38results about How to "The results are objective and reliable" patented technology

The invention relates to the field of microorganism assay, and in particular relates to a candida glabrata PCR (polymerase chain reaction) assay kit and a method. The kit comprises a PCR solution, wherein the PCR solution comprises primers A and a fluorescent probe A; the primers are divided into a forward primer A and a reverse primer A; the nucleotide sequence of the forward primer A is shown in the SEQ ID NO.1; the nucleotide sequence of the reverse primer A is shown in the SEQ ID NO.2; and the nucleotide sequence of the fluorescent probe A is shown in the SEQ ID NO.3. The kit has the advantages of high sensitivity and specificity, stability, timeliness, convenience in operation and the like.

The invention relates to a test method, in particular to a method for testing the oxygen permeability of a packaging bag. In the present invention, the packaging material to be tested and several packaging materials with known oxygen permeability are cut into the same size, each of which is sealed with the same brand deoxidizer of the same specification, fixed at the bottom of the container and placed upside down in the container containing the solution, adjusted and made At the beginning of the test, the value of the liquid level scale at the top is consistent. After a certain period of time, compare the packaging material to be tested with other known packaging material detection devices, that is, the liquid level in the container, so as to qualitatively judge the oxygen permeability of the packaging material to be tested. . The present invention can detect the oxygen permeability rate of the packaging material without resorting to complex and expensive precision instruments, and the test operation is simple and difficult, and the result is objective and reliable, which saves investment in machinery equipment and sites for enterprises, reduces economic burden, and has good advantages. Promote prospects.

The invention discloses a method for testing BNP (Brain Natriuretic Peptide) activity by using a GCA stable transfection cell line and establishes a novel BNP bioactivity testing method. GCA which is a main acceptor of BNP is an adenylate cyclase precursor and can catalyze GTP (guanosine triphosphate) to generate cGMP after being activated by the BNP and then exerts a biological effect. Wield 293 cell has low GCA expression level and low reactivity to BNP. In the method disclosed by the invention, a stable cell line 293GCAC3 with BNP acceptor (GCA) overexpression level is structured by transgenosis, the content change of cGMP secreted into cell supernatant by the 293GCAC3 stimulated by the BNP is detected to quantify the activity of the BNP. The method comprises the following specific testing steps of: laying cells on a 96-pore plate and culturing for 16-18 hours; adding the BNP for stimulating for 90 minutes; and sucking cell supernatant and detecting the content of cGMP with competitive ELISA (enzyme-linked immuno sorbent assay). The method is simple to operate, has sensitive response and small variability and is significant for the quality control and clinical application of the BNP.