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Kit for detecting autoimmune antibodies at throughput

A technology of autoimmune antibodies and kits, applied in the direction of biological testing, measuring devices, material inspection products, etc., can solve the problems of high production cost, low detection capacity, and inability to meet the needs of antibodies, etc., and achieve low production costs and excellent production methods. simple effect

Inactive Publication Date: 2011-05-11
深圳市宏信生物技术有限公司
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

Therefore, the detection capacity of this method is low, and its low detection capacity cannot meet the needs of detecting more antibodies in autoimmune diseases
Moreover, using the LIA method to print one protein at a time, the operation is complicated and the production cost is high

Method used

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  • Kit for detecting autoimmune antibodies at throughput
  • Kit for detecting autoimmune antibodies at throughput
  • Kit for detecting autoimmune antibodies at throughput

Examples

Experimental program
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Embodiment Construction

[0020] The following is a further detailed description with reference to the preferred embodiments shown in the drawings.

[0021] Examples of the kit for high-throughput detection of autoimmune antibodies of the present invention include: 1. a membrane printed with multiple antigens; 2. anti-human IgG-enzyme conjugate; 3. indicator dye, and 4. quality control substance . among them,

[0022] 1. Film printing

[0023] 1.1. The membrane is a nitrocellulose membrane with a thickness of 0.1mm and a pore size of 0.45um. The membrane can be used alone, coated on a glass substrate, or on a substrate made of other inert materials.

[0024] 1.2. The antigen to be printed is diluted with a buffer to an appropriate concentration and then added with a transient dye to monitor whether there is any omission during the printing process. The transient dye contains bromophenol blue.

[0025] 1.3. The production tool is to use capillary, pipette tip, needle and other multi-channel spotting devices to ...

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Abstract

The invention relates to a kit for detecting autoimmune antibodies at throughput, which mainly comprises a membrane, wherein a plurality of antigens are subjected to dot printing on the membrane in the self-determined order and in a physical adsorption mode of direct contact; and the membrane can adsorb a plurality of required antigens, and can detect a plurality of autoantibodies simultaneously in high throughput with other components of the kit. In addition, the kit has a simple and quick production method, and low production cost.

Description

Technical field [0001] The present invention relates to antibody detection, in particular to high-throughput detection of autoimmune antibodies. Background technique [0002] The clinical manifestations of autoimmune diseases are complex and diverse, and they are highly dependent on laboratory tests. At present, the clinically commonly used diagnostic techniques at home and abroad include indirect immunofluorescence staining, western blot analysis, enzyme-linked immunoassay, latex flow rapid detection, and Hep2 cell detection for antinuclear antibodies. The indirect immunofluorescence staining detection method is: pre-fixed and used to incubate and test the serum. The antibody-positive serum is combined with the pre-fixed antigen, and the result is stained in the nuclear or cytoplasm due to different antigens or different autoimmune diseases More similar staining may be produced, so further identification is usually required to identify autoantibodies and diagnose related autoim...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/551G01N33/577G01N33/52G01N33/96
Inventor 李先强姜昕
Owner 深圳市宏信生物技术有限公司
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