BMP-2 antibody and application to detection of content of BMP-2 protein in bone repairing material thereof

Abstract

The invention discloses a BMP-2 antibody and application to detection of content of BMP-2 protein in a bone repairing material thereof. In the application, the BMP-2 antibody is an antibody secreted by an rhBMP-2 monoclonal antibody cell strain 44F5 which is preserved in China General Microbiological Culture Collection Center; the preservation number of the rhBMP-2 monoclonal antibody cell strain is CGMCC No.12257. Experiments show that the BMP-2 antibody is high in specificity, high in sensitivity, high in accuracy and high in stability when the BMP-2 antibody is used for detecting the content of the BMP-2 protein in the bone repairing material; the recovery ratio (which is a ratio of a BMP-2 content detection value to a BMP-2 content actual value) is 106.4%; the degree of precision is 5.2%; a method established for detecting the content of the BMP-2 protein in the bone repairing material or the bone tissue is relatively stable, is high in accuracy and is suitable for detecting the content of the BMP-2 protein in the bone repairing material as a conventional method.

Description

technical field [0001] The invention relates to a BMP-2 antibody in the field of biotechnology and its application in detecting the BMP-2 protein content in bone repair materials. Background technique [0002] Bone morphogenetic protein (BMP) is a hydrophobic protein secreted by bone matrix and belongs to the transforming growth factor (TGF-β) superfamily member. It was first proposed by Urist et al. in 1965, defined in 1972, and defined in 1982. An active protein isolated for the first time from decalcified bone matrix extract. The BMP family includes BMP-1, 2, 4, 6, 7, 9, 12, 14, etc., among which BMP-2 can obviously induce undifferentiated mesenchymal stem cells and fibroblasts to differentiate into osteoblasts and can Regulates the differentiation of osteoblasts and chondrocytes, induces ectopic bone formation and promotes fracture healing. BMP-2 is the initiation factor of osteogenesis, which can accelerate bone reconstruction. It is the ideal target gene for bone rep...

AI Technical Summary

Problems solved by technology

In the prior art, the method of first eluting BMP-2 in the bone material and then measuring the eluent is generally adopted. On the one hand, the elution of BMP-2 is incomplete or difficult to elute, resulting in inaccurate detection; Determination of BMP-2 content in the eluent, the eluent will interfere with the detection results

Or calculate by measuring the BMP-2 protein concentration of the solution before and after the combination, but the BMP-2 content cannot be accurately measured after the finished product is made

After this method is combined, the protein concentration is too low, which is lower than the detection limit of the relevant detection method. Not only is the operation cumbersome, but the error is relatively large.

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