Autoimmunity antibody detection kit and detection method

Abstract

The invention relates to an autoimmunity antibody detection kit and a detection method. The detection kit comprises an antigen-coated strip-shaped solid phase carrier, an enzyme-labeled conjugate, an enzyme substrate, a confining liquid and a buffer solution. The detection method comprises the following steps that: a sample to be tested serum or plasma and the antigen-coated strip-shaped solid phase carrier are incubated at room temperature; after incubation, the solid phase carrier is washed with the buffer solution; the enzyme-labeled conjugate and the solid phase carrier are incubated at room temperature; after incubation, the solid phase carrier is washed with the buffer solution; the solid phase carrier and the enzyme substrate are incubated at room temperature; after incubation, the solid phase carrier is washed with deionized water and dried; and the dried solid phase carrier is put into a detection system. The detection method provided by the invention is simple, rapid and sensitive to operate, and the detection kit and the detection method have good specificity and low requirements for operators; and the interpretation of detection results are basically unaffected by the professional knowledge and experience of testers and other factors, therefore, the detection kit and the detection method can be widely applied to all levels of medical inspection places, especially primary-level medical and health care institutions.

Description

technical field [0001] The invention relates to an autoimmune antibody detection kit and a detection method, which belong to the field of medical examination. Background technique [0002] Autoimmune disease (AID) refers to the reduction or destruction of the immune system's immune tolerance to its own components due to some reasons (infection, genetics, endocrine, etc.), resulting in autoantibodies and / or sensitized lymphocytes and some Diseases caused by innate immune components such as complement, inflammatory factors, etc., damage their own organs and tissues, which manifest as dysfunctions of the corresponding tissues and organs. [0003] Autoimmune diseases include more than 30 diseases in 11 categories, including systemic lupus erythematosus, mixed connective tissue disease, progressive systemic scleroderma, polymyositis, autoimmune liver disease, rheumatism and rheumatoid disease, I Type diabetes and malignant tumors are the most common diseases that seriously affec...

AI Technical Summary

Problems solved by technology

[0006] At present, the clinical autoantibody detection methods mainly include indirect immunofluorescence, enzyme-linked immunosorbent assay, western blotting, immunospot method, immunoprecipitation method, etc., most of which are still mainly manual operations, and the operation process is relatively complicated. The requirements for personnel are high, and the interpretation of the test results is affected by multiple factors such as the test method, the type of test reagent, the professional knowledge and experience of the test personnel, etc.

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