Microbial trapping agent and application thereof
A trapping agent and microbial technology, applied in the application of microbial trapping agent, Liriomyza sativa, and the field of trapping male and female fruit flies, can solve the problem of strong drug resistance, difficult control, and the lack of real application and benefit of biological control technology in production, etc. problems, to achieve the effect of long action period, strong competitiveness and significant ecological benefits
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Embodiment 1
[0027] Example 1 Acquisition, Identification and Preservation of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YN2010-KC
[0028] 1. Obtaining of Bacillus amyloliquefaciens YN2010-KC of the present invention
[0029] 1.1 Materials
[0030] The soil was collected from the soil around the corn rhizosphere of the Houshan Farm of Yunnan Agricultural University.
[0031] 1.2 Culture medium preparation
[0032] Melt LB solid medium and PSA medium (potato sucrose agar medium), pour them into sterilized petri dishes, cool down, and make LB and PSA plates.
[0033] 1.3 Isolation and culture
[0034] A strain isolated and purified was named YN2010-KC by using dilution plate separation method and plate streak separation.
[0035] 2. Identification of Bacillus amyloliquefaciens YN2010-KC
[0036] 2.1. Morphological identification
[0037] The bacterial strain YN2010-KC was cultured on LB medium, cultured at 37°C for 36 hours, and then observed and identified by morphologic...
Embodiment 2
[0042] Embodiment 2 The preparation method of microorganism trapping agent of the present invention, comprises the following steps:
[0043] (1) Inoculate the Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YN2010-KCCGMCCNo.5722 stored at -80°C on liquid LB medium, and culture it at 35°C for 24 hours to obtain activated Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YN2010-KCCGMCCNo.5722, Inoculate the activated Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YN2010-KCCGMCCNo.5722 into the seed culture medium at a 10% inoculum amount, and cultivate it on a shaking table for 36 hours at a culture temperature of 35°C and a rotation speed of 120 rpm / min to obtain a first-grade Seed liquid, the seed medium is liquid LB medium, pH7.0~7.2;
[0044] (2) Transfer the primary seed solution to the secondary seed medium in the seed tank at 35°C with a rotation speed of 100 rpm / min, an air flow of 1000 L / h, and a tank pressure of 0.02 to 0.03 at 10% inoculum size....
Embodiment 3
[0047] Embodiment 3 The comparative test of microbial trapping agent of the present invention and other attractants trapping Bactrocera citrus
[0048] Carry out trapping effect test in the citrus orchard of Yuanjiang County, Yunnan Province, simultaneously with " Guo Ruite " fruit fly attractant, (belong to albumen bait, commercially available), methyl eugenol (property attractant, commercially available) and water ( blank control) for comparison. The dosage of the microorganism trapping agent of the present invention is 50ml / bottle, the dosage of the fruit fly attractant is 50ml / bottle, and the dosage of methyl eugenol is 3ml / bottle.
[0049] Each plastic bottle is filled with the above-mentioned attractant, the bottle cap is tightened, and 4 holes are evenly distributed on the bottle wall 2 cm away from the liquid surface in the bottle, and the aperture of each hole is 5 mm. Field release was carried out at noon on May 16, 2015, and the plastic bottles were hung 0.5-1.5 me...
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