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Microfluidic biological logic gate for ocean copper ion detection

A copper ion detection and logic gate technology, applied in the field of biological logic gates, can solve the problems of readout relying on large-scale instruments, low output, high cost, etc., and achieves the effect of convenient operation and easy acquisition.

Active Publication Date: 2022-04-08
YANGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the existing fluorescence methods need to prepare ligands or quantum dots for electronic conversion with copper ions, or develop a material for electronic conversion with copper ions, and most of the existing colorimetric methods need to develop new reagents or ligands for display. color reaction
The preparation process of detection reagents in this type of technology is extremely complicated, the cost is high and the yield is not high, the fluorescence signal is easily quenched, and the readout depends on large instruments, which limits the practical application of this type of technology to a certain extent

Method used

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  • Microfluidic biological logic gate for ocean copper ion detection
  • Microfluidic biological logic gate for ocean copper ion detection
  • Microfluidic biological logic gate for ocean copper ion detection

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Embodiment 1

[0050] refer to Figure 1 ~ Figure 3 , which is the first embodiment of the present invention, which provides a microfluidic biological logic gate for marine copper ion detection, which realizes the rapid detection of sea water quality through the setting of logic structure, in the field of biological computers It has potential application value, and at the same time, the logical structure and the functional chip body are integrated to complement each other, and realize the rapid identification of whether copper ions exist in seawater.

[0051] A microfluidic biological logic gate for detection of marine copper ions, comprising,

[0052] A monovalent copper ion generation unit, the monovalent copper ion generation unit is used to react the reagent to be detected and the reaction solution in the first logic gate reaction channel 107, and whether to generate monovalent copper ions and the concentration of monovalent copper ions as the first an output signal of a logic gate;

...

Embodiment 2

[0067] For the second embodiment of the present invention, the difference from the first embodiment is that this embodiment provides a microfluidic biological logic gate for marine copper ion detection, which can realize seawater copper divalent Detection of whether the ion exceeds the standard.

[0068] Define that the reaction solution is added to the reaction solution storage pool 108, the input signal is 1, and the input signal without the reaction solution is 0;

[0069] Define that the active agent is added to the active agent storage pool 110, the input signal is 1, and the input signal without the active agent is 0;

[0070] Define that the color developing solution is added into the first control solution storage pool 113, the input signal is 1, and the input signal without the color developing solution is 0;

[0071] The color of the solution in the signal output storage pool 112 is lighter than the color of the solution in the signal output storage pool 112 when de...

Embodiment 3

[0073] For the third embodiment of the present invention, the difference from Embodiment 1 and Embodiment 2 is that this embodiment provides a microfluidic biological logic gate for ocean copper ion detection, which can realize copper ion precise detection.

[0074] Wherein, the reagent to be detected, the reaction solution added in the reaction solution storage tank 108, the active agent added in the active agent storage tank 110, the first control solution added in the first control solution storage tank 113, the second control solution storage tank 114 The volume ratio of the second control solution added internally is 1:1:18:20:10.

[0075] Further, add 100 μM sodium ascorbate to the reaction solution storage through the reaction solution injection hole 104, add 12.5 ng / mL active agent to the active agent storage pool 110 through the active agent injection hole 102, and add 12.5 ng / mL active agent through the second control solution injection hole. 106 Add 2M hydrochloric...

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Abstract

The invention discloses a microfluidic biological logic gate for ocean copper ion detection, which comprises a cuprous ion generation unit, and the cuprous ion generation unit is used as an output signal of a first logic gate according to the concentration of cuprous ions; an inhibitory activity generating unit which takes whether the activity of the active agent is inhibited as an output signal of the second logic gate; the color development control generation unit is used for controlling output of a color development solution, and whether a control solution for controlling reaction starting or reaction stopping is output or not serves as an output signal of a third logic gate; the total output unit is used for taking whether the color developing solution is output or the color developing depth after being compared with the color developing condition of the standard sample as a total output signal; the arithmetic unit is used for judging the output signal of the first logic gate according to the total output signal and the output signal of the second logic gate so as to detect whether the concentration of the bivalent copper ions in the seawater exceeds the standard or not; the method is convenient to detect and low in cost.

Description

technical field [0001] The invention relates to a logic device, in particular to a biological logic gate for detecting marine copper ions. Background technique [0002] Marine environmental pollution is an increasingly serious global problem, among which heavy metal ions exceeding the standard have caused lasting damage to the marine environment. Trace copper ions are an indispensable nutrient in the ocean, and its concentration changes with factors such as seawater salinity, temperature, depth, and pH. The concentration of copper ions in normal seawater is in fM-pM, however, the concentration of copper ions in polluted seawater can be as high as μM. High concentrations of copper ions have serious effects on the metabolism of organisms, such as affecting the fertilization of sea urchins and inhibiting the growth of algae. For humans, high concentrations of copper ions can induce diseases such as Alzheimer's disease. China's seawater quality standards stipulate that the co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N33/18B01L3/00
Inventor 尹彬沣万心华钱长成岳文凯
Owner YANGZHOU UNIV
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