Microbial trapping agent and application thereof

A trapping agent and microbial technology, applied in the application of microbial trapping agent, Liriomyza sativa, and the field of trapping male and female fruit flies, can solve the problem of strong drug resistance, difficult control, and the lack of real application and benefit of biological control technology in production, etc. problems, to achieve the effect of long action period, strong competitiveness and significant ecological benefits

Active Publication Date: 2016-03-23
MICROBIAL FERMENTATION ENG RES CENT CO LTD OF YUNNAN PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Due to the serious overlapping of generations of this insect and its strong drug resistance, it is difficult to control it in production.
At present, the control of Liriomyza sativae in production is still mainly based on chemical control. In order to solve the food safety problems caused by chemical pesticides for many years, biological control measures should be used to control insect pests, but biological control technology has not really been used. It is imminent to find a new lure that is highly targeted, highly effective, safe for humans and animals, and has no environmental pollution in terms of production application and benefit.

Method used

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  • Microbial trapping agent and application thereof
  • Microbial trapping agent and application thereof
  • Microbial trapping agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Acquisition, Identification and Preservation of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YN2010-KC

[0028] 1. Obtaining of Bacillus amyloliquefaciens YN2010-KC of the present invention

[0029] 1.1 Materials

[0030] The soil was collected from the soil around the corn rhizosphere of the Houshan Farm of Yunnan Agricultural University.

[0031] 1.2 Culture medium preparation

[0032] Melt LB solid medium and PSA medium (potato sucrose agar medium), pour them into sterilized petri dishes, cool down, and make LB and PSA plates.

[0033] 1.3 Isolation and culture

[0034] A strain isolated and purified was named YN2010-KC by using dilution plate separation method and plate streak separation.

[0035] 2. Identification of Bacillus amyloliquefaciens YN2010-KC

[0036] 2.1. Morphological identification

[0037] The bacterial strain YN2010-KC was cultured on LB medium, cultured at 37°C for 36 hours, and then observed and identified by morphologic...

Embodiment 2

[0042] Embodiment 2 The preparation method of microorganism trapping agent of the present invention, comprises the following steps:

[0043] (1) Inoculate the Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YN2010-KCCGMCCNo.5722 stored at -80°C on liquid LB medium, and culture it at 35°C for 24 hours to obtain activated Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YN2010-KCCGMCCNo.5722, Inoculate the activated Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YN2010-KCCGMCCNo.5722 into the seed culture medium at a 10% inoculum amount, and cultivate it on a shaking table for 36 hours at a culture temperature of 35°C and a rotation speed of 120 rpm / min to obtain a first-grade Seed liquid, the seed medium is liquid LB medium, pH7.0~7.2;

[0044] (2) Transfer the primary seed solution to the secondary seed medium in the seed tank at 35°C with a rotation speed of 100 rpm / min, an air flow of 1000 L / h, and a tank pressure of 0.02 to 0.03 at 10% inoculum size....

Embodiment 3

[0047] Embodiment 3 The comparative test of microbial trapping agent of the present invention and other attractants trapping Bactrocera citrus

[0048] Carry out trapping effect test in the citrus orchard of Yuanjiang County, Yunnan Province, simultaneously with " Guo Ruite " fruit fly attractant, (belong to albumen bait, commercially available), methyl eugenol (property attractant, commercially available) and water ( blank control) for comparison. The dosage of the microorganism trapping agent of the present invention is 50ml / bottle, the dosage of the fruit fly attractant is 50ml / bottle, and the dosage of methyl eugenol is 3ml / bottle.

[0049] Each plastic bottle is filled with the above-mentioned attractant, the bottle cap is tightened, and 4 holes are evenly distributed on the bottle wall 2 cm away from the liquid surface in the bottle, and the aperture of each hole is 5 mm. Field release was carried out at noon on May 16, 2015, and the plastic bottles were hung 0.5-1.5 me...

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Abstract

The invention discloses a microbial trapping agent and application thereof. An active component of the trapping agent is bacillus amyloliquefaciens YN2010-KC (CGMCC No. 5722) fermentation liquor in which the living bacteria count is larger than or equal to 3*10<9>cfu/ml. The trapping agent can induce trypetid, vinegar fly and vegetable leafminer to eat due to a particular smell emitted thereby and catch both female and male flies, is long in action period and low in cost, does not contain any chemical toxic agents, does not kill and wound any other non-target living beings and belongs to an environment-friendly biological trapping agent. Euaresta aequalis is trapped: the quantity of the Euaresta aequalis is increased by 18.81 to 23.71% compared with that of the control, and the quantity of female flies trapped by the trapping agent is increased by 37.78% to 30 times compared with that of the control. Tetradacus citri is trapped: the quantity of the Tetradacus citri is increased by 64.43% compared with that of the control, and the quantity of female flies trapped by the trapping agent is increased by 33.25 times. The vegetable leafminer is trapped: the female fly rate accounts for 55 to 60%.

Description

technical field [0001] The invention belongs to the technical field of pest control in agricultural science, and in particular relates to a microbial trapping agent and its application in trapping male and female fruit flies and Liriomyza sativae. Background technique [0002] Diptera is an order of Arthropoda, Mandibulata, Insecta, and Pterygota. It is second only to Coleoptera and Lepidoptera in Insecta. The fourth largest order of the order Hymenoptera. There are 85,000 known species in the world, with global distribution. More than 4000 species are known in China. Insects of the order Diptera have a pair of wings and a pair of balance bars, including mosquitoes, flies, and gadflies. The larvae of some groups of common Diptera, such as seed fly, leaf miner, fruit fly, wheat gall midge, etc., are important agricultural pests. Liriomypidae damage a variety of leguminous plants; many species of Tephritidae damage citrus, pears, peaches, etc. [0003] Tephritidae pests b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P19/00C12N1/20C12R1/07A01N63/22
CPCC12N1/20A01N63/22C12R2001/07C12N1/205
Inventor 何月秋吴毅歆何鹏搏谢兰芬杨发祥
Owner MICROBIAL FERMENTATION ENG RES CENT CO LTD OF YUNNAN PROVINCE
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