Method for directly obtaining natural biotoxin from frozen insect bodies by quickly freezing honeycomb

A technology of toxins and refrigerants, applied in the field of biomedicine, can solve the problems of unfavorable preservation of the original structure of bee venom macromolecules, easy deterioration of bee venom biochemical substances, and easy contamination of venom on the wall of poison sacs, so as to achieve convenient source of raw materials Easy to obtain, easy to freeze, and significant social and economic benefits

Inactive Publication Date: 2014-03-26
DALI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The defect of this method is: when the bee body is dissected at normal temperature, the venom is easily contaminated on the wall of the poison sac;
Furthermore, when unfrozen bee bodies are operated for a long time, the bee venom biochemical substances in the dead bee body venom sacs are prone to deterioration, protein denaturation, polypeptide oxidation and other unfavorable conditions, which are not conducive to the preservation of the original structure of bee venom macromolecules

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Vespa mandarinia Smith's experiment of "freezing and stripping the poison sac for comprehensive poison collection" experiment 1.1 Vespa venom collection location: in the forest of Lianghe County, Dehong Prefecture, Yunnan Province, at an altitude of 1382 meters. The bee body specimen was identified as Vespa mandarinia Smith (Golden Ring Vespa, also known as Golden Hornet) according to Professor Mao Benyong of the National and Local Joint Engineering Research Center for the Development of Medicinal Special Insects, Dali University, Yunnan Province.

[0021] 1.2 Equipment for collecting wasp venom: refrigerant (liquid nitrogen) storage tank, low-temperature resistant pressurized pump and nozzle, low-temperature operating room (cold room with a room temperature of 4 degrees Celsius), surgical scissors, scalpel, surgical forceps, brown glass bottle, large Tweezers, Tris-Buffer buffer.

[0022] 1.3 Method of collecting wasp venom: While wearing protective clothin...

Embodiment 2

[0024] Example 2: Routine toxin collection test of Vespa mandarinia Smith, Vespa mandarinia Smith

[0025] 2.1 Place of collection of wasp venom: a nest of golden hornets with a similar size and growth state environment at a distance of 2.1 kilometers from the honeycomb under the item of embodiment 1.1. The bee body specimen was identified as Vespa mandarinia Smith (Golden Ring Vespa, also known as Golden Hornet) according to Professor Mao Benyong of the National and Local Joint Engineering Research Center for the Development of Medicinal Special Insects, Dali University, Yunnan Province.

[0026] 2.2 Device for collecting wasp venom: use the same batch of auxiliary cover-type bee venom collector modified by the engineering research center, with a wooden frame, 80 cm long and 50 cm wide; embedded with 4 pieces of 20 cm long / 33 cm wide poisonous glass ; Input voltage 12V, output automatic intermittent pulse voltage.

[0027] 2.3 Method of collecting wasp venom: place the poi...

Embodiment 3

[0029] Example 3: A comparative experiment on the amount of bee venom obtained from the conventional venom collection of Polistes sagittarius Saussure and the technique of "freezing and peeling off venom sacs for comprehensive venom collection"

[0030] 3.1 Experimental location: Funing County, Wenshan Prefecture, Yunnan. The two broods of yellow skirt hornets are about 1.9 kilometers apart. In the mountains and forests. The bee body specimen was identified as Polistes sagittarius Saussure by Professor Yang Zizhong of the National and Local Joint Engineering Research Center for the Development of Medicinal Special Insects, Dali University, Yunnan Province.

[0031] 3.2 Conventional venom collection device and method: the wasp venom collection device is the same as that described in 2.2 of Example 2, using the same batch of secondary cover type bee venom collector modified by this engineering research center, with a wooden frame, 80 cm long and 50 cm wide ; Embedded with 4 ...

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PUM

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Abstract

The invention relates to a method for directly obtaining natural biotoxin from frozen insect bodiesby quickly freezing a honeycomb. The method is characterized in that a net and hymenoptera apocrita aculeata insects and the insect bodies are directly frozen through a freezing agent at the temperature lower than minus 20 DEG C, and toxin-class active substances extracted in a frozen state keep the natural structures and can be used for preparing medicaments, daily articles and medical instruments which are used for resisting viruses, bacteria, rheumatism, cancers and cardiovascular and cerebrovascular diseases. The method disclosed by the method is suitable for a key safety toxin collection technology for hymenoptera insects such as long-bellied vespidae, ling-bellied vespidae, polybiidae, narrow-bellied vespidae, polistidae, vespidae and formicidae in the apoidea and the vespoidea. According to the key technology, the problem of bottleneck in collection of the bee venom of bees and the vespoidea can be solved; the key technology has an obvious economical value and can meet the market shortage and overcome the shortcomings in the innovative toxin collection technology.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular, the invention relates to a kind of refrigerant equal to or lower than minus 20 ℃ for honeycombs and insect bodies of bee insects such as bees and wasps. The biomedical poisoning method is carried out directly and rapidly freezing, and obtaining toxin-like active substances from worm bodies in a frozen state. It is generally suitable for needle-tailed bee insects of the Hymenoptera slender suborder, including but not limited to the long-bellied Vespidae, the bell-bellied Vespidae, the Heterogastric Vespidae, the Stenogastric Vespidae, The key technology of safe poison collection of Vespaidae and Vespaidae. Background technique [0002] Hymenoptera (Hymenoptera), Apocrita (Apocrita), Aculeata insects have three superfamilies (Vespoidea), Sphecoidea (Sphecoidea) and Apoidea (Apoidea). Tens of thousands of bee insects, most of which have poison sacs and pincers, can secrete bee v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/64A01K59/00
Inventor 赵昱赵婉宸江滇郭云胶刘建平
Owner DALI UNIV
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