The invention provides a culture medium for insect cell growth, which is characterized in that the culture medium per 1,000ml comprises the following components: 40-50g of Grace culture medium, 0.5-3g of trehalose, 0.5-3g of yeast extract, 100-200ml of fetal calf serum and the balance of water. The culture medium obtained by using the technical scheme has the advantages of scientific and reasonable formulation, easily obtained raw materials and simplicity in a preparation method; after 14 insect cells are cultured, a result shows that may insect cells from lepidoptera, coleoptera, orthoptera, hymenoptera, diptera, homoptera and the like are suitably cultured by using the culture medium provided by the invention, the majority of cells can grow in the culture medium provided by the invention, and a plurality of finite cell lines and infinite cell lines are successfully established.