Method and reagent box for testing rabies viruses

A technology for quantitative detection of rabies virus, applied in the field of detection of rabies virus and kits, can solve the problem of high price of anti-rabies serum

Active Publication Date: 2008-05-14
DAAN GENE CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high price of rabies vaccine and anti-rabies serum, the establishment of a cheaper, sensitive and specific early diagnosis method should have broad application prospects

Method used

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  • Method and reagent box for testing rabies viruses
  • Method and reagent box for testing rabies viruses
  • Method and reagent box for testing rabies viruses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0049] (1) Prepare a kit including the following components: RNA extraction solution A (100 μl / tube), RNA extraction solution B (4ml / bottle), reverse transcriptase system (20 μl / 1 tube), DEPC H 2 O (2.0ml / tube), PCR reaction solution I (180μl / tube), negative control specimen (250μl / tube), strong positive quality control (200μl / tube), critical positive quality control (200μl / tube), positive Quantitative reference (1×10 6 copies / ml, 1×10 7 copies / ml, 1×10 8 copies / ml, 1×10 9 copies / ml, each 10μl / tube). Wherein the RNA extraction solution A is composed of ---; the RNA extraction solution B is composed of ---; the reverse transcriptase system includes ---; the PCR reaction solution I is composed of ---. In the present invention, - is used as a negative control; - - is used as a strong positive quality control; - - is used as a critical positive quality control and - - is used as a positive quantitative reference.

[0050] (2) Specimen collection, transportation and storage: U...

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Abstract

The invention relates to a method for the detection of the existence of rabies virus (RABV) in the clinical sample and a kit, in particular to the method for using a real-time quantitative fluorescence polymerase chain reaction technology for the early diagnosis of the rabies virus infection and the kit which is used.

Description

[0001] Field [0002] The invention relates to a method and kit for detecting the presence of rabies virus in clinical samples, in particular to a method for detecting rabies virus in samples by real-time quantitative fluorescent polymerase chain reaction technology for early diagnosis of rabies virus infection and the kit used. Background of the invention [0003] Rabies, also known as hydrophobia, is a zoonotic acute infectious disease of the central nervous system caused by rabies virus, and is a statutory Class A infectious disease. Rabies is more common in carnivores such as dogs, wolves, and cats. Human rabies is mainly caused by people being bitten by sick animals, or related to close contact with domestic animals. Once onset, the mortality rate is almost 100%. The clinical manifestations are unique mania, fear, fear of wind and water, salivation and spasm of pharyngeal muscles, and eventually paralysis occurs and life-threatening. [0004] Rabies virus belongs to th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/00C12Q1/68C12Q1/70
Inventor 唐青程钢张强黄立英李浩李玛陈剑平王方金
Owner DAAN GENE CO LTD
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