Beauveria bassiana strain with broad-spectrum insecticidal property and application thereof

A technology of Beauveria bassiana and strains, applied in the application, insecticide, biocide and other directions, can solve the problem of lack of broad-spectrum Beauveria bassiana strains, and achieve non-toxicity, strong pathogenicity, The effect of high pathogenicity

Active Publication Date: 2021-11-23
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] A review of technical research and application progress shows that at present, there is a lack of broad-spectrum Beauveria bassiana strains, and there is no simultaneous treatment of Coleoptera, Hemiptera, and Hymenoptera pests, such as flea beetle, cabbage aphid, and tobacco powder. Lice, fire ants and citrus psyllids all have highly active insecticidal fungal strains

Method used

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  • Beauveria bassiana strain with broad-spectrum insecticidal property and application thereof
  • Beauveria bassiana strain with broad-spectrum insecticidal property and application thereof
  • Beauveria bassiana strain with broad-spectrum insecticidal property and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1: Isolation, purification and identification of Beauveria bassiana BbPs01 strain

[0044] (1) Isolation and purification of bacterial strains: The flea beetles were collected from the cabbage field of Qilin Farm, South China Agricultural University, Guangzhou City, Guangdong Province, and the bacterial layer on the beetles was picked with a sterilized inoculation needle and inoculated on a PDA plate. Then, put it into a 25°C incubator for cultivation, and after the colonies grow, single spores are isolated and cultured according to conventional methods to obtain a pure line, which is transferred to a PDA slant for 7 days and stored in a 4°C refrigerator. The above operations were performed in a sterile environment on an ultra-clean workbench.

[0045] (2) Morphological identification of strains: The isolated and purified Beauveria bassiana strains were cultured on PDA medium with a diameter of 90 mm at 25°C, and white hyphae slices were picked for 4-5 days to o...

Embodiment 2

[0050] Example 2: Activity comparison between Beauveria bassiana BbPs01 strain and other strains

[0051] (1) strain culture

[0052] Get the slant strain of the test strain, scrape a small amount of conidia and inoculate it on the PDA plate, cultivate about 14d in a 25°C incubator, and scrape the colony surface conidia for subsequent use.

[0053] (2) Molecular spore suspension preparation

[0054] Place the conidia scraped in step (1) into a centrifuge tube, add 0.02% Tween-80 solution, shake well, measure the spore concentration with a hemocytometer, and prepare 1 × 10 7 Spore suspension of spores / mL, set aside.

[0055] (3) Determination of biological activity

[0056] Under laboratory conditions, the test insects were treated with the spore suspension of the test strain by dipping method, and treated with 0.02% Tween-80 solution as a control, 20 insects for each treatment, and three biological replicates. After treatment, put them in a rearing box, feed them with fres...

Embodiment 3

[0063] Example 3: Toxicity determination of Beauveria bassiana BbPs01 strain to adult flea beetles

[0064] Using the conidia produced in the above Example 2, under the conditions in the laboratory, different concentrations of spore dipping method were used to treat F. 20 animals were treated with 3 biological replicates. After treatment, put them into rearing boxes, add fresh radish pieces to each rearing box, and replace them every three days; 25 ° C, L:D = 14:12, RH>85% under the conditions of rearing, and investigate the incidence of adult flea beetles every day. Mortality, using SPSS software to analyze and calculate corrected mortality, lethal concentration (LC) 50 ) and lethal time (LT 50 ).

[0065] At the same time of bioassay, the process of Beauveria bassiana BbPs01 strain infecting adult flea beetles was observed, and the morphological metamorphosis of flea beetles was observed under a stereo microscope.

[0066] Cumulative mortality (%) = cumulative number of ...

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Abstract

The invention relates to a Beauveria bassiana BbPs01 strain with a broad-spectrum insecticidal property and an application of the Beauveria bassiana BbPs01 strain, and belongs to the technical field of biological pesticides. The strain BbPs01 has been deposited in Guangdong Microbial Culture Collection Center (GDMCC) at February 4, 2021 with a deposit number of GDMCC NO: 61494. The strain BbPs01 has high activity on pests such as phyllotreta striolata of coleoptera, rhopalosiphum padi, diaphorina citri and bemisia tabaci of hemiptera, solenopsis invicta of hymenoptera and the like; has a potential of serving as a fungal pesticide, has characteristics of no pollution to the environment and no toxicity to human and livestock as a biological living pesticide, and is suitable for the production requirements of organic agricultural products.

Description

technical field [0001] The invention relates to a broad-spectrum insecticidal Beauveria bassiana strain and application thereof, belonging to the technical field of biological pesticides. Background technique [0002] Beauveria bassiana is a widely used entomopathogenic fungus that plays an important role in pest biological control. Beauveria bassiana insecticides have been registered and applied in many countries and regions outside the United States, Europe, Brazil, and India. At present, 25 species of Beauveria bassiana insecticides have been registered in my country, which are used to control locusts, grubs, leek maggots, thrips, tea leafhoppers, pine caterpillars, diamondback moths, corn borers, rice leaf rollers, and diphtheria Borer and other pests, but the application scope of each product is limited (http: / / www.chinapesticide.org.cn / ). There are thousands of species of host insects of Beauveria bassiana, but their pathogenicity is differentiated. The host range of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/30A01P7/04C12R1/645
CPCA01N63/30Y02A50/30
Inventor 胡琼波蒲新华王菁菁
Owner SOUTH CHINA AGRI UNIV
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