Process for determining content of carbon dioxide and kit for diagnosing carbon dioxide therefor

A diagnostic kit and technology of carbon dioxide, which is applied in the field of medical testing and measurement, can solve the problems of large measurement errors and achieve the effects of high accuracy, low test cost and stable reagents

Inactive Publication Date: 2006-06-14
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The direct method is the most widely used titration method, but due to the influence of many factors, the measurement error is large

Method used

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  • Process for determining content of carbon dioxide and kit for diagnosing carbon dioxide therefor
  • Process for determining content of carbon dioxide and kit for diagnosing carbon dioxide therefor
  • Process for determining content of carbon dioxide and kit for diagnosing carbon dioxide therefor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment one (single dose)

[0047] Prepare the carbon dioxide diagnostic kit according to the following ingredients and dosage:

[0048] Glycine buffer 80mmol / l,

[0049] Pyruvate 5mmol / l,

[0050] thio-NADH 0.2mmol / l,

[0051] Malate dehydrogenase 6000U / l,

[0052] Ethylene glycol 50% (accounting for the total volume of the reagent).

[0053] Set on the automatic biochemical analyzer (Hitachi-7080): temperature 37°C, reaction time 10 minutes, test main wavelength 340nm, test secondary wavelength 405nm or more, the volume ratio of the measured carbon dioxide sample to the reagent is 1:25, the reaction The direction is negative reaction (absorbance decreases, the same below).

[0054] Add the sample and the prepared single agent, and the two are automatically mixed inside the analyzer, and the decrease of the absorbance at 340nm is detected and recorded. According to the rate method, end point method and other methods, the content of car...

Embodiment 2

[0055] Embodiment two (two doses)

[0056] Prepare the carbon dioxide diagnostic kit according to the following ingredients and dosage:

[0057] Reagent I——

[0058] Imidazole ~ hydrochloric acid buffer 100mmol / l,

[0059] Pyruvate 13mmol / l,

[0060] NADPH 0.25mmol / l,

[0061] Glycerin 20mmol / l;

[0062] Reagent II——

[0063] Imidazole ~ hydrochloric acid buffer 100mmol / l,

[0064] Malate dehydrogenase 9000U / l,

[0065] Ethylene glycol 50% (accounting for the total volume of reagent II).

[0066] Set on the automatic biochemical analyzer (Hitachi-7080): temperature 30°C, reaction time 15 minutes, test main wavelength 340nm, test secondary wavelength 405nm or more, the ratio of the total volume of the measured carbon dioxide sample to reagent I and reagent II is 1:25, the ratio of the amount of reagent I to reagent II is 4:1, and the reaction direction is negative reaction.

[0067] Add the sample and reagent I first, then add reagent...

Embodiment 3

[0068] Embodiment three (three doses)

[0069] Prepare the carbon dioxide diagnostic kit according to the following ingredients and dosage:

[0070] Reagent I——

[0071] Triethanolamine buffer 120mmol / l,

[0072] thio-NADPH 0.3mmol / l,

[0073] Propylene glycol 20mmol / l;

[0074] Reagent II——

[0075] Triethanolamine buffer 120mmol / l,

[0076] Pyruvate 20mmol / l,

[0077] Propylene glycol 20mmol / l;

[0078] Reagent III——

[0079] Triethanolamine buffer 120mmol / l,

[0080] Malate dehydrogenase 12000U / l,

[0081] Ethylene glycol 50% (accounting for the total volume of reagent III).

[0082] Set on the automatic biochemical analyzer (Hitachi-7080): temperature 25°C, reaction time 20 minutes, test main wavelength 340nm, test secondary wavelength 405nm or more, the total amount of the measured carbon dioxide sample and reagent I, reagent II and reagent III The volume ratio is 1:25, the dosage of reagent I, reagent II and reagent...

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Abstract

The invention relates to measuring method for carbon dioxide content and diagnosing kit. The invention directly uses carbon dioxide( bicarbonate radical) and pyruvic acid to produce malic acid by decarboxylation of malic dehydrogenase and oxidizes reduced coenzyme into oxidized type coenzyme, measuring the decent rate of dominant wavelength 340nm absorbance could quantitatively response the carbon dioxide content in sample, thus, the strength of bicarbonate radical could be extrapolated. The method has high specificity, has no pollution from inside and outside material, and accurate test result. The diagnose kit is made up into bi-agent or tri-agent that could reduce cross influence from each component and keeps stability of the agent that is convenient for long-term storage. The method could quickly test on commonness ultraviolet/ visible light, analysis or semi-transfer/ full automatically biochemical study instrument, and doesn't need particularity or extra instrument. The testing cost is cheap, and is convenient for popularization.

Description

technical field [0001] The invention relates to a method for measuring carbon dioxide content by an enzymatic method, and a carbon dioxide diagnostic kit prepared by applying the method, which belongs to the technical field of medical inspection and measurement. Background technique [0002] A constant blood pH is an essential condition for maintaining life activities. There are various buffer systems in blood plasma, the bicarbonate buffer system is the most important, which directly affects the pH value of blood. As is well known, bicarbonate HCO 3′ - with carbon dioxide CO 2 There is a relatively strong corresponding balance relationship between them, therefore, the CO in the blood 2 It plays an important role in regulating the acid-base balance in the human body. blood CO 2 Changes in content mainly reflect metabolic acid-base balance disorders: simple metabolic acid or alkalosis is the result of HCO in the blood 3 - Concentrations decrease or increase, total CO ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/75G01N21/31
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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