Process for determining content of carbon dioxide and kit for diagnosing carbon dioxide therefor
A diagnostic kit and technology of carbon dioxide, which is applied in the field of medical testing and measurement, can solve the problems of large measurement errors and achieve the effects of high accuracy, low test cost and stable reagents
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Embodiment 1
[0046] Embodiment one (single dose)
[0047] Prepare the carbon dioxide diagnostic kit according to the following ingredients and dosage:
[0048] Glycine buffer 80mmol / l,
[0049] Pyruvate 5mmol / l,
[0050] thio-NADH 0.2mmol / l,
[0051] Malate dehydrogenase 6000U / l,
[0052] Ethylene glycol 50% (accounting for the total volume of the reagent).
[0053] Set on the automatic biochemical analyzer (Hitachi-7080): temperature 37°C, reaction time 10 minutes, test main wavelength 340nm, test secondary wavelength 405nm or more, the volume ratio of the measured carbon dioxide sample to the reagent is 1:25, the reaction The direction is negative reaction (absorbance decreases, the same below).
[0054] Add the sample and the prepared single agent, and the two are automatically mixed inside the analyzer, and the decrease of the absorbance at 340nm is detected and recorded. According to the rate method, end point method and other methods, the content of car...
Embodiment 2
[0055] Embodiment two (two doses)
[0056] Prepare the carbon dioxide diagnostic kit according to the following ingredients and dosage:
[0057] Reagent I——
[0058] Imidazole ~ hydrochloric acid buffer 100mmol / l,
[0059] Pyruvate 13mmol / l,
[0060] NADPH 0.25mmol / l,
[0061] Glycerin 20mmol / l;
[0062] Reagent II——
[0063] Imidazole ~ hydrochloric acid buffer 100mmol / l,
[0064] Malate dehydrogenase 9000U / l,
[0065] Ethylene glycol 50% (accounting for the total volume of reagent II).
[0066] Set on the automatic biochemical analyzer (Hitachi-7080): temperature 30°C, reaction time 15 minutes, test main wavelength 340nm, test secondary wavelength 405nm or more, the ratio of the total volume of the measured carbon dioxide sample to reagent I and reagent II is 1:25, the ratio of the amount of reagent I to reagent II is 4:1, and the reaction direction is negative reaction.
[0067] Add the sample and reagent I first, then add reagent...
Embodiment 3
[0068] Embodiment three (three doses)
[0069] Prepare the carbon dioxide diagnostic kit according to the following ingredients and dosage:
[0070] Reagent I——
[0071] Triethanolamine buffer 120mmol / l,
[0072] thio-NADPH 0.3mmol / l,
[0073] Propylene glycol 20mmol / l;
[0074] Reagent II——
[0075] Triethanolamine buffer 120mmol / l,
[0076] Pyruvate 20mmol / l,
[0077] Propylene glycol 20mmol / l;
[0078] Reagent III——
[0079] Triethanolamine buffer 120mmol / l,
[0080] Malate dehydrogenase 12000U / l,
[0081] Ethylene glycol 50% (accounting for the total volume of reagent III).
[0082] Set on the automatic biochemical analyzer (Hitachi-7080): temperature 25°C, reaction time 20 minutes, test main wavelength 340nm, test secondary wavelength 405nm or more, the total amount of the measured carbon dioxide sample and reagent I, reagent II and reagent III The volume ratio is 1:25, the dosage of reagent I, reagent II and reagent...
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