88results about How to "The test results are credible" patented technology

The invention relates to amplification composite for detecting the microdeletion of Y-chromosome and a detection kit, belonging to the field of biotechnical detection. The amplification composite for detecting the microdeletion of Y-chromosome can be amplified to as many as 30 sites related to the microdeletion detection of the Y-chromosome through one reaction. The detection kit detects the microdeletion of the Y-chromosome through the quantitative fluorescent PCR (Polymerase Chain Reaction) method by using the amplification composite. The microdeletion abnormality of the Y-chromosome is determined according to the existence of an amplification product and the quantity of the amplification product. A large quantity of sites enables the detection result to be more convincible and can provide much more and more detailed information for determining the deletion type, and the quantitative detection of partial deletion and repetition can be realized. The detection kit is easier and more convenient to operate, only one PCR amplification and one sequencer detection reaction are needed to complete the detection of one sample, the whole process needs 4-5 h, and the operation intensity and the detection time are greatly reduced.

The invention relates to a test method and device of metal ceiling system intensity, which is especially applied under static pressure load and wind pressure dynamic load. The test device is composed of a sample car, a working system and an operation controlling system. Realize test and characterization of metal ceiling system intensity by simulating practical environment of its enlistment. This method can reflect practical environment of metal ceiling really, which guarantees more scientific and perfect test method and more credible test result. It will be extended as a professional testing criterion.

A high temperature testing machine for gears comprises a gear transmission case used for testing the fatigue life of the gears, wherein the gears to be tested are installed in the gear transmission case, the gear transmission case is connected with a temperature control system, the temperature control system comprises a heating device and a control module of a temperature monitor device, the heating device is connected with the gear transmission case through a pipeline, the heating device conveys lubricating oil into the gear transmission case through the pipeline to heat and lubricate the gears to be tested in an oil bath mode so that the working condition of gears inside an aviation speed reducer can be simulated, and the lubricating oil is heated to reach the set temperature. The temperature monitor device is installed on the heating device to monitor the temperature of the lubricating oil in real time, temperature information is fed back to the control module, and then the heating temperature can be monitored and controlled. Long-time simulation testing under the high temperature can be carried out on the gears, the testing temperature is high, working time is long, the testing environment is more approximate to the actual working condition of the aviation gears, and testing results are more effective and credible.

The invention relates to a method for detecting 1,5-anhydro sorbitol and a related diagnostic kit. In the method, a sample and adenosine diphosphate are reacted in the presence of adenosine diphosphate dependent hexokinase serving as a catalyst to form anhydroglucose-6-phosphoric acid and adenylic acid, the anhydroglucose-6-phosphoric acid and thio-oxidative NAD(nicotinamide adenine dinucleotide) are reacted in the presence of 1,5-AG(anhydro glucitol)-6-phosphate dehydrogenase(PDH) serving as a catalyst to form thio-reduced coenzymes and compounds of C6H11O8P, and simultaneously the generated C6H11O8P is reacted in the presence of AG-6-PDH and reduced NAD to form AG-6-P and oxidative coenzymes; and because of the cyclic amplification, the sensitivity of 6-anhydro sorbitol is improved. The invention also provides a detection kit for 1,5-anhydro sorbitol based on the method. The kit is convenient and easy to use, can quickly finish detection on a common ultraviolet / visible light analyzer or semi- / full-automatic biochemical analyzer, does not need special or additional instruments and is low in cost.

The invention discloses a zero intermediate frequency receiver quadrature error correction method, which can significantly improve the dynamic range and image frequency rejection ratio of a zero intermediate frequency receiver. The method is implemented according to the following technical scheme: a quadrature error detection module selects a baseband frequency and sends the baseband frequency toa correction waveform generation device so as to generate a test signal covering the whole receiving bandwidth, the quadrature error detection module collects the test signal of positive and negativefrequencies from the correction waveform generation device, extracts broadband parameter estimated values of a local oscillation error and a baseband error and introduces the values into a quadratureerror correction module through an error analysis module; and the quadrature error correction module analyzes frequency domain, amplitude and phase data and comprehensively generates a baseband errorcorrection filter and a direct weighting coefficient to correct the gain and phase relative error of a Q-channel in the whole frequency band range.

The invention discloses a chemiluminescence quantitative detection kit for a carbohydrate antigen 19-9 (CA19-9). The kit comprises a CA19-9 detection reaction plate, an enzyme conjugate, a luminescent substrate, a calibrator, a quality control material and washing concentrate, wherein the reaction plate is coated with a CA19-9 antibody. The kit can specifically and quantitatively detect the content of the CA19-9 in patient serum, determines prognosis level and identities benign and malignant pancreatic tumors. Compared with the conventional enzyme linked immunosorbent assay (ELISA) technology, the chemiluminescence immunoassay keeps high specificity of the ELISA technology, stability and reliability of a detection result, and convenience of operation, and can improve detection sensitivity simultaneously.

The invention discloses a carbohydrate antigen 125 chemiluminescent quantitative test kit, comprising a CA 125 detection reflection plate which is coated with an anti-CA125 antibody, an enzyme conjugate, a luminescent substrate, a calibrator, a quality control product and a washing concentrate. By the test kit provided by the invention, the content of patient serum CA125 can be detected exclusively and quantitatively, the ovarian cancer can be diagnosed, and the tumor size and the tumor types can be judged according to the level of CA125. Compared with the traditional ELISA(enzyme-linked immuno sorbent assay) technique, by the test kit provided by the invention, the high specificity, the stability and the reliability of the detection result, and the operational simplicity of the ELISA technique are reserved, and the sensitivity of the detection can be improved by adopting a chemiluminescent method.

The invention discloses a detection method for impedimental fine sticky substances in paper-making white water. The method comprises the following steps of: respectively detecting a sticky substance content of pretreated white water and a sticky substance content of untreated white water under a normal temperature and pressure condition by adopting a large sticky substance Pulmac MasterScreen method and an image analysis method; and carrying out subtraction on two obtained detection results to obtain a content of harmful fine sticky substances in the white water, wherein the process of pretreating the white water comprises the following steps of: adding paper-making white water to be detected into a temperature and pressure control pretreatment device with a stirring function to act the white water for 10-60min at 20-90 DEG C under a pressure of -0.08-5MPa with a rotation speed of 100-2,000rpm, and then taking out. The method can be used for detecting the content of the fine sticky substances having adverse effects on paper machine running, paper quality, and the like in the white water and effectively evaluating the harm extent of the sticky substances in the white water for providing most direct technical references for white water closed circulation, a reasonable design of a system of the white water closed circulation, and the like.

The invention provides a preparation method of soluble recombinant sinopotamon metallothionein (rcMT), and the preparation method has the advantages of simplicity and convenience in operation, low cost, high purity and good activity. The method comprises the following steps: firstly constructing a phoA-MT recombinant expression vector by taking a phoA plasmid as an expression vector; further transforming escherichia coli BL21, constructing an escherichia coli engineering strain phoA-MT / BL21, and inoculating the engineering strain into an LB (Luria-Bertani) culture medium to prepare seeds, then diluting bacteria to a low-phosphorus culture medium for induction and expression, and utilizing Ni sepharoseTM6 Fast Flow affinity chromatography for purification so as to get the recombinant sinopotamon metallothionein. An anti-sinopotamon metallothionein monoclonal antibody prepared by utilizing the recombinant sinopotamon metallothionein has high sensitivity and strong specificity and can be applied to ELISA (enzyme-linked immunosorbent assay) quantitative assay of the metallothionein.

The invention discloses a centering measurement apparatus for measuring the center distance of two intersecting holes in an intersecting hole system. The centering measurement apparatus comprises a two-hole positioning and centering device including an elastic centering expanding sleeve, a centering pull bar, a centering pressure bar, an eccentric wheel compacting mechanism and a positioning pin, a variable-pitch cam mechanism including a handle, a servo-actuated eccentric cam, a bearing positioning circular section and a cooperative column, and an auxiliary centering mechanism, wherein the two-hole positioning and centering device is inserted in a first hole system of a part to be measured, the eccentric wheel compacting mechanism is rotated to move the centering pull bar and centering pressure bar and open the elastic centering expanding sleeve to center the first hole system, and the variable-pitch cam mechanism and the auxiliary centering mechanism are inserted in two sides of a second hole system of the part to be measured. The handle is rotated to drive the cam to rotate, so that the rim of the cam is tangent to the excircle of the elastic centering expanding sleeve, and angle scales corresponding to different center distances of the first hole system and the second hole system are radially marked on the end face of the servo-actuated eccentric cam. The actual center distance of the intersecting holes of the part to be measured can be rapidly obtained by observing the angle graduation lines, and rapid and accurate measurement is realized.

The invention relates to enzyme method measuring sodium ion content method and its diagnosis kit. It is adopted galactosidase reaction colorimetry to use sodium ion to activate beta-galactosidase enzymolysis o-nitrophenol group-beta-D- pyranoside galactose to make 405nm dominant wavelength absorbance rise. Its absorbance amplitude of fluctuation is proportional to sample sodium ion. The method has high specificity, good accuracy. The diagnosis kit can be made into bi-agent to reduce cross influence. The method can be quickly measured by ultraviolet / visible light analyzer or semi / full automatic biochemical analysis. Its measuring cost is low; and it is convenient for generalization and application.

A method for determining concentration of homotype cysteine includes synthesizing cystathionine B synthase, homotype cysteine and serine to be cystathionine; cracking cystathionine by cystathionine B lyase to change it back to be homotype cysteine and to generate ammonia and pyruvic acid; using some chemicals to act on ammonia to let reduction coenzyme be oxidized to be oxidation coenzyme for determining out absorbance dropping speed of reduction coenzyme at 340nm then estimating out concentration size of homotyp cysteine. Its diagnosis kit can be prepared in double dosages for decreasing cross influence of various compositions.

The invention discloses a multi-system integrated intelligent substation relay protection dynamic regular inspection method. The method comprises the steps: setting an automatic inspection period, anautomatic regular inspection period and an early warning period; initializing regular inspection counting time of each inspection item as initial timing time; and carrying out automatic inspection oneach inspection item in the automatic inspection period to obtain corresponding automatic inspection results; and carrying out result confirmation according to the automatic inspection results and resetting regular inspection counting time. According to the invention, in the automatic regular inspection period and before the early warning period, when inspection items all act correctly, and automatic inspection results are normal, the relay protection device can perform regular inspection completely without stopping, and the regular inspection period is prolonged; if the automatic inspection results of the inspection items are no result all the time, the relay protection device enters the early warning period and needs to be stopped for regular inspection, and only the inspection items which do not act can be inspected according to the inspection items, so that the number of the regular inspection items can be reduced to the minimum, the power failure time is shortened, and the regularinspection workload is reduced.

The invention discloses a magnetic steel detection device. The magnetic steel detection device is composed of an upper pressing part and a supporting part, wherein the upper pressing part comprises an upper pressing column, a vertical column, a connecting shaft and an upper support, wherein a handle is connected to the connecting shaft, one side of the upper support is fixedly provided with a pressure display controller, and the vertical column and the supporting part are fixed on a base. The magnetic steel detection device provided by the invention has the advantages that the structure is simple, the detection process and the operation are convenient, the detection result is credible, and the detection work efficiency of work efficiency is improved.

The invention relates to a kind of enzyme colorimetric method and a method of the coupling method measuring the density of citric acid and a diagnosis reagent box applying the citrate synthetase and coupling malic dehydrogenase enzymatic reaction continues monitoring method / ratio colorimetric method. The reaction of citrate synthetase enzymolysis citric acid generates oxaloacetic acid and then through the effect of coupling malic dehydrogenase at last oxidizes the reduced coenzyme (there is a absorption peak at the site of 340nm) becoming the coenzyme (there is a absorption peak at the site of 340nm) so we can assay the degree / speed of the fall-way absorbance at the place of 340nm. It can measure and calculate the density of citric acid by measuring the degree / speed of the fall-way absorbance at the place of 340nm. The method has high specificity and it is not polluted by the endogenous and exogenous object and the test result is precise and accurate. The invention can get the array result by the ultraviolet / visible light analytic instrument so it is convenience to extend and apply.

The invention discloses a coal face physical similar simulation support having a setting load. The coal face physical similar simulation support comprises a support frame, a support column, a force measuring sensing system and a display meter; the support frame comprises a pedestal, a connecting rod, a shield plate and a top guard plate; the force measuring sensing system is fixed to the pedestal in the support frame; the force measuring sensing system is electrically connected with the display meter via a signal transmission line; and the support column is a length-adjustable telescopic column, a lower end of the support column is fixed to an upper plate surface of the force measuring sensing system, and an upper end of the support column is fixed to a lower surface of the top guard plate of the support frame. According to the coal face physical similar simulation support having the setting load, test precision can be improved, the engineering field can be simulated truly, and result reliability can be achieved.

The invention discloses primers, a probe and a kit for detecting the BRCA1 gene expression. The nucleotide sequences of upstream and downstream primers of BRCA1 gene are respectively represented by the SEQ ID No. 1-2. The nucleotide sequence of Taqman fluorescence probe of BRCA1 gene is represented by the SEQ ID No.3. The invention also discloses a detection kit composed of the primers and probe mentioned above, and an application method of the kit. The provided kit can detect samples in different forms such as fresh frozen tissues, paraffin embedded samples, and the like, and has the advantages of high sensitivity, high specificity, and wide application range. The kit comprises premix, a sample can be added directly, a sample does not need to be mixed with enzyme, the operation is convenient and simple, the judgment method becomes more scientific and reasonable, the error generated during the experiment process is reduced, the precision is high, furthermore, the detection speed of the detection kit is quick, and the detection result can be obtained within two hours.

The invention discloses a rapid and quantitative detection method of paraffin doped in lard. According to the detection method, through detection on 13C / 12C values of a to-be-detected sample and paraffin-undoped lard in the existing database, and a delta 13C value of the to-be-detected sample is calculated and comparatively analyzed to judge whether the to-be-detected sample is doped with the paraffin; on the basis, the paraffin content of the to-be-detected sample can be calculated according to a formula. By virtue of a significant difference between 13C / 12C values of carbon stable isotopes of the lard and the paraffin, the purpose of rapidly detecting whether the to-be-detected sample is doped with the paraffin is achieved, and a result is real; in addition, the amount of the doped paraffin can be quantitatively detected, and a quantitative analysis result is reliable; with the detection method provided by the invention, the defects of the conventional detection method of the paraffin doped in the lard can be overcome, the detection efficiency is high, the batch detection can be achieved, the detection analysis cost is low, the detection result is not significantly different from that obtained in the conventional detection method, the result is credible, the pollution to a detection instrument is small and the application prospect is wide.

The invention relates to measuring method for carbon dioxide content and diagnosing kit. The invention directly uses carbon dioxide( bicarbonate radical) and pyruvic acid to produce malic acid by decarboxylation of malic dehydrogenase and oxidizes reduced coenzyme into oxidized type coenzyme, measuring the decent rate of dominant wavelength 340nm absorbance could quantitatively response the carbon dioxide content in sample, thus, the strength of bicarbonate radical could be extrapolated. The method has high specificity, has no pollution from inside and outside material, and accurate test result. The diagnose kit is made up into bi-agent or tri-agent that could reduce cross influence from each component and keeps stability of the agent that is convenient for long-term storage. The method could quickly test on commonness ultraviolet / visible light, analysis or semi-transfer / full automatically biochemical study instrument, and doesn't need particularity or extra instrument. The testing cost is cheap, and is convenient for popularization.

The invention discloses nucleic acid and a kit used for detecting polymorphism of COX-1, COX-2 and GPIIIa genes as well as detecting method for detecting polymorphism of the COX-1, COX-2 and GPIIIa genes, and the method is strong in specificity, is high in sensitivity, is high in accuracy and is simple to operate. The detected results can provide guidance meaning for aspirin resistance. The detecting method provided by the invention adopts completely closed-tube operation, is simple to operate, is convenient and quick, obtains the detected results by directly detecting a fluorescence signal value in a PCR process, PCR post-treatment or electrophoresis detection is not needed, the problems such as easy pollution and false positive results in the conventional PCR technology are overcome, the nonspecific amplification problem can be effectively avoided, and therefore, the nucleic acid, the kit and the method are suitable for large-scale sample detection.

The invention provides a detection method for an upstream pressure of a Venturi tube. The method comprises the following steps: acquiring the upstream pressure of the Venturi tube and a pressure behind a throttle valve and acquiring a pressure difference value of the upstream pressure of the Venturi tube and the pressure behind the throttle valve when an engine is in a stable working condition andan EGR valve is closed; and when the pressure difference value is smaller than a current judgment threshold value, determining that the detection result of the upstream pressure of the Venturi tube is reliable. According to the detection method provided by the invention, the engine is not required to be in an idle state, the upstream pressure of the Venturi tube is also detected in a process thatthe engine operates, and the effectiveness and the accuracy of the detection result are guaranteed, so that the control accuracy of the EGR is further improved. The invention also provides a detection system for the upstream pressure of the Venturi tube.

The active feature inspection for acetylglucosamidase and its diagnostic agent box uses its fostering of continuous monitoring method, enzymolysising dual chlorineor chlorine acetylglucosamidase reaction, through the absorbance elevating speed of the 405nm, inspecting its active feature. The agent box comprises the bumper solution, synthesized nitrobenzene betaacetylglucosamidase and stabilizer to make dual agent by the diagnostic agent box to reduce cross impact of all kinds of components. Through visible light analyzer, the result is precise, free from outside contamination, easy for promotion.

The invention discloses a gas sampling enrichment device based on nano-fibers, comprising a gas sampling device (1) and a gas enrichment device (2) and characterized in that the gas enrichment device (2) is in control communication with the gas sampling device (1), and the nano-fibers (21) in which gas components are enriched are arranged in the gas enrichment device (2). The device can accurately fix the collection quantity of gas to enable a detection result to be more reliable, can reduce the collection quantity of the gas, in particular gas with less content, can save the time, the labor and the cost and does not need any power system.

The invention discloses a method for in-situ detection of the migration quantity of a fluorescent whitening agent; a fluorescence content value A of a to-be-measured sample which is not used is measured, a fluorescence content value B is measured again after the to-be-measured sample is used, and the migration quantity of the fluorescent whitening agent=(A-B) / A*100%. The method comprises that firstly, the concentration of the fluorescent whitening agent at a certain part of the to-be-measured sample is detected when the to-be-measured sample does not make contact with a human body, then the concentration of the fluorescent whitening agent at the part is detected again after the to-be-measured sample makes contact with the human body, the concentrations of the fluorescent whitening agent before and after contact with the human body are compared, and thus the migration quantity of the fluorescent whitening agent can be obtained; the detection method can most truly and directly reflect the migration quantity of the fluorescent whitening agent. The method is fast in detection speed, and can provide a reliable detection method for on-site detection of the migration quantity of the fluorescent whitening agent in the sample.

The invention provides a heterophoria objective detection method and system. Eye position real-time monitoring is adopted, the measurement process is simple, a detected person does not need to participate in adjustment, the detection result is objective, reliable and more credible, and the detection process is simple and fast.

The invention discloses a surface roughness testing method and a tester. The testing method comprises the steps of placing a friction assembly on a tested concrete surface so that the friction assembly rotates or slides on the tested concrete surface, measuring physical quantities such as rotating speed, torque, speed and resistance of the friction assembly in movement, calculating the characteristic quantity of the surface roughness or estimating the surface roughness of the tested concrete according to the measured physical quantities and a corresponding calculating formula. The tester for realizing the method comprises a body, a supporting seat and the friction assembly, wherein the friction area of the friction assembly is annular or rectangular. The testing method and the tester of the invention are used for testing and evaluating the concrete surface roughness and have advantages of clear principle, trustable result, wide application range and convenient operation.

The invention discloses a nucleic acid, kit and method for rapid detection of C3435T polymorphism of an ABCB1 gene. The nucleic acid comprises detection primers and a detection probe, wherein the detection primers comprise a wild downstream primer, a mutant downstream primer and a common upstream primer; a nucleotide sequence of the wild downstream primer is as shown in SEQ ID No.1, a nucleotide sequence of the mutant downstream primer is as shown in SEQ ID No.2, a nucleotide sequence of the common upstream primer is as shown in SEQ ID No.3, and a nucleotide sequence of the detection probe is as shown in SEQ ID No.4. The kit and method for real-time fluorescent quantitative PCR rapid detection of C3435T polymorphism of the ABCB1 gene have the advantages of being convenient to use, simple to operate and high in automatic degree, greatly simplifying the operation process and reducing pollution in the operation process, and the detection effect is good; the kit has the characteristics of high sensitivity, specificity, accuracy and precision.

The invention provides a multifunctional sputum suction device for respiratory medicine. The multifunctional sputum suction device comprises a sputum suction catheter and a cup body, wherein the sputum suction catheter is connected to the upper part of the cup body and comprises a sputum reducing tube with a sputum diluting function, a flow meter with a sputum flow counting function and a hose forconnecting the sputum reducing tube with the flow meter, a sputum collection and detection bottle used for collecting sputum and detecting types of bacteria and viruses contained in the sputum is arranged in the cup body and communicates with the sputum suction catheter through a sputum channel in the cup body, and a driving device is further arranged in the cup body and used for enabling the sputum channel to be in a low-pressure state, so that the sputum suction catheter can suck the sputum. According to the multifunctional sputum suction device, microorganisms contained in sucked sputum can be detected under the condition that the sucked sputum is not polluted by the external environment, meanwhile, the sputum amount of a patient is counted, and better treatment can be conducted according to sputum information.

The invention provides a telomerase activity detection kit, and further provides a method for detecting the telomerase activity of a sample by using the detection kit. The telomerase activity detection kit comprises a telomerase extension system component, a magnetic bead system component and a signal generation system of a signal enzyme and a substrate. According to the detection kit and the detection method, a triple-mode system for visual and portable detection of the telomerase activity based on a magnetic bead-enzyme system is achieved, and the advantages of magnetic bead magnetic separation and enzymatic reaction signal amplification are combined. Two different enzymes including glucosidic bond hydrolase and phosphate ester bond hydrolase and three corresponding different enzyme substrates can be used for simultaneously carrying out parallel detection on the same sample by three different detection principle-based methods including a fluorescence detection method, a colorimetricdetection method and an ATP detection method, so that a false positive result and a false negative result can be avoided, the detection result is more credible, and the detection kit and detection method can be used for rapid determination of the telomerase activity and determination of single cell level.

The invention relates measuring method of monoamine oxidase activity and its diagnostic kit, comprising the following steps: using the benzylamine, p-toluidine red-ª‰-azo naphthol, butyl amine, amyl amine, ª‰-phenylethylamine, tyramine and other aminated compounds as substrate, it reacted with monoamine oxidase to produce auricome, then auricome carried out coupling reaction with hydrogen peroxidase and aldehyde dehydrogenase, carrying out the reaction to turn the oxidation type coenzyme to reduction type coenzyme, detecting the ascending velocity of absorbance of main wavelength 340nm to reflect the activity of monoamine oxidase in sample by definite quantity. The method has high specificity, good accuracy. The diagnostic kit is made to double agents or tri-agent to reduce the across impact and keep the agent stability and be good for long term storage. The method can be rapidly detected by the ultra-violet / visible light analysis meter or semi-automatic / full-automatic biochemical analysis meter, so the method is easy to spread and use.