38results about How to "The results are objective and reliable" patented technology

The invention relates to the field of microorganism assay, and in particular relates to a candida glabrata PCR (polymerase chain reaction) assay kit and a method. The kit comprises a PCR solution, wherein the PCR solution comprises primers A and a fluorescent probe A; the primers are divided into a forward primer A and a reverse primer A; the nucleotide sequence of the forward primer A is shown in the SEQ ID NO.1; the nucleotide sequence of the reverse primer A is shown in the SEQ ID NO.2; and the nucleotide sequence of the fluorescent probe A is shown in the SEQ ID NO.3. The kit has the advantages of high sensitivity and specificity, stability, timeliness, convenience in operation and the like.

The invention relates to a test method, in particular to a method for testing the oxygen permeability of a packaging bag. In the present invention, the packaging material to be tested and several packaging materials with known oxygen permeability are cut into the same size, each of which is sealed with the same brand deoxidizer of the same specification, fixed at the bottom of the container and placed upside down in the container containing the solution, adjusted and made At the beginning of the test, the value of the liquid level scale at the top is consistent. After a certain period of time, compare the packaging material to be tested with other known packaging material detection devices, that is, the liquid level in the container, so as to qualitatively judge the oxygen permeability of the packaging material to be tested. . The present invention can detect the oxygen permeability rate of the packaging material without resorting to complex and expensive precision instruments, and the test operation is simple and difficult, and the result is objective and reliable, which saves investment in machinery equipment and sites for enterprises, reduces economic burden, and has good advantages. Promote prospects.

The invention relates to the field of microbe detection, in particular to a polymerase chain reaction (PCR) fluorescence detection kit for candida albicans. According to the technical scheme, the fluorescence detection kit for the candida albicans comprises a PCR reaction solution; the PCR reaction solution comprises primers A and a fluorescence probe A; the primers are divided into an upstream primer A and a downstream primer A; and the nucleotide sequence of the upstream primer A is shown as SEQ ID NO.1, the nucleotide sequence of the downstream primer A is shown as SEQ ID NO.2, and the nucleotide sequence of the fluorescence probe A is shown as SEQ ID NO.3. The kit has the advantages of high sensitivity and specificity, stability, timeliness, convenience for operation and the like.

A rat cold-heat approach behavior measurer belongs to animal experiment instrument and equipment, and is mainly used in the research of the cold-heat constitution of a rat, a cold-heat tendency under a pathologic condition and the influence of traditional Chinese medicine cold-heat drug nature on the cold-heat tendency behavior of a rat so as to carry out a research in the four nature theory of traditional Chinese medicine. The invention is realized through adopting the following steps that: a heat case 1, a room temperature case 12 and a cold case 10 are arranged inside an integral case shell 15; the heat case and the cold case are respectively provided with a heating element inside, a cooling element, a temperature controller, a photoelectric switch, a time accumulation timer, a 220 V power supply and an AC / DC switching power supply; all circuits are arranged between the integral case shell on one hand and the heat case, the room temperature case and the cold case on the other hand;and one outer side wall of the integral case shell is provided with a control panel 9 so as to operate the entire measurer.

The invention relates to the field of microorganism assay, and in particular relates to a candida tropicalis PCR (polymerase chain reaction) assay kit. The kit comprises a PCR solution, wherein the PCR solution comprises primers and a fluorescent probe A; the primers are divided into a forward primer A and a reverse primer A; the nucleotide sequence of the forward primer A is shown in the SEQ ID NO.1; the nucleotide sequence of the reverse primer A is shown in the SEQ ID NO.2; and the nucleotide sequence of the fluorescent probe A is shown in the SEQ ID NO.3. The kit has the advantages of high sensitivity and specificity, stability, timeliness, convenience in operation and the like.

The invention belongs to plant physiology and particularly relates to a method for identifying low-temperature resistance of wild cotton in a seedling stage. The method comprises the following steps: 1) sulfuric acid delinting and incision treatment on seeds; 2) germination accelerating treatment; 3) seedling culture: common seedlings in a 3-5-leaf stage with consistent growth vigor are selected; 4) low-temperature stress treatment: low-temperature stress treatment closer to natural conditions is selected; 5) low-temperature resistance level classification: grading is performed according to damaged conditions of cotton seedlings, and resistance levels of the wild cotton are classified. According to the method, severe low-temperature stress treatment is performed in the seedling stage when the wild cotton is most sensitive to low temperature, so that a low-temperature-resistant cotton material truly with higher resistance level can be screened out; grading is performed according to damaged conditions of cotton leaves, objective classification is performed, and errors caused by human factors are reduced greatly. The method is not limited by seasons, can be used for identifying mass materials and is easy to popularize and use.

The invention discloses a BMP-2 antibody and application to detection of content of BMP-2 protein in a bone repairing material thereof. In the application, the BMP-2 antibody is an antibody secreted by an rhBMP-2 monoclonal antibody cell strain 44F5 which is preserved in China General Microbiological Culture Collection Center; the preservation number of the rhBMP-2 monoclonal antibody cell strain is CGMCC No.12257. Experiments show that the BMP-2 antibody is high in specificity, high in sensitivity, high in accuracy and high in stability when the BMP-2 antibody is used for detecting the content of the BMP-2 protein in the bone repairing material; the recovery ratio (which is a ratio of a BMP-2 content detection value to a BMP-2 content actual value) is 106.4%; the degree of precision is 5.2%; a method established for detecting the content of the BMP-2 protein in the bone repairing material or the bone tissue is relatively stable, is high in accuracy and is suitable for detecting the content of the BMP-2 protein in the bone repairing material as a conventional method.

The invention relates to a rat cold hot drink preference determinator. The invention belongs to the animal experimental apparatus field and is mainly used for investigating whether a rat likes drinking hot water or cold water and providing an objective quantitative index, thereby investigating the cold or hot body state of the rat or investigating whether a pathological attribute during a diseaseis a hot property symptom or a cold property symptom. The invention is provided with a water bottle connected with a water outlet pipe, wherein the latter provides drinking water for the rat. A switching power supply provides a power supply of 5V for a refrigeration piece, a temperature control resistor can sense the temperature of hot water to further control the working status of the refrigeration piece and consequently control the water temperature, and a water collection barrel collects the water which is carelessly dropped by the drinking rat. The observation of the reduced water amount in the water bottle and the water collection amount in the water collection barrel can obtain the water intake amount of the rat, thereby judging the cold hot drink preference of the rat.

The invention discloses a pine wood nematode PCR (polymerase chain reaction) detection kit. The detection kit comprises a PCR reaction solution containing a primer A and a fluorescent probe A, wherein the primer A is divided into an upstream primer A and a downstream primer A; the nucleotide sequence of the upstream primer A is 5'-CCGTTTGGTGATGTTGTTTCA-3'; the nucleotide sequence of the downstream primer A is 5'-GCAAGGCTTCACGACGAA-3'; and the nucleotide sequence of the fluorescent probe A is 5'-FAM-CTCAACAAACCGCAGCCAGGACTC-BHQ-3'. The invention further discloses a pine wood nematode PCR detection method. The detection kit disclosed by the invention has the advantages of good DNA (deoxyribonucleic acid) extraction effect, simple operation, short consumed time, objective and reliable results, high sensitivity, high specificity and the like.

The invention discloses a method for testing the spraying effect of a sprayer by using the spores of Nomura spp., which belongs to the technical field of performance testing of agricultural production appliances. Add Tween80 to the water suspension containing Nomura spores, ultrasonically vibrate, add water and mix evenly to obtain a test solution. Use the tested sprayer to spray the test liquid on the plants of the same species at different distances and angles towards the front and back of the leaves. After the leaves of the plants dry naturally, collect the sprayed leaves of the plants, rinse them with pure water, and take them out. The solution was examined and counted under a microscope to calculate the number of spores per unit area. The method of comparing the spraying effects of different sprayers is: under the same spraying conditions, the greater the number of attached spores, the better the spraying efficiency and uniformity. Positive effect: In the actual production process, the verification test of the three-dimensional spray effect is carried out, and the result is more objective and reliable.

The invention discloses a method for testing BNP (Brain Natriuretic Peptide) activity by using a GCA stable transfection cell line and establishes a novel BNP bioactivity testing method. GCA which is a main acceptor of BNP is an adenylate cyclase precursor and can catalyze GTP (guanosine triphosphate) to generate cGMP after being activated by the BNP and then exerts a biological effect. Wield 293 cell has low GCA expression level and low reactivity to BNP. In the method disclosed by the invention, a stable cell line 293GCAC3 with BNP acceptor (GCA) overexpression level is structured by transgenosis, the content change of cGMP secreted into cell supernatant by the 293GCAC3 stimulated by the BNP is detected to quantify the activity of the BNP. The method comprises the following specific testing steps of: laying cells on a 96-pore plate and culturing for 16-18 hours; adding the BNP for stimulating for 90 minutes; and sucking cell supernatant and detecting the content of cGMP with competitive ELISA (enzyme-linked immuno sorbent assay). The method is simple to operate, has sensitive response and small variability and is significant for the quality control and clinical application of the BNP.

The invention discloses an ischemic stroke treatment monitoring system based on image information, which includes: a clinical image processing module, which is used to realize the import, storage, format conversion, grouping, and image standardization processing of clinical cerebral blood perfusion images, and Generate cerebral blood perfusion template; image analysis module before and after treatment, used to realize comparative analysis of time series images of cerebral blood perfusion before and after clinical treatment, and calculate and obtain quantitative parameters for curative effect evaluation; treatment monitoring module, used to select the Quantitative parameters, and establish the relationship between the quantitative parameters and the recovery level of traditional clinical assessment, generate an expert judgment model for curative effect evaluation, and monitor the treatment process of ischemic stroke based on the expert judgment model.