage to islet β cells and decreased insulin secretion. Rely on exogenous insulin supplement to maintain life, so type Ⅰ diabetes is also called insulin-dependent diabetes. [0004] This form of diabetes is common in children and young adults, but it can occur in people of any age and accounts for approximately 10% of all diabetes cases. It can be seen that early diagnosis of type Ⅰ diabetes and prediction in high-risk groups are of great sign...

AI Technical Summary

Problems solved by technology

This method sometimes has cross-reactions, false-positive reactions, tissue sample processing is not fast enough, cannot inactivate degradative enzymes and salts, and sometimes affects the results, etc. There are also problems such as radiation and pollution caused by the use of radioactive markers