Full-automatic immuno-fluorescence quantitative analysis device and implementation method

Abstract

The invention discloses a full-automatic immuno-fluorescence quantitative analysis device and an implementation method, belonging to the field of quantitative immuno-fluorescence analysis and detection. The full-automatic immuno-fluorescence quantitative analysis device comprises a supporting bottom plate, a reagentstrip storage and automatic loading module, a reactiondisc module, a detection module, a sample module, a sample adding module, a washing module and a control system, wherein the reagentstrip storage and automatic loading module, the reactiondisc module, the detection module, the sample module, the sample adding module and the washing module are arranged on the supporting bottom plate in sequence; the reagentstrip storage and automatic loading module provides a reagent strip for the reactiondisc module; the sample adding module is used for adding samples in the sample module into the reactiondisc module to carry out reaction, and after reaction is finished, the samples enter the detection module to be detected. The full-automatic immuno-fluorescence quantitative analysis device and the implementation method disclosed by the invention have the advantages that the difficulty that automation is hard to implement for an in-vitro diagnosis product is solved, the artificial error is reduced, the testing accuracy is improved and the detection efficiency is improved.

Description

technical field [0001] The invention belongs to the field of quantitative fluorescence immunoassay detection, in particular to a fully automatic fluorescence immunoassay quantitative analysis method and device. Background technique [0002] POCT, point-of-care testing is a subdivision of in vitro diagnostic devices (IVD), that is, on-site sampling and immediate analysis, eliminating the complicated procedures of specimen testing in the laboratory and quickly obtaining test results. [0003] When performing fluorescent immunoassay quantitative detection, most of the diagnostic instruments currently on the market are semi-automatic detection instruments. For example, the existing fluorescent immunochromatography analyzer GP1100 of Nanjing Jidan Biotechnology Co., Ltd. is a semi-automatic detection instrument. Its machine structure is only It includes an optical path system and electronic display system for quantitative inspection. The process of adding samples and chromatograp...

AI Technical Summary

Problems solved by technology

[0003] When performing fluorescent immunoassay quantitative detection, most of the diagnostic instruments currently on the market are semi-automatic detection instruments. For example, the existing fluorescent immunochromatography analyzer GP1100 of Nanjing Jidan Biotechnology Co., Ltd. is a semi-automatic detection instrument. Its machine structure is only It includes an optical path system and electronic display system for quantitative inspection. The process of adding samples and chromatography is done manually outside the machine; in use, first of all, because the semi-automatic instrument adds samples outdoors, it requires high temperature for some The product inspection is very unfavorable; secondly, because of the need for manual sampling and sample addition steps, and then quantitative analysis by instruments, the test error caused by manual intervention is increased, the development time of immunochromatography, and the accuracy of sample addition are not good. Accurate, slow test speed, manual sample addition is easy to cause biological contamination; finally, most of the existing small detection instruments can only load one sample at a time, and then add another sample to it after the test is completed

This has caused the following disadvantages: there must be a special manual on duty, repeated operations to load samples, manual intervention to cause biological contamination, and at the same time, the inspectors cannot leave to perform other inspections; in addition, one at a time is inspected, which is inefficient and delays the diagnosis of the disease; Outdoor sampling is not suitable for product testing with high temperature requirements

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