Enzymatic method for determining sodium ion content and sodium ion diagnosis kit

A diagnostic kit and sodium ion technology are applied in the field of medical testing and determination, which can solve the problems that a biochemical analyzer cannot measure the content of sodium ions and potassium ions at the same time, cannot be popularized and applied, and are complicated to operate, so as to achieve low testing cost and easy popularization. The effect of simple application and method

Inactive Publication Date: 2006-06-14
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because there are holes in the macrocyclic structure, the oxygen atoms in the molecule have unshared electron pairs that can be combined with metal ions. According to the size of the holes, metal ions of different diameters can be selectively combined to achieve the purpose of measuring the ion concentration; Spectrophotometry can also be used to detect K in serum + 、Na + , but the operation is complicated, the error is large, and it is not as simple as the flame photometry
[0007] The method of enzymatic determination of sodium ion content has a good consistency with the flame photometer method or ion selective electrode method. This method has strong anti-interference and good stability, but the biochemical analyzer cannot simultaneously measure sodium ion and The respective content of potassium ions makes this method unable to be popularized and applied.

Method used

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  • Enzymatic method for determining sodium ion content and sodium ion diagnosis kit
  • Enzymatic method for determining sodium ion content and sodium ion diagnosis kit
  • Enzymatic method for determining sodium ion content and sodium ion diagnosis kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 (single dose)

[0039] Prepare a sodium ion diagnostic kit with the following ingredients and amounts:

[0040] Diglycine buffer 80mmol / l,

[0041] o-Nitrophenol-β-D-galactopyranoside 2mmol / l,

[0042] β-Galactosidase 10000U / l,

[0043] Ethylene glycol 50% (total reagent volume).

[0044] Set on the automatic biochemical analyzer (Hitachi-7080): temperature 37°C, reaction time 10 minutes, test main wavelength 405nm, test sub-wavelength above 505nm, the volume ratio of the tested sodium ion sample to the reagent is 1:25, The reaction direction is a positive reaction (the absorbance increases, the same below).

[0045] Add the sample and the prepared single agent, and the two are automatically mixed inside the analyzer to detect and record the rise of the absorbance at 405 nm. According to the rate method, end point method and other methods, the content of sodium ions in the sample was measured against the corresponding standard curve. ...

Embodiment 2

[0046] Embodiment 2 (one of the two-dose examples)

[0047] Prepare a sodium ion diagnostic kit with the following ingredients and amounts:

[0048] Reagent I——

[0049] Imidazole-hydrochloric acid buffer 100mmol / l,

[0050] o-Nitrophenol-β-D-galactopyranoside 5mmol / l,

[0051] Glycerol 20mmol / l;

[0052] Reagent II——

[0053] Imidazole-hydrochloric acid buffer 100mmol / l,

[0054] β-Galactosidase 20000U / l,

[0055] Ethylene glycol 50% (total volume of Reagent II).

[0056] Set on the automatic biochemical analyzer (Hitachi-7080): the temperature is 30°C, the reaction time is 15 minutes, the test main wavelength is 405nm, and the test sub-wavelength is above 505nm. The total volume of the tested sodium ion sample and reagent I and reagent II is The ratio is 1:25, the ratio of the amount of reagent I to reagent II is 4:1, and the reaction direction is positive reaction.

[0057] Add the sample and reagent I first, and add reagent II after 5 ...

Embodiment 3

[0058] Embodiment 3 (two-agent example)

[0059] Prepare a sodium ion diagnostic kit with the following ingredients and amounts:

[0060] Reagent I——

[0061] Tris-HCl buffer 120mmol / l,

[0062] β-Galactosidase 30000U / l,

[0063] Glycerol 50% (total volume of reagent I);

[0064] Reagent II——

[0065] Tris-HCl buffer 120mmol / l,

[0066] o-Nitrophenol-β-D-galactopyranoside 8mmol / l,

[0067] Propylene glycol 50% (total volume of Reagent II).

[0068] Set on an automatic biochemical analyzer (Hitachi-7080): temperature 37°C, reaction time 10 minutes, test main wavelength 405nm, test sub-wavelength above 505nm, the total volume of the tested sodium ion sample and reagent I and reagent II The ratio is 1:25, the ratio of the amount of reagent I to reagent II is 4:1, and the reaction direction is positive reaction.

[0069] Add the sample and reagent I first, and add reagent II after 5 minutes. The three are automatically mixed inside the analyze...

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Abstract

The invention relates to enzyme method measuring sodium ion content method and its diagnosis kit. It is adopted galactosidase reaction colorimetry to use sodium ion to activate beta-galactosidase enzymolysis o-nitrophenol group-beta-D- pyranoside galactose to make 405nm dominant wavelength absorbance rise. Its absorbance amplitude of fluctuation is proportional to sample sodium ion. The method has high specificity, good accuracy. The diagnosis kit can be made into bi-agent to reduce cross influence. The method can be quickly measured by ultraviolet/visible light analyzer or semi/full automatic biochemical analysis. Its measuring cost is low; and it is convenient for generalization and application.

Description

technical field [0001] The invention relates to an enzymatic method for determining the content of sodium ions in samples such as serum and plasma, and a sodium ion diagnostic kit prepared by using the method, belonging to the technical field of medical inspection and determination. Background technique [0002] The content of sodium ions in serum (abbreviated as "blood sodium") has very important clinical significance. There are three decision levels in medicine, which are: level 1-115mmol / l, level 2-135mmol / l, level 3- - 150mmol / l. When the patient's blood sodium concentration is equal to or lower than level 1, symptoms such as confusion, fatigue, nausea, headache, vomiting and anorexia may appear, indicating that the proportion of water in the body is greater than sodium, and corresponding treatment measures should be taken; when the blood sodium concentration When it is lower than level 2, the cause of hyponatremia should be found, and serum osmolarity, serum potassium ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/34
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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