32 results about "Bactrocera minax" patented technology

The invention relates to a semi-artificial feed for Bactrocera minax larvae and a preparation method thereof. The semi-artificial feed is composed of the following raw materials: 20-30g of soybean powder, 20-30g of corn powder, 5-10g of sugar, 4-8g of yeast, 0.3-0.4g of vitamin, 0.05-0.1mL of acid, 10-15g of fresh citrus flesh, 0-0.5g of spirulina powder, 8-12mL of fresh citrus juice, 2-5g of toilet paper, 120-140mL of water, and 0.08-0.2g of a preservative. The invention also includes the preparation method of the semi-artificial feed for Bactrocera minax larvae. The semi-artificial feed provided by the invention has the advantages of easily available formula materials, low cost and simple preparation method, and can feed Bactrocera minax larvae in large quantities.

The invention discloses an application of hansenula anomala in preparing citrus fruit fly bait. The invention also discloses citrus fruit fly bait which consists of hansenula anomala hydrolysis fermentation broth and auxiliary bait, wherein the auxiliary bait includes polypeptone, brown sugar, table vinegar or ammonium acetate, preservative and water composition or sweet and sour liquid. The citrus fruit fly bait disclosed by the invention realizes a good effect on inducing bactrocera minax and bactrocera dorsalis as well as a good effect on inducing both the females and males of the bactrocera minax and bactrocera dorsalis, is safe and non-toxic and harmless to human bodies and other beneficial insects, and avoids the residues.

The invention discloses a bactrocera minax adult attractant as well as a preparation method and application of bactrocera minax adult attractant. The bactrocera minax adult attractant comprises the following components: 80-120g of corn flour, 10-20g of dry yeast, 10-20g of brown sugar, 4-6ml of acetic acid, 4-6ml of ethyl acetate, 2-8ml of orange juice or 2-4ml of sweet orange oil or 2-4ml of sweet orange essential oil, 90-120ml of water, 0.6-0.9g of methylparaben or 0.6-0.9g of sodium methylparaben. The invention further comprises the preparation method and application of the bactrocera minax adult attractant. The bactrocera minax adult attractant has obvious attraction effect on the bactrocera minax adult, and can be used for attracting both the male and the female, the effect is especially good for the female. The preparation method of the attractant is easy to implement, the operation is simple and convenient, the raw material is easily available, the preventing effect is stable, the attracting efficiency is high; and the attractant is convenient to use, low in cost and environmentally friendly.

The invention discloses a microbial trapping agent and application thereof. An active component of the trapping agent is bacillus amyloliquefaciens YN2010-KC (CGMCC No. 5722) fermentation liquor in which the living bacteria count is larger than or equal to 3*10<9>cfu / ml. The trapping agent can induce trypetid, vinegar fly and vegetable leafminer to eat due to a particular smell emitted thereby and catch both female and male flies, is long in action period and low in cost, does not contain any chemical toxic agents, does not kill and wound any other non-target living beings and belongs to an environment-friendly biological trapping agent. Euaresta aequalis is trapped: the quantity of the Euaresta aequalis is increased by 18.81 to 23.71% compared with that of the control, and the quantity of female flies trapped by the trapping agent is increased by 37.78% to 30 times compared with that of the control. Tetradacus citri is trapped: the quantity of the Tetradacus citri is increased by 64.43% compared with that of the control, and the quantity of female flies trapped by the trapping agent is increased by 33.25 times. The vegetable leafminer is trapped: the female fly rate accounts for 55 to 60%.

The invention relates to bactrocera minax sex pheromone, and an extraction method and an application thereof. A main active component of the bactrocera minax sex pheromone provided by the invention is undecanol. The extraction method of the bactrocera minax sex pheromone comprises the steps of immersing bactrocera minax sex pheromone in a mating season in ethyl ether for 20 s-300 s; analyzing by using a gas chromatography-mass spectrometer to obtain chemical components of an extract liquid; and determining activities of the chemical components in the extract liquid by using a Y-type olfactometer. The bactrocera minax sex pheromone provided by the invention can be used as a sex attractant for monitoring field occurrence conditions of the bactrocera minax and disturbing mating of the bactrocera minax by mating disruption, and can be used as an attractant to trap and kill the bactrocera minax. The extraction method of the bactrocera minax sex pheromone is simple in operations, high in reproducibility and small in required bactrocera minax amount. Besides, a preparation period is short and result reliability is high.

The invention discloses an artificial feed for adult bactrocera minax, and a preparation method and application thereof. The artificial feed is prepared from the following materials: 24-36g of brown sugar, 8-12g of yeast extract powder or / and yeast paste or / and yeast powder and 200-400g of distilled water. The invention also discloses the preparation method and the application of the artificial feed. The artificial feed disclosed by the invention has the advantages that the materials are easily available, the operation is simple, the cost is low, the life of adult bactrocera minax is long, the fertility of adult bactrocera minax is high and the environmental-friendly effect is achieved.

The invention discloses a pesticide capable of effectively repelling bactrocera minax female adult pests and an application of the pesticide. According to the invention, experiments discover that combined usage of methyleugenol and 4-(4-acetoxyphenyl)-2-butanone has a remarkable repellent effect on the bactrocera minax female adult pests, so that combination of the methyleugenol and the 4-(4-acetoxyphenyl)-2-butanone can be utilized as a repellent agent of the bactrocera minax female adult pests, and a novel idea is supplied for comprehensive prevention and control of bactrocera minax.

The invention relates to the field of molecular biology, and in particular relates to an endogenous reference gene BM-EF1-alpha segment of bactrocera minax and an application thereof. The nucleotide sequence of the gene segment is shown by SEQ ID No.1. According to the BM-EF1-alpha of bactrocera minax, provided by the invention, the nucleotide sequence with 224bp can be used as a functional gene for studying bactrocera minax in future and an endogenous reference gene for gene expression in different development stages.

The invention relates to a method for treating bactrocera minax fruits. The method mainly comprises the following steps: preparing wormy fruit treatment bags; bagging the wormy fruits; sealing; exposing the wormy fruits in the sun; and sealing. By the steps, the bactrocera minax fruits can be prepared into return fertilizer applied to a citrus garden, and the modes of burying deeply, boiling in water, burning and performing medicine treatment in the conventional treatment on the bactrocera minax fruits are not required, so labor force is saved, the amount of labor used for treatment on each mu of bactrocera minax fruits is reduced from more than 2 in the past to 0.5; the operation procedures are simple and convenient and the treatment process has 2 to 3 procedures less than that of other methods, so operators are easy to accept; the cost is reduced and the cost of transportation, pesticide, construction of a treatment pond and the like are saved compared with that of other methods; and waste is changed into treasures, the residues of the wormy fruits are utilized reasonably, and environmental pollution is reduced.

The invention discloses a remote monitoring device and method for the occurrence dynamic state of an adult bactrocera minax population. The remote monitoring device for the occurrence dynamic state of the adult bactrocera minax population comprises a square insect breeding cage made of gauze, wherein a square insect sticking box is arranged at the upper part in the insect breeding cage; an attractant box and fly trapping balls are arranged in the insect sticking box; the four surfaces of the insect sticking box are provided with adhesion layers and smell diffusion holes; a control box is arranged on a supporting rod; a camera for shooting the outer surface of the insect sticking box is arranged on the horizontal rod; a wireless transmission module and a controller are arranged in the control box; and the wireless transmission module and the camera are both electrically connected with a controller. The population monitoring method comprises the steps of collecting damaged fruits, installing equipment, shooting trapping photos regularly every day, identifying and counting the number of insect pests through the trapping photos, and monitoring and analyzing the occurrence dynamic state of the insect pest population according to the change of the number. The remote monitoring device and method for the occurrence dynamic state of the adult bactrocera minax population provided by the invention have the advantages that the method is simple, convenient, small in interference of miscellaneous insect pests, high in intelligent degree and the like, and a scientific and effective method and a technical support are provided for intelligent monitoring and early warning of bactrocera minax pests in modern agricultural citrus production.

The invention relates to the field of molecular biology, particularly to the application of a G6PDH gene segment taken as a stable expression reference gene of a Bactrocera minax under the intimidation condition. According to the invention, the selected G6PDH ( glucose-6-phosphate dehydrogenase) reference gene is subjected to semiquantitative RT-PCR technology detection, as a result, the fact that both an imago under high temperature of 35 DEG C and irradiation condition and a larva under low temperature of 4 DEG C intimidation condition can express stably is shown, so that the application lays a foundation for the research of Bactrocera minax function and the expression of Bactrocera minax under different intimidation conditions.

The invention relates to an artificial feeding method for holonomic Bactrocera minax, larva artificial feed and a preparation method of the larva artificial feed. The artificial feeding method includes the steps of firstly, in the larva stage, placing Bactrocera minax larvae into a culture dish loaded with Bactrocera minax larva artificial feed to perform artificial feeding; secondly, in the pupalstage, placing the matured Bactrocera minax larvae into feeding soil to perform pupation; thirdly, in the adult stage, placing the pupated Bactrocera minax adults into an adult culture chamber, and feeding the adults with sugar water until spawning to create the next generation. By the artificial feeding method, the breeding cycle of the holonomic Bactrocera minax can be shortened, the survival rate of the artificially fed larvae is evidently increased, and constant artificial feeding of the eggs, larvae, pupae and adults of the Bactrocera minax can be achieved. The larva artificial feed is easy in raw material obtaining, low in cost and simple in preparation method.

The invention discloses a medicine formula for preventing and treating Bactrocera minax, and the medicine comprises the following components in parts by weight: 40-60 parts of rheum officinale, 20-40 parts of polygonum multiflorum, 10-20 parts of radix achyranthis bidentatae, 5-15 parts of Phytolacca acinosa, 10-20 parts of Ageratina adenophora, 5-15 parts of Tripterygium wilfordii and 3-12 parts of common andrographis herb; and the botanical insecticide disclosed by the invention has significant effects on aphid, red spiders, borers and fruit flies on fruit trees.

The invention provides a method for testing young-citrus-fruit-originated volatile matters having effects on adult Bactrocera minax. According to the results of the testing method, the young-citrus-fruit-originated volatile matters including limonene, 4-terpene alcohol, nonanal and citral have behavioral reaction effects on adult Bactrocera minax; limonene, 4-terpene alcohol and citral all have effects on male citrus Bactrocera minax; and limonene and nonanal both have effects on female citrus Bactrocera minax. It is confirmed for the first time that the young-citrus-fruit-originated volatilematters having effects on male and female adults of Bactrocera minax include limonene, 4-terpene alcohol, nonanal and citral, and the young-citrus-fruit-originated volatile matters can be further applied to research and development of attractants and repellents and play a positive role in pollution-free prevention and treatment in citrus production areas.

The invention relates to a preparation method of an adult bactrocera minax attractant, and belongs to the technical field of bactrocera minax control drugs. The adult bactrocera minax attractant is researched and developed by utilizing the characteristic that bactrocera minax adults like Chinese chestnut male inflorescences, so that compared with an existing attractant prepared by utilizing the common habit that phytophagous insects have tropism on sugar, wine and vinegar tastes and the adults need to be supplemented with nutrients after eclosion, the attractant has a better attraction effect;therefore, the purposes of enhancing the strong trapping and killing efficiency of bactrocera minax adults and improving the prediction accuracy of bactrocera minax are achieved, and the use requirements of people are met.

The invention discloses a nuisanceless treating pond for bactrocera minax maggot orange fruits. Guiding holes are disposed in the bottom of a hardening pond, and filter screens are installed in the guiding holes. The hardening pond is formed in a concrete pouring mode. The hardening pond is used for containing maggot fruits or waste fruits. According to the nuisanceless treating pond, an environment which is hostile for the survival of bactrocera minax is created according to the law of demand for environment conditions of the bactrocera minax, and the economical, efficient and safe control purpose is achieved.

The invention discloses an LAMP (loop-mediated isothermal amplification) primer group for identifying bactrocera minax and application of the LAMP primer group. The LAMP primer group is composed of a prime-F3, a prime-B3, a primer-FIP and a primer-BIP, and nucleotide sequences of the LAMP primer group are sequentially shown as a sequence 1 to a sequence 4. The LAMP primer group provided by the invention can be used for identifying whether to-be-detected fruit flies are or are candidates of citrus fruit flies, and can also be used for detecting whether to-be-detected samples contain or are candidates of citrus fruit flies. The LAMP primer group is adopted for identifying the bactrocera minax, high specificity and high sensitivity are achieved, and simple, convenient, rapid and accurate detection can be achieved. The method has great application value.

The invention discloses a pest control method for Chinese prickly ash, and relates to the technical field of Chinese prickly ash planting. Comprising the following steps: S1, soil preparation and fertilization; s2, early-stage disease and pest control; s3, selection and treatment of seedlings; s4, planting pepper seedlings; s5, topdressing the pepper seedlings; s6, middle-stage disease and pest control; s7, pruning branches and leaves for the first time; s8, disease and pest control in the middle and later periods; s9, later-stage disease and pest control; s10, pruning branches and leaves for the second time; and S11, harvesting fruits. Diseases harmful to pepper mainly include anthracnose, pests mainly include aphids, bactrocera minax, scale insects and pepper tiger beetles, branches and leaves are reasonably pruned, good ventilation and light transmission of tree crowns are kept, measures such as pruning of branches with diseases and pests and removal of dry branches and fallen leaves are adopted to inhibit or reduce the occurrence of diseases and pests, and in different growth periods of pepper, the pepper is planted in a greenhouse. Chemical liquid medicine and biological liquid medicine for disease and pest control are sprayed for pesticide control, so that various germs are killed, and a remarkable killing effect on common diseases and pests in pepper planting is achieved.

The invention discloses a method for identifying bactrocera minax and a primer group used by the method. The primer group is composed of a prime-F3, a prime-B3, a primer-FIP and a primer-BIP, and nucleotide sequences of the primer group are sequentially shown as a sequence 1 to a sequence 4. The loop-mediated isothermal amplification technology for identifying the bactrocera minax can realize rapid identification of eggs, larvae / adult individuals of the bactrocera minax, has the advantages of rapidness, high efficiency, strong specificity, high sensitivity, low cost, simplicity and convenience in operation and no need of special instruments, is suitable for the field, is easy to popularize in the basic level, and has wide application prospects. A reliable technical basis can be provided for prevention and control of the citrus fruit flies, and the method has important significance on plant quarantine and citrus fruit trade.

The invention relates to the technical field of agricultural pest control, in particular to a method for biologically controlling Bactrocera minax (Enderlein). According to the method, bacteria bait is prepared from ascosphaerales to induce Bactrocera minax (Enderlein) to eat; ascosphaerales can survive in the intestinal canals of Bactrocera minax (Enderlein), germinate and expand into spheres in the intestinal canals, and stretch out of germ tubes to form hyphae and generate spores; the hyphae perforate the hypodermal cells of the inner walls of the intestinal canals; lesions occur in fat body tracheae and muscles; and the hyphae multiply enormously in body cavities to kill Bactrocera minax (Enderlein). The method provided by the invention can be used for solving the problem that conventional pesticides can hardly kill Bactrocera minax (Enderlein) and can cause pesticide residue pollution, reduces environmental pollution caused by mass and repeated application of chemical pesticides, and protects production, users and other beneficial organisms; and the raw material is easy to obtain, the preparation is simple, the control effect is high, and the popularization is easy.

The trapping and killing fake fruit comprises a fake fruit body, the fake fruit body is a hollow sphere formed by a plastic material, the outer surface of the fake fruit body is a suede surface, and the fake fruit body is smaller than the average fruit size of the citrus fruit tree and has the same color as a mature fruit of the citrus fruit tree; a lure with a sex attractant is arranged in an inner hollow cavity of the fake fruit body, and micropores are formed in the spherical surface of the fake fruit body; a concave part is further arranged on the surface of the fake fruit body, the concave part is filled with a filling material which is prepared by bonding a protein attractant, a food attractant and a pomelo extract through a sizing material, the surface of the filling material and the surface of the fake fruit body form a continuous outer surface, and the continuous outer surface is coated with an adhesive layer. The pesticide can be used for effectively preventing and treating bactrocera minax and bactrocera minax, and is environment-friendly, high in trapping and killing efficiency and stable and obvious in effect.

The invention discloses a drone prevention and control method for bactrocera minax. When a drone sprays pesticide liquid, a spaced strip-shaped spraying mode is adopted, during spraying, the drone islocated above one shady side of a bactrocera minax tree, and the part accounting for 1 / 4-1 / 2 of the area of the crown of the bactrocera minax tree is sprayed. The distance between a nozzle of the drone and the top of the bactrocera minax tree is 50-100 cm. Every 1,000 parts of the pesticide liquid contain 1 part of 90% crystal dipterex and 0.5-1.5 parts of brown sugar. By means of the method, thework efficiency can be effectively improved by 30-40 times, the labor cost is reduced by 10-30%, and pesticide is saved by 20-30%. Besides, the method can also effectively solve a series of problems that there is a shortage of labor and efficiency and a low work efficiency ratio for completing large-area prevention and control within a set time.