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Telomerase activity detection kit and telomerase activity detection method

A technology of activity detection and telomerase, applied in the field of biochemical detection

Pending Publication Date: 2021-02-26
THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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Problems solved by technology

[0004] Despite the above advances in telomerase activity assay technology, there is still room for improvement

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  • Telomerase activity detection kit and telomerase activity detection method
  • Telomerase activity detection kit and telomerase activity detection method
  • Telomerase activity detection kit and telomerase activity detection method

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Embodiment Construction

[0044] The present invention will be further described in detail below through specific embodiments and in conjunction with the accompanying drawings.

[0045] figure 1 A schematic diagram showing the detection principle of the triple mode of the telomerase activity detection kit of the present invention and the telomerase activity detection method using the kit, combined below figure 1 Briefly describe the detection principle.

[0046] Magnetic beads (MB) were modified with streptavidin (SA) to form streptavidin-modified magnetic beads (MB-SA). The first oligonucleotide sequence is modified with biotin to form a biotin-modified first oligonucleotide (bio-oligo1). The second oligonucleotide sequence is modified with biotin to form a biotin-modified second oligonucleotide (bio-oligo 2). The second oligonucleotide sequence is designed to at least partially hybridize to the first oligonucleotide sequence. Galactosidase (Gala) was modified with streptavidin (SA) to form strept...

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Abstract

The invention provides a telomerase activity detection kit, and further provides a method for detecting the telomerase activity of a sample by using the detection kit. The telomerase activity detection kit comprises a telomerase extension system component, a magnetic bead system component and a signal generation system of a signal enzyme and a substrate. According to the detection kit and the detection method, a triple-mode system for visual and portable detection of the telomerase activity based on a magnetic bead-enzyme system is achieved, and the advantages of magnetic bead magnetic separation and enzymatic reaction signal amplification are combined. Two different enzymes including glucosidic bond hydrolase and phosphate ester bond hydrolase and three corresponding different enzyme substrates can be used for simultaneously carrying out parallel detection on the same sample by three different detection principle-based methods including a fluorescence detection method, a colorimetricdetection method and an ATP detection method, so that a false positive result and a false negative result can be avoided, the detection result is more credible, and the detection kit and detection method can be used for rapid determination of the telomerase activity and determination of single cell level.

Description

technical field [0001] The invention relates to the technical field of biochemical detection, in particular to a telomerase activity detection kit and a method for using the detection kit to detect telomerase activity in a sample to be tested. Background technique [0002] Telomerase, which adds the telomere repeat sequence (TTAGGG)n to the ends of telomeres, is essential for telomere maintenance and protection 1-4 . Telomerase has been found to be overexpressed in approximately 85-90% of human cancer cells such as bladder cancer, uterine cancer, gastric cancer, esophageal cancer, breast cancer, thyroid cancer, colorectal cancer, laryngeal squamous cell carcinoma 5-8 , whereas telomerase activity is absent in normal tissues. Therefore, telomerase is generally regarded as an important biomarker of human malignancies 9-12 . Simple, rapid and sensitive detection of telomerase activity is of great significance for early diagnosis of cancer. [0003] Various strategies have ...

Claims

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Application Information

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IPC IPC(8): C12Q1/48
CPCC12Q1/48
Inventor 马英新黄卫人
Owner THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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