Method for detecting kiwifruit bacterial canker pathogenic bacteria

Abstract

The invention discloses a method for detecting kiwifruit bacterial canker pathogenic bacteria. The method comprises the following steps: performing amplification and sequencing on all stains of kiwifruit canker pathogenic bacteria by using a bacterial ITS universal primer; performing multiple-homology comparative analysis on ITS sequences, and designing a specific primer and a probe for the kiwifruit canker pathogenic bacteria; and performing usability detection, specificity detection, sensitivity detection taking bacterial liquid as a template, sensitivity detection taking DNA as a template, fluorescence PCR (Polymerase Chain Reaction) detection taking inoculated kiwifruit branches as objects and fluorescence PCR detection taking field pathogenetic samples as objects on the specific primer and the probe for the kiwifruit canker pathogenic bacteria, until a real-time fluorescence PCR primer and a real-time fluorescence PCR probe are obtained. By use of the method disclosed by the invention, pollution and a false positive result during detection can be effectively prevented to obtain the real-time fluorescence primer and probe, establish a real-time quantitative fluorescence PCR detection system for the kiwifruit canker pathogenic bacteria, and achieve rapid, specific, sensitive and quantitative detection of the kiwifruit canker pathogenic bacteria.

Description

technical field [0001] The invention relates to the technical field of kiwifruit planting, in particular to a detection method for kiwifruit bacterial canker. Background technique [0002] Kiwifruit is Actinidiacea (Actinidiacea) genus ( Actinidia ) Deciduous vine fruit tree, its fruit is rich in nutrition, rich in amino acids, proteins, minerals and multivitamins, especially the content of vitamin C ranks first among all kinds of fruits. At the same time, kiwifruit has high medicinal value. The whole plant of kiwifruit can be used as medicine, and the extracts from its roots and fruits have anti-mutation, anti-tumor, and anti-oxidation effects. Kiwi fruit is widely distributed in my country. It is cultivated in Shaanxi, Gansu, and Henan in the north, Guangdong, Guangxi, and Fujian in the south, Guizhou, Yunnan, Sichuan, and Chongqing in the southwest, and all provinces (regions) in the middle and lower reaches of the Yangtze River. [0003] In recent years, due to the r...

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Problems solved by technology

However, these methods are prone to false positive or false negative results, which are likely to cause pollution to the laboratory, and are also likely to cause harm to the body of the experimenter.